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植物研究 ›› 1995, Vol. 15 ›› Issue (4): 477-484.

• 论文 • 上一篇    下一篇

应用酯酶同工酶对甘草属长荚果系的不同种亲缘关系的研究

潘鼎元, 张煜星, 李学禹   

  1. 新疆石河子农学院, 新疆, 石河子市 832003
  • 收稿日期:1995-03-01 出版日期:1995-12-15 发布日期:2016-06-14

A STUDY ON RELATIONSHIP OF DIFFERENT SPECIES OF Glycyrrhiza Longilegumata THROUGY ESTERASE-ISOZYMES

Pan Ding-yuan, Zhang Yu-xing, Li Xue-yu   

  1. Xinjiang Shihezi Agricultural College, Shihezi 832003
  • Received:1995-03-01 Online:1995-12-15 Published:2016-06-14

摘要: 对甘草属的长荚果系, 采取聚丙稀酰胺凝胶电泳法, 分析它们的酯酶同工酶。结果表明:每个种具有明显的酶谱差异, 有其特征酶带;从酶谱的相似度指数来看, 6.6a、8、8a的相似度指数较高, 亲缘关系较近。而2、4、5、8b的相似性度指数较低, 它们之间的亲缘关系较远。经过簿层色谱扫描, 根据各酶谱的峰面积的百分率, 用系统聚类法, 最短距离法进行聚类分析, 以欧氏距离4.0为分类线, 把甘草属长荚果系分为二类, Ⅰ类:1、5、6、6a、7、8、8b;Ⅱ类2a、4、8a、3、2.

关键词: 甘草属, 酯酶同工酶, 亲缘关系

Abstract: The Esteras-isozymes of Glycyrrhiza Longilegumata X. Y. Li have been comparatively studied by means of polyarcylamide gel electrophoresis. The interspecific zymogramatic differences are obvious. Each species possesses its specific zymograms, distinguishable from the others. By means of the zymogramatic index of similarity these species was analysed. It shows the close affinity among 6. 6a、8、8a there is high index of similarity, but they are very far from 2, 4, 5, 8b on the basis of percentage of each zymogramatic peak area with the scanning chromatograms. By means of the systematic cluster and the short distance were caster analysis. When the Euclid's distance was higher than 4. 0 or lower than 4, OGlycyrrhi-za Longilegumata X. Y. Li may be grouped in two categories. 1,5,6,6a,7,8,8b into one category, the seconed category conzains'v-:-a, 3,2.

Key words: Glycyrrhiza, Esterse-isozyme, Affinity