濒危珍稀植物半枫荷的转录组分析

doi:10.7525/j.issn.1673-5102.2019.02.014

摘要/Abstract

摘要： 为加强中国特有濒危植物半枫荷资源的保护与利用工作，采用高通量测序平台Illumina HiSeq 2500对其进行转录组测序，将得到的数据过滤后进行de novo组装并聚类去冗余，获得77 629个Unigenes，通过九大功能数据库比对、分析、注释，最终有45 293个Unigenes获得注释信息；其中在KOG按功能分为25个子类，获得25 253个功能注释信息；GO功能注释可分为细胞组分、生物学过程、分子功能3大类、分别可细分为22、26、17亚类（共65个亚类）；与KEGG数据库对比，共发现286条代谢通路，其中发现可能与半枫荷药用活性成分相关的次生代谢产物生物合成的177条途径；根据组装结果预测出88个基因家族共1 547个编码转录因子的Unigenes，发现控制药效合成的转录因子家族。另外，根据注释结果检测到12 579个SNP多态位点和预测出57 671个CDS位点。本研究首次对半枫荷转录组进行分析，为深入开展半枫荷分子生物学研究提供基础数据来源。

关键词: 半枫荷, 转录组分析, 基因注释, 药用活性成分, 代谢通路

Abstract: In order to strengthen the conservation and utilization of China's endangered plant, Semiliquidambar cathayensis, a high-throughput sequencing platform, Illumina HiSeq 2500, was used to sequence the transcriptome of the plants. The obtained data was filtered and assembled by de novo. Then, 77 629 unigenes were obtained through classification and redundancy removal. By comparison analysis, annotation of the 9 functional databases, 45 293 unigenes get annotation information, which was divided into 25 sub-categories by function in KOG with 25 253 functional annotation information obtained. The functional annotation of GO could be divided into such three parts as cell components, biological processes, and molecular functions, which is composed of 2 226, and 17 subclasses respectively (65 subclasses in total). With the comparison of KEGG database, 286 metabolic pathways are found, among which there are 177 pathways to biosynthesis of secondary metabolites, which may be related to the medicinal active components of S.cathayensis. Based on the assembling result, 1 547 unigenes encoding the transcription factors are predicted in 88 gene families. Thus, a family of transcription factors controlling pharmacokinetic synthesis are discovered. Based on the annotation results of S.cathayensis, 12 579 SNP polymorphic sites are detected and 57 671 CDS sites are predicted. This study is the first time to analyze the transcriptional sequences of S.cathayensis, providing a basic data for the in-depth study on molecular biology in S.cathayensis.

Key words: Semiliquidambar cathayensis, transcriptome analysis, gene annotation, medicinal active ingredient, metabolic pathways

中图分类号:

Q949.751.4

叶兴状, 刘丹, 罗佳佳, 范辉华, 张国防, 刘宝, 陈世品. 濒危珍稀植物半枫荷的转录组分析[J]. 植物研究, 2019, 39(2): 276-286.

YE Xing-Zhuang, LIU Dan, LUO Jia-Jia, FAN Hui-Hua, ZHANG Guo-Fang, LIU Bao, CHEN Shi-Pin. Transcriptome Analysis for Rare and Endangered Plants of Semiliquidambar cathayensis[J]. Bulletin of Botanical Research, 2019, 39(2): 276-286.

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