FaesAP2B基因在甜荞长雌蕊长雄蕊突变体lpls的表达分析

doi:10.7525/j.issn.1673-5102.2020.02.014

植物研究 ›› 2020, Vol. 40 ›› Issue (2): 266-273.doi: 10.7525/j.issn.1673-5102.2020.02.014

FaesAP2B基因在甜荞长雌蕊长雄蕊突变体lpls的表达分析

张娇, 王旋, 张良波, 刘志雄

长江大学园艺园林学院, 荆州 434025

收稿日期:2019-06-10 出版日期:2020-03-05 发布日期:2020-03-06
通讯作者: 刘志雄,E-mail:zxliu77@yahoo.com E-mail:zxliu77@yahoo.com
作者简介:张娇(1995-),女,硕士,主要从事园艺植物资源利用与研究。
基金资助:
国家自然科学基金项目（31571736；31771867）；国家公益性行业（农业）科研专项（201303008）；长江大学大学生创新训练项目（2018219）

Expression Analysis of the FaesAP2B in Mutant Buckwheat with Long Pistil and Long Stamen

ZHANG Jiao, WANG Xuan, ZHANG Liang-Bo, LIU Zhi-Xiong

College of Horticulture and Gardening, Yangtze University, Jingzhou 434025

Received:2019-06-10 Online:2020-03-05 Published:2020-03-06
Supported by:
Under the auspices of the National Natural Science Foundation of China(31571736;31771867);The Special Fund for Agro-scientific Research in the Public Interest of China(201303008);Innovation Training Program for Undergraduates of Yangtze University(2018219)

摘要/Abstract

摘要： 为弄清甜荞（Fagopyrum esculentum Moench.）长雌蕊长雄蕊突变体lpls花和籽粒发育调控的分子机制，从甜荞中克隆出1个长1 788 bp的AP2同源基因的cDNA序列，命名为FaesAP2B（GenBank登录号为MK290847.1）。序列结构分析表明：FaesAP2B基因包含1个长1 380 bp的完整开放阅读框（Open Reading Frame，ORF），编码1个由459个氨基酸残基组成的AP2/ERF家族转录因子，该转录因子含有2个高度保守的AP2结构域，第1个AP2结构域前还存在1个由10个氨基酸残基组成的核定位信号区。用qPCR检测FaesAP2B基因在甜荞lpls突变体根、茎、幼叶、花被片、雄蕊、雌蕊以及发育4 d的果实共7种器官中表达的组织特异性显示：FaesAP2B在甜荞突变体lpls营养组织和生殖结构中均有表达，但其在花器官和果实等生殖结构中的表达量明显高于营养组织，且在雄蕊中的表达量最高，极显著高于其在其他6种组织中的表达量（LSD，P<0.01），同时，FaesAP2B在花被片、雌蕊和发育4 d的果实中的表达量均极显著高于其在根、茎和叶等营养器官中的表达量（LSD，P<0.01），但该基因在其根、茎、叶间的表达量无显著性差异。推测该基因可能主要参与调控甜荞lpls突变体花和果实的发育。

关键词: 甜荞, 花发育, FaesAP2B, AP2

Abstract: In order to uncover the molecular mechanism of flower and grain development of buckwheat(Fagopyrum esculentum Moench.) with long pistil and long stamen mutant ‘lpls’ of a 1 788 bp long AP2 homologous cDNA sequence was cloned from F.esculentum Moench. and designated as FaesAP2B(GenBank accession number is MK290847.1). By sequence analysis, FaesAP2B gene contains a full Open Reading Frame with 1 380 bp long, and encodes an AP2/ERF family transcription factor consisting of 459 amino acid residues. The FaesAP2B transcription factor contains two highly conserved AP2 domains, with a nuclear localization signal region consisting of ten amino acid residues lying before the first AP2 domain. qPCR was performed to detect FaesAP2B gene expression in root, stem, leaf, perianth, stamen, pistil and juvenile fruit in four-day buckwheat mutant lpls, respectively. The FaesAP2B is expressed in both vegetative tissues and reproductive structures. However, the expression level of FaesAP2B in reproductive organs such as floral organs and fruits is significantly higher than that in vegetative tissues. In addition, the expression level of FaesAP2B in stamens is the highest among seven different organs(LSD, P<0.01). And the expression level of FaesAP2B in the perianth, pistil and four-day fruit was significantly higher than that in the vegetative organs such as roots, stems and leaves(LSD, P<0.01), but there was no significant difference in the expression of the gene between roots, stems and leaves. Our data suggested that FaesAP2B may be mainly involved in regulating flower and fruit development in the buckwheat mutant ‘lpls’.

Key words: Fagopyrum esculentum Moench., flower development, FaesAP2B, AP2

中图分类号:

S517

张娇, 王旋, 张良波, 刘志雄. FaesAP2B基因在甜荞长雌蕊长雄蕊突变体lpls的表达分析[J]. 植物研究, 2020, 40(2): 266-273.

ZHANG Jiao, WANG Xuan, ZHANG Liang-Bo, LIU Zhi-Xiong. Expression Analysis of the FaesAP2B in Mutant Buckwheat with Long Pistil and Long Stamen[J]. Bulletin of Botanical Research, 2020, 40(2): 266-273.

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FaesAP2B基因在甜荞长雌蕊长雄蕊突变体lpls的表达分析

Expression Analysis of the FaesAP2B in Mutant Buckwheat with Long Pistil and Long Stamen

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