狭叶金星蕨的快速繁殖

doi:10.7525/j.issn.1673-5102.2015.06.005

植物研究 ›› 2015, Vol. 35 ›› Issue (6): 819-824.doi: 10.7525/j.issn.1673-5102.2015.06.005

狭叶金星蕨的快速繁殖

陈习^1,2,3；何美娜^1,2,3；庄卉卉^1,2,3；郭丽珠^1,2,3；周攀^1,2,3；何玮^1,2,3；郭斌^1,2,3；尉亚辉^1,2,3*

1.西部资源生物与现代生物技术省部共建教育部重点实验室，西安　710069；
2.陕西省生物技术重点实验室，西安　710069；
3.西北大学生命科学学院，西安　710069

出版日期:2015-11-20 发布日期:2016-01-18
基金资助:

Rapid Propagation of Parathelypteris angustifrons(Miq.) Ching

CHEN Xi^1,2,3；HE Mei-Na^1,2,3；ZHUANG Hui-Hui^1,2,3；GUO Li-Zhu^1,2,3；ZHOU Pan^1,2,3；HE Wei^1,2,3；GUO Bin^1,2,3；WEI Ya-Hui^1,2,3*

1.Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education,Xi’an　710069；
2.Shanxi Provincial Key Laboratory of Biotechnology,Xi’an　710069；
3.College of Life Science,Northwest University,Xi’an　710069

Online:2015-11-20 Published:2016-01-18
Supported by:

摘要/Abstract

摘要： The rapid propagation system of Huperzia serrata(Thunb.) Trev. associated fern (Parathelypteris angustifrons(Miq.) Ching) was established by culturing its prothallium. MS+1.0 mg·L^-1 NAA was the most suitable medium for prothallium proliferation. After 60 days of growing in this medium, the fresh weight of prothallium weighed twice heavier compared with one in the control medium (MS without hormone). MS without plant hormone was the optimal medium for inducing sporophyte. The shortest induction time was 22 days and the induction rate was 15%. 6-BA and TDZ showed inhibitory effect on the formation of the sporophyte. The best medium for root induction of sporophyte was MS+1.0 mg·L^-1 IAA. Within 60 days, the medium induced a mean root length of 1.72 cm and 13 roots per explant. Sporophyte grew best in water moss by which the survival rate of transplantation reached 100%.

关键词: 狭叶金星蕨, 原叶体, 快速繁殖

Abstract: The rapid propagation system of Huperzia serrata(Thunb.) Trev. associated fern (Parathelypteris angustifrons(Miq.) Ching) was established by culturing its prothallium. MS+1.0 mg·L^-1 NAA was the most suitable medium for prothallium proliferation. After 60 days of growing in this medium, the fresh weight of prothallium weighed twice heavier compared with one in the control medium (MS without hormone). MS without plant hormone was the optimal medium for inducing sporophyte. The shortest induction time was 22 days and the induction rate was 15%. 6-BA and TDZ showed inhibitory effect on the formation of the sporophyte. The best medium for root induction of sporophyte was MS+1.0 mg·L^-1 IAA. Within 60 days, the medium induced a mean root length of 1.72 cm and 13 roots per explant. Sporophyte grew best in water moss by which the survival rate of transplantation reached 100%.

Key words: Parathelypteris angustifrons, prothallium, rapid propagation

中图分类号:

陈习1,2,3；何美娜1,2,3；庄卉卉1,2,3；郭丽珠1,2,3；周攀1,2,3；何玮1,2,3；郭斌1,2,3；尉亚辉1,2,3*. 狭叶金星蕨的快速繁殖[J]. 植物研究, 2015, 35(6): 819-824.

CHEN Xi1,2,3；HE Mei-Na1,2,3；ZHUANG Hui-Hui1,2,3；GUO Li-Zhu1,2,3；ZHOU Pan1,2,3；HE Wei1,2,3；GUO Bin1,2,3；WEI Ya-Hui1,2,3*. Rapid Propagation of Parathelypteris angustifrons(Miq.) Ching[J]. Bulletin of Botanical Research, 2015, 35(6): 819-824.

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狭叶金星蕨的快速繁殖

Rapid Propagation of Parathelypteris angustifrons(Miq.) Ching

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