关键词: 黄檗, 滤光膜, 植株再生, 抗氧化酶, 可溶性蛋白

Abstract: The stems of sterile plantlets of Phellodendron amurense were cultured on MS medium supplemented with 1.5 mg·L^-1 6-BA and 0.5 mg·L^-1 NAA, the effects of different film filters on shoot regeneration from stem explants of P.amurense and some physiological and biochemical indexes changes during shoot regeneration were investigated. The results showed that there was obvious promotion of blue films to shoot regeneration and the highest percentage of shoot regeneration(75.4%) with the maximum number of shoots per callus(14.7) were observed under blue films, followed by fluorescent light and yellow films. However, red and green films were ineffective for shoot regeneration. In this study, it was found that blue films and fluorescent light induced lower chlorophyll a/b ratio and higher level of chlorophyll content than other treatment. Antioxidant enzymes activity and soluble protein were the highest under blue films, followed by fluorescent light, the lowest under green ones.

Key words: Phellodendron amurense, color film, shoot regeneration, antioxidant enzyme, soluble protein