关键词: 黑穗醋栗(Ribes nigrum L.), 未受精胚珠, 离体培养, 体细胞胚状体, 再生植株

Abstract: This paper first reported that Baopiheidou, one of three cultivarsin this experiment was able to produce adventive embryos when theunfertilized ovules of black currant (Ribes nigrum L.) were inoculated onMS medium supplemented with plant hormones BA, 2.4-D, GA₃.Thesomatic embyogenic calli were inductedo in basal medium MS supplementedwith 5.0-3.0mg l^-1 BA, 2.0mg l^-1 2.4-D or 1.0mg l^-1 GA₃. After cultu-ring the unfertilized ovules about 25 days on the induction medium, the calli were trasfered to the embryo formation medium for the embryodevelopment. The adventive embryos were observed after culturing about20 days on the embryo formation medium (Basal medium MS+3.0mg l^-1BA+1.0mg l^-1 GA₃+3% sucrose.). Somatic embryo with 0.5-1.0 cmlength was able to form whole plantlet in medium MS+2.0mg l^-1 IBA +2.0mg l^-1 GA₃+1% sucrose. The experiments showed:The variety andinduction stage of inoculated ovules affected the somatic embryogenesis.The somatic embryogenesis was inducted when ovules at the uninuleatestage were inoculated. Its induction frequency was 3.3%-5.0%. BA in themedium was necessary for the induction of somatic embryogenesis andembryo development. We also had established somatic embryogenic callusclone directly from somatic cell of black currant cultivar and maintainnedmore than two years with the avaibility of embryogenesis, It can be usedas a favourable material for the micropropagation, mutation selection ofcell suspension culture, protoplast isolation and culture and genetic manipulation in black currant.

Key words: Black currant(Ribes nigrun L.), Unertilized ovules, In vitro culture, Somatic embryo, Plantlet regeneration