薰衣草腺毛非特异性脂质转移蛋白nsLTP2介导挥发性有机物的细胞壁转运

doi:10.7525/j.issn.1673-5102.2026.02.003

摘要/Abstract

摘要：

薰衣草（Lavandula angustifolia）的香味主要来源于其体表密布的腺毛分泌的挥发性有机化合物（volatile organic compounds，VOCs）。疏水性VOCs从合成部位需穿越亲水性细胞壁，转运至腺毛分泌腔储存。然而，薰衣草疏水性VOCs穿越亲水性细胞壁的途径及其潜在机制尚不明确。该研究系统观察并分析了薰衣草花、叶片及萌发种子胚上表皮毛的类型与分布；克隆获得了1个非特异性脂质转移蛋白基因（nsLTP2），并对其进行生物信息学分析、亚细胞定位研究；通过大肠杆菌（Escherichia coli）表达系统纯化nsLTP2蛋白，并利用荧光探针评估其与芳樟醇的结合能力。结果表明：薰衣草除盾状腺毛外，还具有2~3种形态各异的头状腺毛，二者在结构与分布上存在显著差异。nsLTP2基因编码由115个氨基酸组成的蛋白质，该蛋白具有典型的nsLTP蛋白结构域及8个高度保守的半胱氨酸残基。基因表达分析显示，nsLTP2基因主要在花中表达，且在初花期表达量最高。亚细胞定位分析表明，nsLTP2蛋白定位于细胞壁；体外试验进一步证实其具有结合脂质体的活性。综上所述，该研究证实nsLTP2蛋白是薰衣草疏水性VOCs转运途径中的关键组分，可促进VOCs在细胞壁中的运输过程。

关键词: 薰衣草, 腺毛, 非特异性脂质转移蛋白, 挥发性有机化合物, 细胞壁

Abstract:

The fragrance of lavender is primarily derived from volatile organic compounds（VOCs） secreted by the dense glandular trichomes on its surface. These hydrophobic VOCs must traverse the hydrophilic cell wall to be transported from their sites of synthesis to the secretory cavities of the trichomes for storage. However， the specific pathways and mechanisms enabling the efficient transport of hydrophobic VOCs across the hydrophilic cell wall in lavender remain poorly understood. In this study， we conducted a systematic analysis of the types and distribution of trichomes on the upper epidermis of flowers， leaves， and embryos of germinating lavender seeds. Additionally， a non-specific lipid transfer protein gene（nsLTP2） was cloned and subjected to bioinformatic and subcellular localization analyses. The nsLTP2 protein was expressed and purified using Escherichia coli， and its binding affinity for linalool， a major VOC component， was evaluated using a fluorescent probe. The findings revealed that， in addition to peltate glandular trichomes， lavender exhibited two to three morphologically distinct types of capitate glandular trichomes， which differred significantly in structure and distribution. The cloned nsLTP2 gene encoded a protein composed of 115 amino acids， featuring a typical nsLTP domain and eight highly conserved cysteine residues. Gene expression analysis demonstrated that the nsLTP2 gene was predominantly expressed in flowers， with peaked expression observed during the early flowering stage. Subcellular localization analysis indicated that nsLTP2 was localized to the cell wall， and in vitro assays confirmed its ability to bind liposomes. In summary， this study identified nsLTP2 as a critical component in the VOC transport pathway in lavender， facilitating the movement of VOCs through the cell wall.

Key words: lavender, glandular trichomes, nsLTP, volatile organic compounds, cell wall

中图分类号:

Q945.6

曾玉玲, 张义超, 马晓花, 杨琳, 聂静, 胡健健. 薰衣草腺毛非特异性脂质转移蛋白nsLTP2介导挥发性有机物的细胞壁转运[J]. 植物研究, 2026, 46(2): 220-230.

Yuling ZENG, Yichao ZHANG, Xiaohua MA, Lin YANG, Jing NIE, Jianjian HU. Non-specific Lipid Transfer Protein nsLTP2 Mediates the Cell Wall Transport of Volatile Organic Compounds in Lavender Glandular Trichomes[J]. Bulletin of Botanical Research, 2026, 46(2): 220-230.

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