三倍体芍药品种花粉母细胞减数分裂行为观察与比较

doi:10.7525/j.issn.1673-5102.2016.03.004

植物研究 ›› 2016, Vol. 36 ›› Issue (3): 341-347.doi: 10.7525/j.issn.1673-5102.2016.03.004

三倍体芍药品种花粉母细胞减数分裂行为观察与比较

王玉蛟¹, 刘建鑫¹, 于晓南^1,2,3

1. 北京林业大学园林学院, 北京 100083;
2. 国家花卉工程技术研究中心, 北京 100083;
3. 花卉种质资源创新与育种北京市重点实验室, 北京 100083

收稿日期:2015-11-23 出版日期:2016-05-15 发布日期:2016-05-20
通讯作者: 于晓南,E-mail:yuxiaonan626@126.com E-mail:yuxiaonan626@126.com
作者简介:王玉蛟(1993-),女,硕士研究生,主要从事芍药种质资源与育种的研究。
基金资助:
国家自然科学基金(31400591)

Meiosis Observation and Comparison of Pollen Mother Cells of Triploid Paeonia lactiflora Cultivars

WANG Yu-Jiao¹, LIU Jian-Xin¹, YU Xiao-Nan^1,2,3

1. Beijing Forestry University, Beijing 100803;
2. National Engineering Research Center for Floriculture, Beijing 100083;
3. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecule Breeding, Beijing 100083

Received:2015-11-23 Online:2016-05-15 Published:2016-05-20
Supported by:
Chinese Nature Science Foundation(31400591)

摘要/Abstract

摘要： 芍药是中国传统名花,我国品种均为二倍体,培育多倍体新品种是一项长期的育种目标。本研究选取3个芍药三倍体新品种,采用植物染色常规压片法观察其花粉母细胞的减数分裂情况。结果显示:(1)同一品种不同花蕾直径大小的花粉母细胞所处的分裂状态不同,同一品种同一花蕾直径大小花粉母细胞有不同步分裂现象,但大多数表现为相同的分裂相;(2)不同品种的相近花蕾直径大小所处的分裂时期也不同,有比较显著的差异,因此直径不能作为判断减数分裂时期的唯一的标准;(3)三倍体品种花粉母细胞减数分裂存在单价体、三价体、染色体桥、染色体不均等分离等染色体异常行为,这些异常行为使一些遗传物质缺失或最终形成二分体或三分体,导致三倍体花粉的育性非常低。该研究为掌握芍药三倍体减数分裂规律及多倍体育种奠定了细胞学基础。

关键词: 芍药, 三倍体, 减数分裂

Abstract: Paeonia lactiflora is a traditional Chinese flower. The traditional cultivars are diploid, and cultivating new cultivars has become an important goal in breeding. We selected three new cultivars of peony triploid, ‘Brightness', ‘Roseletter' and ‘Coral Sunset', and observed its meiosis behavior of pollen mother cells by fluorescence microscope. The pollen mother cells of the same variety with same bud diameter were not divided synchronously. But the majority performed same meiosis state. The different cultivars of the similar bud diameter were also different in the period of meiosis, and had significant differences. There were many chromosome abnormal behaviors in the meiosis of pollen mother cell of triploid cultivars, such as the univalent chromosome, the trivalent chromosome, the chromosome bridge and unequal separation and so on. These abnormal behavior leading to triploid pollen fertility is very low.

Key words: Paeonia lactiflora, triploid, meiosis

中图分类号:

S682.1+2

王玉蛟, 刘建鑫, 于晓南. 三倍体芍药品种花粉母细胞减数分裂行为观察与比较[J]. 植物研究, 2016, 36(3): 341-347.

WANG Yu-Jiao, LIU Jian-Xin, YU Xiao-Nan. Meiosis Observation and Comparison of Pollen Mother Cells of Triploid Paeonia lactiflora Cultivars[J]. Bulletin of Botanical Research, 2016, 36(3): 341-347.

参考文献

1. 于晓南,宋焕芝,郑黎文. 国外观赏芍药育种与应用及其启示[J].湖南农业大学学报:自然科学版,2010,36(2):159-162. Yu X N,Song H Z,Zheng L W. Brief introduction of breeding and horticulture utilization of ornamental herbaceous peony at abroad[J].Journal of Human Agriculture University:Natural Sciences,2010,36(2):159-162.
2. Yu X N,Guo P P,Lu G P,et al. Optimum harvesting time of herbaceous peony buds for cutting flowers[J].Journal of Forestry Research,2011,22(1):137-140.
3. Hong D Y,Pan K Y,Zhou S L,et al. Peonies of the world[M].Kew:Royal Botanic Gardens,2010.
4. 王历慧,于晓南,郑黎文. 中西方芍药切花应用与市场趋势分析[J].黑龙江农业科学,2011(2):147-149. Wang L H,Zheng L W,Yu X N. Analysis of applications and market trends of herbaceous peony cut flower in China and the West[J].Heilongjiang Agriculture and Sciences,2011(2):147-149.
5. 许重阳,王君,叶粟,等. 白杨杂种三倍体小孢子母细胞减数分裂及花粉形态观察[J].西北植物学报,2011,31(12):2454-2458. Xu C Y,Wang J,Ye S,et al. Meiosis of microsporocyte and variation of pollen in an allotriploid white poplar[J].Acta Bot Boreal Occident Sin,2011,31(12):2454-2458.
6. 戴思兰. 园林植物遗传学[M].北京:中国林业出版社,2010. Dai S L. Genetics of landscape plants[M].Beijing:China Forestry Publishing House,2010.
7. 陈瑶. 天然三倍体枇杷小孢子母细胞减数分裂研究[D].重庆:西南大学,2008. Chen Y. Studied on meiosis proeess of pollen mother Cells(PMC) of Natural Triploid Loquat[D].Chongqing:Southwest University,2008.
8. Qian C T,Jahn M M,Staub J E,et al. Meiotic chromosome behavior in an allotriploid derived from an amphidiploid × diploid mating in Cucumis[J].Plant Breeding,2005,124:272-276.
9. 许晓光,陈龙,彭建营. 酸枣和赞皇大枣花粉母细胞减数分裂的观察[J].园艺学报,2009. 36(8):1127-1133. Xu X G,Chen L,Peng J Y. Meiotic observations on the pollen mother cells of Ziziphus spinosus Hu. and Ziziphus jujuba Mill. ‘Zanhuang Dazao’[J].Acta Horticulturae Sinica,2009,36(8):1127-1133.
10. 熊治廷. 猕猴桃种间杂种三倍体形态学和减数分裂观察[J].植物研究,1990,10(1):99-103. Xiong Z T. Observations on the morphology and meiosis of interspecies hybrid triploid in Actinidia[J].Bulletin of Botanical Research,1990,10(1):99-103.
11. 崔娜新,陈发棣,赵宏波,等. 几种菊属植物及其种间杂种减数分裂行为观察[J].园艺学报,2006,33(5):1033-1038. Cui X N,Chen F D,Zhao H B et al. Research on meiosis of some Dendranthema species and their hybrids[J].Acta Horticulturae Sinica,2006,33(5),1033-1038.
12. Wang J,Kang X Y,Zhu Q. Variation in pollen formation and its cytological mechanism in an allotriploid white poplar[J].Tree Genetics & Genomes,2010,6:281-290.
13. 何丽霞. 黄牡丹花粉母细胞减数分裂过程的细胞遗传学观察[J].兰州大学学报:自然科学版,2004,40(6):78-82. He L X. Observation on meiosis of pollen mother cells of Paeonia delavayi in cytogenetics[J].Journal of Lanzhou University:Natural Sciences,2004,40(6):78-82.
14. 梁国鲁,李育农,李晓林. 中国苹果属植物小孢子减数分裂染色体系统研究[J].西南农业大学学报,1996,18(4):299-307. Liang G L,Li Y N,Li X L. Evolutionary study of the chromosomes at pollen mother cell meiosis in Malus[J].Journal of Southwest Agriculture University,1996,18(4):299-307.
15. 封紫,魏迟,贾桂霞. 异源三倍体百合减数分裂染色体行为的研究[J].北京林业大学学报,2012,34(2):118-124. Feng Z,Wei C,Jia G X. Meiotic chromosome behavior in allotriploid cultivar of Lilium[J].Journal of Beijing Forestry University,2012,34(2):118-124.
16. 康向阳,朱之悌,张志毅. 毛白杨异源三倍体形态和减数分裂观察[J].北京林业大学学报,1999,21(1):1-5. Kang X Y,Zhu Z T,Zhang Z Y. Morphology and meiosis of Chinese white poplar[J].Journal of Beijing Forestry University,1999,21(1):1-5.
17. 申书兴,李振秋,张成合,等. 大白菜3号6号染色体双三体及其初级三体的鉴定[J].园艺学报,2002,29(5):438-442. Shen S X,Li Z Q,Zhang C H,et al. Identification of double triplo-3,6 and acquisition of primary triplo-3 and triplo-6 in Chinese Cabbage[J].Acta Horticulturae Sinica,2002,29(5):438-442.
18. 袁国良. 用三倍体做母本实现百合种质渗入育种[D].杭州:浙江大学,2014. Yuan G L. Lily introgression breeding using triploids cultivars as maternal parents[D].Hangzhou:Zhejiang University,2014.
19. 李克虎. 异源三倍体百合(Lilium)育种潜力研究[D].杭州:浙江大学,2011. Li K H. Study on the potential of allotriploid lilies(Lilium) in breeding[D].Hangzhou:Zhejiang University,2011.

[1]	穆赢通, 樊东昌, 吕丽娟, 李晓杰, 路景诗, 张晓明. 毛茛科和芍药科叶绿体基因组密码子特征和系统发育比较[J]. 植物研究, 2022, 42(6): 964-975.
[2]	汪颖, 张玉洁, 焦子惠, 李雪丽, 王凯, 达晓伟, 孙坤, 张辉. 中国‘苦水’玫瑰杂交育性的初步研究[J]. 植物研究, 2022, 42(3): 403-411.
[3]	魏冬霞, 张滕, 郑严仪, 于晓南. 芍药愈伤组织中体细胞胚发育过程的组织细胞学观察[J]. 植物研究, 2018, 38(1): 56-63.
[4]	丁海燕. 植物材料减数分裂染色体标本的快速制作[J]. 植物研究, 2017, 37(5): 797-800.
[5]	杨柳慧, 张建军, 王琪, 朱炜, 张滕, 于晓南. 5个芍药属伊藤杂种的倍性鉴定及核型分析[J]. 植物研究, 2017, 37(4): 535-541.
[6]	吴杰, 赵玉萍, 何祥凤, 王文和, 冷平生, 张克中. OT杂种系百合品种‘Cocossa’小孢子败育细胞学研究[J]. 植物研究, 2017, 37(1): 15-22.
[7]	李云玲, 田保明, 杨妍, 崔明珠, 郝志达, 位芳. 拟南芥同源四倍体减数分裂过程的分子细胞学分析[J]. 植物研究, 2016, 36(3): 354-359.
[8]	魏冬霞1,2,3；高凯4；袁燕波1；汤正娇1；于晓南1,2,3*. 观赏芍药幼胚不定芽离体诱导研究[J]. 植物研究, 2016, 36(2): 190-194.
[9]	曲文静;李青*;刘燕. 芍药组织培养中地下芽污染的克服[J]. 植物研究, 2014, 34(4): 524-528.
[10]	赵连飞;陈凤丽;孙曦晓;杜新琦;赵春建*;姚欢欢;杨磊;汪贵斌;祖元刚. 牡丹种壳中丹皮酚和芍药苷的微波提取及含量测定[J]. 植物研究, 2014, 34(4): 561-566.
[11]	周启超;姚欢欢;陈凤丽;杜新琦;赵春建*;王化;杨磊;汪贵斌;祖元刚. 响应面法优选牡丹果荚中丹皮酚和芍药苷提取工艺的研究[J]. 植物研究, 2014, 34(2): 271-275.
[12]	王吉凤;李青;包欣. 芍药组织培养中污染现象的克服[J]. 植物研究, 2012, 32(1): 84-90.
[13]	闫小丹;张延明;李集临*. 八倍体小偃麦与天蓝偃麦草杂交F1染色体组构型[J]. 植物研究, 2010, 30(2): 197-201.
[14]	张新宇;王赞;赵茂林;高洪文*;刘大林. 两种山羊豆属植物花粉形态与花粉母细胞减数分裂行为观察[J]. 植物研究, 2008, 28(5): 540-546.
[15]	陈　霞;邢璐璐;丁海燕;徐英博;李集临*. GC1代换系与小麦杂交减数分裂行为的研究[J]. 植物研究, 2008, 28(4): 392-395.

三倍体芍药品种花粉母细胞减数分裂行为观察与比较

Meiosis Observation and Comparison of Pollen Mother Cells of Triploid Paeonia lactiflora Cultivars

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价