空气凤梨叶片蜡质成分及叶表结构研究

doi:10.7525/j.issn.1673-5102.2016.05.009

植物研究 ›› 2016, Vol. 36 ›› Issue (5): 692-696.doi: 10.7525/j.issn.1673-5102.2016.05.009

空气凤梨叶片蜡质成分及叶表结构研究

郑桂灵, 张伟, 李鹏

青岛农业大学资源与环境学院, 青岛 266109

收稿日期:2016-05-04 出版日期:2016-09-15 发布日期:2016-09-27
通讯作者: 李鹏,E-mail:pengleep@163.com E-mail:pengleep@163.com
作者简介:郑桂灵(1980-),女,博士,主要从事植物学研究。
基金资助:
青岛市科技计划项目（14-2-4-46-jch）；国家自然科学基金项目（41475132，41571472）；青岛农业大学高层次人才基金项目（1113351，1115002）

Leaf Wax Components and Surface Structure of Tillandsia Species

ZHENG Gui-Ling, ZHANG Wei, LI Peng

College of Resource and Environment, Qingdao Agricultural University, Qingdao 266109

Received:2016-05-04 Online:2016-09-15 Published:2016-09-27
Supported by:
Qingdao Science and Technology Program(14-2-4-46-jch);National Natural Science Foundation of China(41475132,41571472);Scientific Foundation of Qingdao Agricultural University(1113351,1115002)

摘要/Abstract

摘要： 空气凤梨是一类依靠叶片吸收水分和养分的特殊植物类群。为进一步探讨其叶片功能，以五种空气凤梨为材料，通过扫描电镜（SEM）和气相色谱-质谱联用法（GC-MS）分析了其叶表结构和蜡质成分。结果表明空气凤梨叶表面覆盖有白色、蜡质、葵花状的鳞片，由翼状细胞、环状细胞和碟状细胞三种细胞构成。叶片蜡质成分中以低极性的烃、醇、酮、酯等物质为主，可起到有效减少水分散失的作用。对五种空气凤梨叶片蜡质成分总含量分析表明，小精灵空气凤梨 >贝克利空气凤梨 >维路提拉空气凤梨 >女王头空气凤梨 >电卷烫空气凤梨。而相关性分析则表明，蜡质成分总含量与空气凤梨鳞片总面积呈显著的正相关，暗示空气凤梨叶片蜡质成分主要存在于叶表的鳞片上。

关键词: 空气植物, 叶表鳞片, 气相色谱-质谱联用, 蜡质成分

Abstract: Tillandsia is a kind of special plant which absorbs water and nutrients directly from the air through leaves. In order to investigate the function of the leave, five Tillandsia species were chose to analyze their leave surface structures with scanning electron microscope(SEM) and wax components with gas chromatography-mass spectrometry technology(GC-MS). The white, waxy, sunflower-shaped trichomes covered the leaf surface in all species. The foliar trichomes were made up of disc cells, ring cells and wing cells. The main wax components contained low-polarity constituents, including hydrocarbons, alcohols, ketones and esters, which may reduce the water loss effectively. In addition, the total contents of wax components among five Tillandsia species were different with the order of Tillandsia ionantha >Tillandsia brachycaulos >Tillandsia velutina >Tillandsia medusa >Tillandsia streptophylla. By the correlation analysis, the total contents of wax components were positively correlated with the total leave area of foliar trichomes, indicating that those wax components were distributed mainly on the foliar trichomes.

Key words: air plant, foliar trichomes, GC-MS, wax components

中图分类号:

S668.3

郑桂灵, 张伟, 李鹏. 空气凤梨叶片蜡质成分及叶表结构研究[J]. 植物研究, 2016, 36(5): 692-696.

ZHENG Gui-Ling, ZHANG Wei, LI Peng. Leaf Wax Components and Surface Structure of Tillandsia Species[J]. Bulletin of Botanical Research, 2016, 36(5): 692-696.

参考文献

1. 宋超,王婧,郭彦军,等.植物表皮蜡质对环境胁迫的响应[J].植物生理学报,2011,47(10):951-956.Song C,Wang J,Guo Y J,et al.Response of plant epicuticular wax to environmental factors[J].Plant Physiology Journal,2011,47(10):951-956.
2. Riederer M,Schreiber L.Protecting against water loss:analysis of the barrier properties of plant cuticles[J].Journal of Experimental Botany,2001,52(363):2023-2032.
3. 曾琼,刘德春,刘勇.植物角质层蜡质的化学组成研究综述[J].生态学报,2013,33(17):5133-5140.Zeng Q,Liu D C,Liu Y.The overview and prospect of chemical composition of plant cuticular wax[J].Acta Ecologica Sinica,2013,33(17):5133-5140.
4. Kosma D K,Bourdenx B,Bernard A,et al.The impact of water deficiency on leaf cuticle lipids of Arabidopsis[J].Plant Physiology,2009,151(4):1918-1929.
5. Benzing D H,Bennett B,Brown G,et al.Bromeliaceae:profile of an adaptive radiation[D].Cambridge:Cambridge University Press,2000.
6. 王思维,郑桂灵,付英,等.气生凤梨叶片结构研究[J].植物研究,2010,30(2):140-145.Wang S W,Zheng G L,Fu Y,et al.Foliar structure of several species of epiphytic Tillandsia(Bromeliaceae)[J].Bulletin of Botanical Research,2010,30(2):140-145.
7. Martin C E.Physiological ecology of the Bromeliaceae[J].The Botanical Review,1994,60(1):1-82.
8. Calasans C F,Malm O.Elemental mercury contamination survey in a chlor-alkali plant by the use of transplanted Spanish moss,Tillandsia usneoides L.[J].Science of the Total Environment,1997,208(3):165-177.
9. 李俊霖,李鹏,王恒蓉,等.特殊植物类群空气凤梨对大气污染物甲醛的净化[J].环境工程学报,2013,7(4):1451-1458.Li J L,Li P,Wang H R,et al.Purification of air pollutant formaldehyde with special plant group epiphytic Tillandsia[J].Chinese Journal of Environmental Engineering,2013,7(4):1451-1458.
10. Bauer S,Schulte E,Thier H P.Composition of the surface waxes from bell pepper and eggplant[J].European Food Research and Technology,2005,220(1):5-10.
11. Van Maarseveen C,Jetter R.Composition of the epicuticular and intracuticular wax layers on Kalanchoe daigremontiana(Hamet et Perr.de la Bathie) leaves[J].Phytochemistry,2009,70(7):899-906.
12. 王绍辉,张福墁.黄瓜叶表面特性与生态适应性[J].生态学报,2003,23(1):199-204.Wang S H,Zhang F M.Studies on relationship between the ecophysiological feature and leaf characteri stics of cucumber[J].Acta Ecologica Sinica,2003,23(1):199-204.
13. Pierce S,Maxwell K,Griffiths H,et al.Hydrophobic trichome layers and epicuticular wax powders in Bromeliaceae[J].American Journal of Botany,2001,88(8):1371-1389.
14. 高爱丽,赵秀梅,秦鑫.水分胁迫下小麦叶片渗透调节与抗旱性的关系[J].西北植物学报,1991,11(1):58-63.Gao A L,Zhao X M,Qin X.Relationship between osmotic adjustment and drought resistance in wheat under water stress[J].Acta Botanica Boreali-Occidentalia Sinica,1991,11(1):58-63.
15. 李德全,邹琦,程炳嵩.冬麦叶片的渗透调节能力与产量及抗旱性的关系[J].植物学报,1996,13(增刊):46-50.Li D Q,Zhou Q,Cheng B H.Osmotic adjustment ability of winter wheat leaves and relation to yield and drought resistance[J].Chinese Bulletin of Botany,1996,13(zk):46-50.
16. 郭彦军,倪郁,郭芸江,等.空气湿度与土壤水分胁迫对紫花苜蓿叶表皮蜡质特性的影响[J].生态学报,2011,31(18):5273-5280.Guo Y J,Ni Y,Guo Y J,et al.Effects of air humidity and soil water deficit on characteristics of leaf cuticular waxes in alfalfa(Medicago staiva)[J].Acta Ecologica Sinica,2011,31(18):5273-5280.
17. Oliveira A F M,Meirelles S T,Salatino A.Epicuticular waxes from caatinga and cerrado species and their efficiency against water loss[J].Anais da Academia Brasileira de Ciências,2003,75:431-439.
18. Kolattukudy P E.Plant waxes[J].Lipids,1970,5(2):259-275.

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空气凤梨叶片蜡质成分及叶表结构研究

Leaf Wax Components and Surface Structure of Tillandsia Species

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