关键词: 大花蕙兰, 原球茎, 组织培养, 生长指数

Abstract: By using KT medium which to be added different content of plant growth substance such as BA, KT, NAA and the combination of KT and NAA, we grant on the subculture of active green globlar body (GGB)of Cymbidium grandiflorum for stand and got these results:①BA can speed the propagation of GGB, but when the content was more than 0.5 mg/L, the propagation speed got down and the green globlar body became smaller and black green.②The propagation effect of KT was better than BA, but when the content of KT was more than 1.0 mg/L, the propagation speed got down, and GGB became smaller.③NAA was benefit to the propagation of GGB, the effect was obviously better than BA and KT, but when the content was more than 1.0 mg/L, the propagation speed didn't change, and GGB became brown partly.④under the combination of KT 0.5~0.7 mg/L and NAA 0.7~1.0 mg/L., the propagation speed and quality of GGB were ideal.Because the less content of plant growth substance have an advantage of the heredity stability.We choose the combination of KT 0.5 mg+NAA 0.7 mg/L.as the propagation medium of GGB.

Key words: Cymbidium grandiflorum, green globlar body, tissue culture, propagation coefficient