外源硝态氮水平对长春花生理代谢的影响

doi:10.7525/j.issn.1673-5102.2016.04.008

植物研究 ›› 2016, Vol. 36 ›› Issue (4): 535-541.doi: 10.7525/j.issn.1673-5102.2016.04.008

外源硝态氮水平对长春花生理代谢的影响

朱孟炎, 于博帆, 陈华峰

东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040

收稿日期:2016-01-15 出版日期:2016-07-15 发布日期:2016-06-15
通讯作者: 陈华峰,E-mail:biginsect2002@163.com E-mail:biginsect2002@163.com
作者简介:朱孟炎(1991-),女,硕士研究生,主要从事植物生理生态学研究工作。
基金资助:
国家自然科学基金青年科学基金项目(31400337)

Effects of Nitrate Nitrogen Levels on Physiological Metabolism of Catharanthus roseus

ZHU Meng-Yan, YU Bo-Fan, CHEN Hua-Feng

Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040

Received:2016-01-15 Online:2016-07-15 Published:2016-06-15
Supported by:
National Natural Science Foundation of Youth Science Foundation(31400337)

摘要/Abstract

摘要： 以长春花为实验材料,采用珍珠岩为培养基质,设置不同氮素浓度,对其各器官的初生和次生代谢进行了初步研究,结果如下:植株中3种主要生物碱含量均是随着外源氮水平的降低而降低,外源硝态氮水平对长春花文朵灵和长春质碱在不同叶位叶片中的积累特点影响较明显,而对长春碱的积累影响较小。在高氮条件下,植物体内合成和积累较高水平的游离氨基酸,可能为部分生物碱的合成提供了骨架,并能促进生物碱含量的提高。蔗糖含量在不同叶位叶片中也是随着叶龄的增加而缓慢增加。同时,高氮水平下,叶片中蔗糖含量明显高于正常供氮水平下的含量。本研究为提高长春花中具有重要药用价值生物碱的含量提供参考依据。

关键词: 长春花, 硝态氮, 物碱, 次生代谢

Abstract: With Catharanthus roseus, we used perlite as culture substrate to study the biomass and secondary metabolism. Three main alkaloids in plants were decreased with reduced levels of nitrogen. The level of exogenous nitrate on vindoline and Catharanthine in C.roseus was with more significant accumulation in the leaves at different leaf positions, but with little effect on the accumulation of vinblastine. In high nitrogen condition, the plant synthesis and accumulation were at higher levels of free amino acids, which may provide the skeleton and promote the improvement of alkaloids in the partial synthesis. The content of sucrose in different position leaves was increased slowly with the increase of age. The content of sucrose in leaf at higher nitrogen level was significantly higher than that at normal nitrogen level. This research provide the reference basis that the important medicinal value for improving the content of alkaloid.

Key words: Catharanthus roseus, nitrate nitrogen, alkaloid, secondary metabolism

中图分类号:

S143.1

朱孟炎, 于博帆, 陈华峰. 外源硝态氮水平对长春花生理代谢的影响[J]. 植物研究, 2016, 36(4): 535-541.

ZHU Meng-Yan, YU Bo-Fan, CHEN Hua-Feng. Effects of Nitrate Nitrogen Levels on Physiological Metabolism of Catharanthus roseus[J]. Bulletin of Botanical Research, 2016, 36(4): 535-541.

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外源硝态氮水平对长春花生理代谢的影响

Effects of Nitrate Nitrogen Levels on Physiological Metabolism of Catharanthus roseus

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