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植物研究 ›› 2004, Vol. 24 ›› Issue (3): 305-308.

• 论文 • 上一篇    下一篇

萝卜CMS不育系与保持系小孢子发生的细胞学研究

黄浩1,2, 柳李旺2, 陈崇顺1, 龚义勤2, 宋贤勇2, 韦开余2   

  1. 1. 南京师范大学生命科学学院, 南京 210097;
    2. 南京农业大学作物遗传与种质创新国家重点实验室, 南京农业大学园艺学院, 南京 210095
  • 收稿日期:2004-01-07 出版日期:2004-09-15 发布日期:2016-06-14
  • 通讯作者: 柳李旺,E-mail:nauliulw@njau.edu.cn E-mail:nauliulw@njau.edu.cn
  • 作者简介:黄浩(1977-), 硕士, 主要从事蔬菜作物遗传育种研究。
  • 基金资助:
    上海农业科技攻关重点项目(农科攻1-4), 江苏省高技术项目(BG99501-4), 南京农业大学SRT 项目(0303B04)

Cytological investigation on microsporogenesis of cytoplasmic male sterility in radish(Raphanus sativus L.)

HUANG Hao1,2, LIU Li-Wang2, CHEN Chong-Shun1, GONG Yi-Qin2, SONG Xian-Yong2, WEI Kai-Yu2   

  1. 1. College of Life science, Nanjing Normal University, Nanjing 210097;
    2. National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing 210095
  • Received:2004-01-07 Online:2004-09-15 Published:2016-06-14

摘要: 研究了萝卜胞质雄性不育系A2、A4及其相应保持系B2、B4的小孢子发生与花药壁发育的细胞学特征。结果表明,不育系A2的绒毡层细胞在四分体时期出现异常,小液泡增多,至单核期汇合形成大液泡,绒毡层细胞异常膨大;小孢子外壁染色浅,细胞壁受到破坏,最后与绒毡层一同降解。不育系A4在减数分裂期即表现出异常,绒毡层异常肥大;花药发育后期,小孢子外壁亦染色较浅;绒毡层细胞融合形成细胞团块侵入药室挤压小孢子,两者一同降解。

关键词: 萝卜, 胞质雄性不育, 小孢子发生, 细胞形态学

Abstract: The cytology of the microsporogenesis and development of anther wall of cytoplasmic male sterile (CMS) lines A2,A4 and its maintainer lines B2,B4 was studied.The results revealed that the tapetal cells of the male sterile line A2 developed abnormally at tetrad stage,and they became vacuolated and numerous small vacuoles are present;furthermore,the small vacuoles fused into large ones.Exine of microspore was light stained,and cell wall was destructed.Finally with degeneration of the tapetal cells,the pollen aborted.The tapetal cells of CMS line A4 enlarged abnormally at meiotic phase,pressing microsporocyte seriously.Lately,light-staining exine of microspore could not be observed,and tapetal cells fused to form big lump invading the loculi,and finally leading to pollen grain abortion.

Key words: Raphanus sativus, cytoplasmic male sterility, microsporogenesis, cytomorphology