毒源烟草试管保存技术的研究

植物研究 ›› 2002, Vol. 22 ›› Issue (3): 333-336.

毒源烟草试管保存技术的研究

郭志平

黑龙江省克山师专生物系, 克山 161601

收稿日期:2001-05-21 出版日期:2002-09-15 发布日期:2016-06-14
作者简介:郭志平(1965-),男,副教授。主要从事马铃薯栽培和马铃薯病毒方面的研究。

RESEARCH OF TUBE STORAGE TECHNIQUE AND ITS EFFECTS OF TABACCO CONTAINED VIRUSES

GUO Zhi-ping

Biology Department, Keshan Teachers College, Keshan 161601

Received:2001-05-21 Online:2002-09-15 Published:2016-06-14

摘要/Abstract

摘要： 经三年连续试验,用试管保存含马铃薯X病毒(PVX)、马铃薯Y病毒(PVY)、烟草花叶病毒(TMV)的毒源烟草,结果证明毒源烟草可在试管中正常生长发育。负染法和免疫电镜捕获法检测结果证明,试管毒源烟草不同生育期,体内的病毒含量不同;试管毒源烟草在管外土壤中栽培可正常生长发育,并表现出相应的症状,接种指示植物试验证明其侵染力与温室内保存的毒源效果相同。

关键词: 试管保存, 毒源烟草, PVX、PVY、TMV病毒

Abstract: Through three-yesr's experiment of storing tabacco contained PVX. PVY. TMV viruses, a conclusion is arrived at that the tabacco can grow normally iy tube. It is suggested by counter-dry-transfer process and immunological electron microscope capture that the viruses content vary at the tabacco's various grouth stage in tube, and that tabacco can grow normally outside the tube, showing relevant symptoms. The experiment of inoculation indicates that its infectivity is equal to that of viruses stored inside.

Key words: tube storage, tabacco contained viruses, PVX. PVY.TMV viruses

郭志平. 毒源烟草试管保存技术的研究[J]. 植物研究, 2002, 22(3): 333-336.

GUO Zhi-ping. RESEARCH OF TUBE STORAGE TECHNIQUE AND ITS EFFECTS OF TABACCO CONTAINED VIRUSES[J]. Bulletin of Botanical Research, 2002, 22(3): 333-336.