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植物研究 ›› 2023, Vol. 43 ›› Issue (4): 572-581.doi: 10.7525/j.issn.1673-5102.2023.04.010

• 生理与生态 • 上一篇    下一篇

外源水杨酸对盐胁迫下珙桐幼苗抗氧化系统和基因表达的影响

徐磊, 胥晓, 刘沁松()   

  1. 西华师范大学生命科学学院,西南野生动植物资源保护教育部重点实验室,南充 637009
  • 收稿日期:2023-01-16 出版日期:2023-07-20 发布日期:2023-07-03
  • 通讯作者: 刘沁松 E-mail:qinsongliu@126.com
  • 作者简介:徐磊(1997—),男,硕士研究生,主要从事植物分子生物学研究。
  • 基金资助:
    国家自然科学基金项目(31900277);西华师范大学科研创新团队资金项目(KCXTD2022-4)

Effects of Exogenous Salicylic Acid on Antioxidant System and Gene Expression of Davidia involucrata Seedlings under Salt Stress

Lei XU, Xiao XU, Qinsong LIU()   

  1. College of Life Science,China West Normal University/Key Laboratory of Southwest China Wildlife Resources Conservation,Ministry of Education,Nanchong 637009
  • Received:2023-01-16 Online:2023-07-20 Published:2023-07-03
  • Contact: Qinsong LIU E-mail:qinsongliu@126.com
  • About author:XU Lei(1997—),male,master candidate,mainly engaged in plant molecular biology research.
  • Supported by:
    National Natural Science Foundation of China(31900277);Innovation Team Funds of China West Normal University(KCXTD2022-4)

摘要:

通过对珙桐(Davidia involucrata)幼苗进行水杨酸(SA)处理,探讨在盐胁迫下SA对珙桐叶片膜脂过 氧化程度、活性氧(ROS)积累、抗氧化酶活性、抗氧化剂含量等生理指标及基因表达的影响。结果表明:施用 2 mmol·L-1的SA可以显著降低盐胁迫下珙桐幼苗的相对电导率,抑制丙二醛(MDA)和ROS的积累,提高相对含水量、抗氧化酶(SOD、POD和APX)活性以及谷胱甘肽(GSH)含量。施用SA导致盐胁迫下2 581个珙桐基因表达发生变化,其中1 516个上调表达,1 065个下调表达。KEGG分析发现,差异表达基因富集在包括苯丙烷类生物合成在内的9条通路。此外,转录组与实时荧光定量PCR结果均显示,在盐胁迫条件下,转录因子基因DiWRKY40DiNAC25DiMYB4DiMYB86的表达受到SA的显著诱导。研究表明,外源SA可在盐胁迫下刺激珙桐幼苗产生逆境应答,引起基因表达发生变化,进而缓解盐胁迫对珙桐幼苗的伤害。

关键词: 珙桐, 水杨酸, 盐胁迫, 基因表达

Abstract:

To explore the effects of exogenous salicylic acid(SA)added on plants, the physiological indices including membrane lipid peroxidation, reactive oxygen species accumulation, antioxidant enzyme activities, and antioxidant contents as well as gene expression in the leaves of Davidia involucrata seedlings under salt stress were investigated respectively. The results revealed that, under salt stress, the application of SA significantly reduced the relative electrolyte leakage, and inhibited the accumulation of malondialdehyde(MDA)and ROS, and increased the relative water content, activities of antioxidant enzymes(i.e., SOD, POD, and APX), and glutathione(GSH)contents. Moreover, treatment with SA resulted in differential expression of 2 581 genes(1 516 up-regulated and 1 065 down-regulated) under salt stress. KEGG analysis indicated that differentially expressed genes(DEGs)were enriched in nine pathways including phenylpropanoid biosynthesis. In addition, transcriptome sequencing and quantitative real-time PCR(qRT-PCR)results showed that exogenous SA significantly induced the expression of transcription factor genes including DiWRKY40DiNAC25DiMYB4, and DiMYB86 under salt stress. These results suggested that exogenous SA might stimulate stress responses in salt-treated D. involucrata seedlings and trigger variation in gene expression, resulting in alleviation of salt injury in D. involucrata seedlings.

Key words: Davidia involucrata, salicylic acid, salt stress, gene expression

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