鼠尾草叶绿体基因组序列分析

doi:10.7525/j.issn.1673-5102.2017.04.013

植物研究 ›› 2017, Vol. 37 ›› Issue (4): 572-578.doi: 10.7525/j.issn.1673-5102.2017.04.013

鼠尾草叶绿体基因组序列分析

何懿菡^1,2, 韩立敏³, 刘玉萍², 田娜¹, 苏旭², 王喆之¹

1. 陕西师范大学生命科学学院, 药用资源与天然药物化学教育部重点实验室, 西北濒危药材资源开发国家工程实验室, 西安 710119;
2. 青海师范大学生命与地理科学学院, 西宁 810008;
3. 陕西学前师范学院生命科学与食品工程学院, 西安 710061

收稿日期:2017-03-06 出版日期:2017-07-15 发布日期:2017-07-22
通讯作者: 王喆之,E-mail:zhezhiwang@outlook.com E-mail:zhezhiwang@outlook.com
作者简介:何懿菡(1987-),女,博士研究生,主要研究方向为药用植物次生代谢及分子调控。
基金资助:
国家自然科学基金项目（32170338）资助；陕西学前师范学院自然科学基金重点项目（2015ZDKJ005）资助

Complete Sequence Analysis of Chloroplast Genome of Salvia japonica

HE Yi-Han^1,2, HAN Li-Min³, LIU Yu-Ping², TIAN Na¹, SU Xu², WANG Zhe-Zhi¹

1. Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an 710119;
2. College of Geography and Life Science, Qinghai Normal University, Xining 810008;
3. College of Life Sciences and Food Engineering, Shaanxi Xueqian Normal University, Xi'an 710061

Received:2017-03-06 Online:2017-07-15 Published:2017-07-22
Supported by:
Under the auspices of the National Natural Science Foundation of China(32170338);The Key Project of Natural Science Foundation of Shaanxi Preschool Normal College(2015ZDKJ005)

摘要/Abstract

摘要： 鼠尾草（Salvia japonica）是唇形科（Labiatae）鼠尾草属（Salvia）的一种多年生草本植物，具有十分重要的药用和经济价值。本文采用第二代测序技术Illumina Hiseq平台对鼠尾草的叶绿体基因组进行测序，同时以鼠尾草近缘物种丹参叶绿体基因组作为参考，组装得到完整叶绿体基因组序列。结果表明，鼠尾草叶绿体基因组序列全长153 995 bp，呈典型的四段式结构，其中LSC区长84 573 bp，SSC区长19 874 bp，两个IR区分别长24 774 bp；鼠尾草叶绿体基因组成功注释13组叶绿体基因，基因的种类、数目及GC含量等与唇形科中其它物种较为类似。这些研究结果丰富了鼠尾草属的叶绿体基因组数据，为今后鼠尾草属植物系统发育关系重建积累了基础性数据。

关键词: 鼠尾草属, 鼠尾草, 叶绿体基因组, 基因结构, 系统进化

Abstract: Salvia japonica is a perennial herb of the genus Salvia(Lamiaceae), which is of great medicinal and economic value. The chloroplast genome of S.japonica was sequenced using Illumina Hiseq platform. The complete chloroplast genome sequence was obtained by Salvia miltiorrhiza chloroplast genome as a reference. The full-length chloroplast genome of S.japonica was 153 995 bp. The LSC was 84 573 bp and 19 874 bp in SSC, and the length of IR region was 24 774 bp. The chloroplast genome of S.japonica successfully annotated 13 groups of genes. The gene types, number and GC content were similar with other species of Lamiaceae. These results enrich the chloroplast data and also provide some valuable molecular data for reconstructing evolutionary history of Salvia.

Key words: Salvia, Salvia japonica, Chloroplast genome, gene structure, phylogenetics and evolution

中图分类号:

Q949.777.6

何懿菡, 韩立敏, 刘玉萍, 田娜, 苏旭, 王喆之. 鼠尾草叶绿体基因组序列分析[J]. 植物研究, 2017, 37(4): 572-578.

HE Yi-Han, HAN Li-Min, LIU Yu-Ping, TIAN Na, SU Xu, WANG Zhe-Zhi. Complete Sequence Analysis of Chloroplast Genome of Salvia japonica[J]. Bulletin of Botanical Research, 2017, 37(4): 572-578.

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鼠尾草叶绿体基因组序列分析

Complete Sequence Analysis of Chloroplast Genome of Salvia japonica

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