YFT1基因突变影响番茄果色和硬度

doi:10.7525/j.issn.1673-5102.2018.05.020

植物研究 ›› 2018, Vol. 38 ›› Issue (5): 790-794.doi: 10.7525/j.issn.1673-5102.2018.05.020

YFT1基因突变影响番茄果色和硬度

李玲¹, 冯学超¹, 赵凌侠^1,2

1. 上海交通大学农业与生物学院番茄联合研究中心, 上海 200240;
2. 上海交通大学植物生物技术研究中心, 上海 200240

收稿日期:2018-01-04 出版日期:2018-09-15 发布日期:2018-08-31
通讯作者: 赵凌侠 E-mail:lxzhao@sjtu.edu.cn
作者简介:李玲(1988-),女,硕士研究生,主要从事番茄品质形成研究。
基金资助:
国家重点研发计划（2016YFD0100204-28）；上海市科技计划项目（16391900900）

Effect of Genetic Lesion of YFT1 on the Fruit Color and Firmness in Tomato

LI Ling¹, FENG Xue-Chao¹, ZHAO Ling-Xia^1,2

1. Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240;
2. Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240

Received:2018-01-04 Online:2018-09-15 Published:2018-08-31
Supported by:
National Key Research and Development Project(2016YFD0100204-28);The Key Technology Research and Development Program of Shanghai Technology Committee(16391900900)

摘要/Abstract

摘要： 番茄（Solanum lycopersicum）是全球第一大经济作物，其品质倍受科学家和消费者关注，果色和硬度是决定番茄品质的重要经济性状。为了解析YFT1（YELLOW FRUIT TOMATO1）调控番茄果色发育和硬度形成机制，本文对野生型cv.M82和突变体yft1的果色、硬度和果皮显微结构进行了比较分析。结果显示，在35 dpa（days post anthesis），yft1和cv.M82番茄果色、硬度、果皮细胞大小和形状无明显差异。随着果实发育cv.M82番茄由绿色转为橙色/浅红（47 dpa）和红色（54 dpa）；并由硬变软，54 dpa的果实硬度（13.68±1.78N）仅相当于35 dpa的（35.51±1.09N）1/3。同时，从转色期（47 dpa）果皮细胞由内向外逐渐变大；内果皮薄壁细胞由圆形（35 dpa）依次变成卵形（47 dpa）和不规则形（54 dpa），并观察到了细胞内陷和胞间空隙消失（54 dpa）。与cv.M82不同，yft1番茄的果色、硬度和果皮细胞显微结构随果实发育（35~54 dpa）变化不明显；而其果实硬度（47~54 dpa）显著高于cv.M82。这些结果均暗示YFT1突变使番茄果实发育延迟，并影响了果色发育和硬度的形成。该研究将为番茄果色发育和果色形成机制的揭示提供重要的表型证据。

关键词: yft1, 果色, 硬度, 表型

Abstract: Tomato(Solanum lycopersicum) is the biggest economic crop in the world, the quality was extensively concerned by customers and scientists, and the fruit colors and firmness are important economic traits to determine tomato quality. To dissect the mechanism of fruit color development and firmness formation regulated by YFT1(YELLOW FRUIT TOMATO1), the differences of the fruit colors, firmness and microstructure were comparatively analyzed between mutant of yellow fruit tomato 1(yft1) and wild type(WT, cv.M82). The differences derived from fruit color, firmness and size and shape of pericarps cells were not found between yft1 and cv.M82 on the 35 days post anthesis(dpa). However, fruits turned into orange/pale red(47 dpa) and full red(54 dpa) from green(35 dpa) with fruit development, and fruit gradually turned into softening, and fruit firmness at 54 dpa(13.68±1.78 N) was only equal to one third at 35 dpa(35.51±1.09 N). Meanwhile, the size of pericarp cells was increasing with fruit development from 47 dpa, and from inner to outer. We also found that shape of the inner pericarp cell changed into oval(47 dpa) and irregular shape(54 dpa) from globular shape(35 dpa), moreover observed invagination of cells and disappearance of the space between cells on the 54 dpa. In contrast with cv.M82, no obvious differences were found for fruit color, firmness, size and shape of pericarp cells in mutant yft1 during 35 dpa to 54 dpa. However, the fruit firmness in yft1 tomato was significantly higher than that in cv. M82. The results implied that genetic lesion of YFT1 delayed tomato fruit development, thus influenced both of fruit color development and firmness formation. The present research would provide important evidence at phenotypes level to reveal the molecular mechanisms of formation of fruit color and firmness.

Key words: yft1, fruit color, firmness, phenotype

中图分类号:

S641.2

李玲, 冯学超, 赵凌侠. YFT1基因突变影响番茄果色和硬度[J]. 植物研究, 2018, 38(5): 790-794.

LI Ling, FENG Xue-Chao, ZHAO Ling-Xia. Effect of Genetic Lesion of YFT1 on the Fruit Color and Firmness in Tomato[J]. Bulletin of Botanical Research, 2018, 38(5): 790-794.

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YFT1基因突变影响番茄果色和硬度

Effect of Genetic Lesion of YFT1 on the Fruit Color and Firmness in Tomato

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