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植物研究 ›› 2009, Vol. 29 ›› Issue (5): 603-606.doi: 10.7525/j.issn.1673-5102.2009.05.017

• 论文 • 上一篇    下一篇

欧美杨108号转蜘蛛杀虫肽与Bt毒蛋白嵌合基因的研究

遇文婧;邹传山;王志英*;张国财   

  1. (东北林业大学林学院,哈尔滨150040)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-09-20 发布日期:2009-09-20
  • 通讯作者: 王志英
  • 基金资助:
     

Populus nigra×Populus deltoids “108” Transformed the Chimeric Genes of Spider Insecticidal and Bt

YU Wen-Jing;ZOU Chuan-Shan;WANG Zhi-Ying*;ZHANG Guo-Cai   

  1. (College of Forestry,Northeast Forestry University,Harbin150040)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
  • Contact: WANG Zhi-Ying
  • Supported by:
     

摘要: 以欧美杨108号为外植体材料,利用农杆菌介导法成功地将抗虫基因(蜘蛛杀虫肽+Bt毒蛋白的嵌合基因)导入受体中。通过PCR检测,凝胶电泳结果显示8个卡那霉素抗性芽中,6个存在目的基因;经PCR-Southern印迹杂交检测,阳性率为50%,进一步表明目的基因成功整合到欧美杨108号基因组内。

关键词: 欧美杨108号, 蜘蛛杀虫肽+Bt毒蛋白基因, PCR-Southern印迹杂交

Abstract: Using the agrobacterium-mediated approach, the anti-insect gene (the chimeric genes of spider insecticidal and Bt) was successfully transformed into the acceptor of Populus nigra×Populus deltoids “108” as explant. Through PCR analysis, the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes; Through PCRSouthern blots analysis, the result showed that the positive rate was 50%, and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids “108” plants successfully.

Key words: Populus nigra×, Populus deltoids &ldquo, 108&rdquo, the chimeric genes of spider insecticidal and Bt, PCR-Southern blots analysis

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