紫外辐射对东北红豆杉鲜叶中紫杉醇及三尖杉宁碱含量的影响

doi:10.7525/j.issn.1673-5102.2007.04.024

›› 2007, Vol. 27 ›› Issue (4): 500-503.doi: 10.7525/j.issn.1673-5102.2007.04.024

紫外辐射对东北红豆杉鲜叶中紫杉醇及三尖杉宁碱含量的影响

李双明;孙　蕊;骆　浩;孙　愫;王　莹;付玉杰^*

东北林业大学森林植物生态学教育部重点实验室，哈尔滨　150040

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-07-20 发布日期:2007-07-20
通讯作者: 付玉杰

The Influence of UV Radiation on the Contents of Paclitaxel and Cephalomannine in the Fresh Leaves of Taxus cuspidata

LI Shuang-Ming;SUN Rui;LUO Hao;SUN Su;WANG Ying;FU Yu-Jie*

Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin　150040

Received:1900-01-01 Revised:1900-01-01 Online:2007-07-20 Published:2007-07-20
Contact: FU Yu-Jie

摘要/Abstract

摘要： 分别采用254、365 nm两种波长的紫外光对东北红豆杉鲜叶进行辐射，研究了波长、辐射时间以及样品处理方式对东北红豆杉鲜叶中紫杉醇及三尖杉宁碱含量变化的影响。结果表明，东北红豆杉鲜叶经匀浆处理后接受紫外辐射，两种波长的紫外光都可以使紫杉醇及三尖杉宁碱的含量增加，但不同波长对紫杉醇及三尖杉宁碱含量提高的趋势却不相同。365 nm的紫外光辐射2 h时使紫杉醇和三尖杉宁碱含量均提高到了最大值，两种物质含量分别提高了44.6%和53.0%，而254 nm的紫外光在辐射8 h时才达到最大值，两种物质含量分别提高了39.2%和24.3%。可以选取365 nm的紫外光对东北红豆杉鲜叶水匀浆体系进行辐射，快速高效地提高鲜叶内紫杉醇和三尖杉宁碱的含量。

关键词: 东北红豆杉, 紫杉醇, 三尖杉宁碱

Abstract: The influence of UV wavelength, time of radiation and the processing method of sample on the content of paclitaxel and cephalomannine in the fresh leaves of Taxus cuspidata was studied in the article, in which two wavelength of 254 nm and 365 nm were used. The results showed that two kinds of UV radiation could enhance the content of two compounds in the fresh leaves of Taxus cuspidata by radiating the water homogenate of the fresh leaves. But, the increase trend of two compounds was different under diverse UV radiation. The wavelength of 365 nm made the content of two compounds achieve the maximum in 2 hours. And the rate of increase were 44.6% for paclitaxel and 53.0% for cephalomannine. While the wavelength of 254 nm needed 8 hours. The rate of increase were 39.2% for paclitaxel and 24.3% for cephalomannine. Thus，the content of paclitaxel and cephalomannine in the fresh leaves of Taxus cuspidata can be enhanced rapidly and effectively by radiating the water homogenate of the fresh leaves under UV radiation.

Key words: Taxus cuspidata, paclitaxel, cephalomannine, UV radiation

李双明;孙　蕊;骆　浩;孙　愫;王　莹;付玉杰*. 紫外辐射对东北红豆杉鲜叶中紫杉醇及三尖杉宁碱含量的影响[J]. , 2007, 27(4): 500-503.

LI Shuang-Ming;SUN Rui;LUO Hao;SUN Su;WANG Ying;FU Yu-Jie*. The Influence of UV Radiation on the Contents of Paclitaxel and Cephalomannine in the Fresh Leaves of Taxus cuspidata[J]. , 2007, 27(4): 500-503.

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紫外辐射对东北红豆杉鲜叶中紫杉醇及三尖杉宁碱含量的影响

The Influence of UV Radiation on the Contents of Paclitaxel and Cephalomannine in the Fresh Leaves of Taxus cuspidata

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