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植物研究 ›› 2003, Vol. 23 ›› Issue (1): 51-54.

• 论文 • 上一篇    下一篇

沙葱(Allium mongolicum Regel)离体培养再生可育植株

陈刚, 贾敬芬, 郝建国   

  1. 西北大学生命科学学院, 西安 710069
  • 收稿日期:2002-07-03 出版日期:2003-03-15 发布日期:2016-06-14
  • 作者简介:陈刚(1974-),男,博士,主要从事植物生物技术研究。
  • 基金资助:
    国家自然科学基金(批准号30070366)资助

FERTILE PLANT REGENERATION FROM TISSUE CULTURE IN ALLIUM MONGOLICUM REGEL

CHEN Gang, JIA Jing-Fen, HAO Jian-Guo   

  1. Life Science School, Northwest University, Xi'an 710069
  • Received:2002-07-03 Online:2003-03-15 Published:2016-06-14

摘要: 以沙葱(Allium mongolicum Regel)的叶基切段作外植体,成功地诱导出愈伤组织和再生植株。诱导愈伤组织的培养基为附加2 mgL-1 2, 4-D和0.2 mgL-1 KT的MS培养基。愈伤组织转移到附加2 mgL-1 6BA和0.4 mgL-1 NAA的MS培养基后分化出大量的苗。分化苗在含有IBA 1 mgL-1的1/2MS培养基上生根最佳。再生植株移栽成活率在95%以上,移栽到田间的植株第二年以后可正常开花结实。

关键词: 沙葱, 组织培养, 植株再生

Abstract: A highly efficient regeneration system of Allium mongolicum Regel was established from leaf base segments via tissue culture. MS medium supplemented with 2 mgL-1 2, 4-D and 0.2 mgL-1 KT was suitable for callus induction. The shoots differentiated on MS medium added with 2 mgL-1 6BA and 0.4 mgL-1 NAA. The half strength MS medium containing 1 mgL-1 IBA was more effective for the inducing roots. The regenerated plantlets were transplanted to the soil, and over 95% of them were survival. At the second year after transplanting, the plantlets flowered and set seeds.

Key words: Allium mongolicum Regel, tissue culture, plant regeneration