硒处理对斜茎黄芪生长生理和初生代谢的影响

doi:10.7525/j.issn.1673-5102.2022.06.016

植物研究 ›› 2022, Vol. 42 ›› Issue (6): 1062-1069.doi: 10.7525/j.issn.1673-5102.2022.06.016

硒处理对斜茎黄芪生长生理和初生代谢的影响

姜婉婷¹^,², 杨楠¹^,², 陈晨¹^,², 郭晓瑞¹^,², 唐中华¹^,², 王洪政¹^,²()

^1.东北林业大学化学化工与资源利用学院，哈尔滨 150040
^2.东北林业大学森林植物生态学教育部重点实验室，哈尔滨 150040

收稿日期:2022-01-25 出版日期:2022-11-20 发布日期:2022-11-22
通讯作者: 王洪政 E-mail:hzwang@nefu.edu.cn
作者简介:姜婉婷（1995—），女，硕士研究生，主要从事药用植物环境胁迫及次生代谢研究。
基金资助:
科技部基础性工作专项(2019FY100505)

Effects of Selenium Treatment on Physiology and Primary Metabolism of Astragalus adsurgens

Wanting JIANG¹^,², Nan YANG¹^,², Chen CHEN¹^,², Xiaorui GUO¹^,², Zhonghua TANG¹^,², Hongzheng WANG¹^,²()

^1.College of Chemistry，Chemical Engineering and Resource Utilization，Northeast Forestry University，Harbin 150040
^2.The Key Laboratory of Forest Plant Ecology Ministry of Education，Northeast Forestry University，Harbin 150040

Received:2022-01-25 Online:2022-11-20 Published:2022-11-22
Contact: Hongzheng WANG E-mail:hzwang@nefu.edu.cn
About author:JIANG Wanting（1995—），female，graduate student，mainly engaged in the study of environmental stress and secondary metabolism the medicine plants.
Supported by:
Fundamental Work Special Project of the Ministry of Science and Technology(2019FY100505)

摘要/Abstract

摘要：

以一种具有较高Se富集能力的植物——斜茎黄芪（Astragalus adsurgens）为试验材料，在水培条件下研究不同浓度Se处理对其生长发育、光合参数和初生代谢产物积累的影响。结果表明：5 μmol·L^-1 Se处理显著促进了斜茎黄芪根系和地上部的伸长生长；100 μmol·L^-1 Se处理则具有相反效果。光合色素含量和叶片叶绿素荧光参数研究结果与生长指标相一致，低浓度Se对斜茎黄芪叶片的光合作用具有促进作用，高浓度Se则对其具有明显的抑制作用。随着处理浓度的升高，斜茎黄芪根系和地上部Se含量逐渐增加，但Se的转运系数明显降低，100 μmol·L^-1处理下Se转运系数比5 μmol·L^-1处理降低了83.5%。通过对初生代谢产物进行检测和分析，发现低浓度Se处理主要上调了斜茎黄芪中与氨基酸代谢相关途径的代谢水平，而高浓度Se处理主要上调了与次生代谢物质合成关系密切的初生代谢产物的代谢水平。我们的研究结果表明，低浓度Se处理提高了斜茎黄芪光合作用水平以及与生长相关的初生代谢水平，从而促进了植株生长；高浓度Se处理下斜茎黄芪则通过降低Se向地上部的转运水平并将更多的初生代谢产物用于次生代谢产物的合成，从而提高了植株对Se胁迫的耐性。

关键词: 斜茎黄芪, 硒处理, 初生代谢产物, 代谢通路

Abstract:

In order to clarify the effects of different concentration Se treatments on the growth， development， photosynthesis and the primary metabolism of plant， Astragalus adsurgens， with high Se accumulation capacity under hydroponic condition was used as material. The results showed that different concentrations of Se had different effects on the growth and development of A. adsurgens. Low concentration Se treatment（5 μmol·L^-1） significantly promoted the growth of A. adsurgens， whereas high concentration Se treatment（100 μmol·L^-1） had the opposite effect. The content of Se in roots and shoots was positively correlated with the treatment concentration， and the transport coefficient of Se in A. adsurgens decreased gradually with the increase of selenium concentration. The results of photosynthetic pigment content and leaf chlorophyll fluorescence parameters showed that low-concentrations of Se promoted photosynthesis of A. adsurgens， while high-concentrations of Se significantly inhibited it， the Se transport coefficient under 100 μmol·L^-1 treatment decreased by 83.5% compared with 5 μmol·L^-1 treatment. After detection and analysis of primary metabolites， it was found that low concentrations of Se up-regulated the metabolic pathways related to amino acid metabolism in plant roots and shoots， while high concentrations of Se mainly up-regulated the metabolic levels of primary metabolites that were closely related to the synthesis of secondary metabolites. Our results showed that treatment with low concentration of Se increased the photosynthesis level of A. adsurgens and the level of primary metabolism related to growth， and promoted plant growth； treatment with high concentration Se increased the level of A. adsurgens by reducing the transport of Se to the ground and more primary metabolites for the synthesis of secondary metabolites were used， and the tolerance of plants to Se stress was improved.

Key words: Astragalus adsurgens, selenium treatment, primary metabolites, metabolic pathways

中图分类号:

Q949.751.9

姜婉婷, 杨楠, 陈晨, 郭晓瑞, 唐中华, 王洪政. 硒处理对斜茎黄芪生长生理和初生代谢的影响[J]. 植物研究, 2022, 42(6): 1062-1069.

Wanting JIANG, Nan YANG, Chen CHEN, Xiaorui GUO, Zhonghua TANG, Hongzheng WANG. Effects of Selenium Treatment on Physiology and Primary Metabolism of Astragalus adsurgens[J]. Bulletin of Botanical Research, 2022, 42(6): 1062-1069.

图/表 8

图1

图2

图3

图4

表1

图5

表2

图6

参考文献 18

1	NAWAZ F， ASHRAF M Y， AHMAD R，et al.Supplemental selenium improves wheat grain yield and quality through alterations in biochemical processes under normal and water deficit conditions［J］.Food Chemistry，2015，175：350-357.
2	孔凡丽，张恩萍，曹庆军，等.硒的生理功能及在主要作物中的吸收富集［J］.东北农业科学，2020，45（6）：115-118.
	KONG F L， ZHANG E P， CAO Q J，et al.Physiological function and absorption enrichment of selenium in staple crops［J］.Journal of Northeast Agricultural Sciences，2020，45（6）：115-118.
3	LEHOTAI N， LYUBENOVA L， SCHRÖDER P，et al.Nitro-oxidative stress contributes to selenite toxicity in pea（Pisum sativum L）［J］.Plant and Soil，2016，400（1/2）：107-122.
4	TERRY N， ZAYED A M， DE SOUZA M P，et al.Selenium in higher plants［J］.Annual Review of Plant Physiology & Plant Molecular Biology，2000，51：401-432.
5	李登超，朱祝军，徐志豪，等.硒对小白菜生长和养分吸收的影响［J］.植物营养与肥料学报，2003，9（3）：353-358.
	LI D C， ZHU Z J， XU Z H，et al.Effects of selenium on the growth and nutrient absorption of pakchoi［J］.Plant Nutrition and Fertilizing Science，2003，9（3）：353-358.
6	CABRAL GOUVEIA G C， GALINDO F S， DANTAS BERETA LANZA M G，et al.Selenium toxicity stress-induced phenotypical，biochemical and physiological responses in rice plants：characterization of symptoms and plant metabolic adjustment［J］.Ecotoxicology and Environmental Safety，2020，202：110916.
7	孙丽萍，郑尚珍，沈序维.直立黄芪中黄酮类似物的研究［J］.西北师范大学学报（自然科学版），1994，30（3）：52-54.
	SUN L P， ZHENG S Z， SHEN X W.Studies on flavonoids fo Astragalus adsurgens Pall.［J］.Jouranl of Northwest Normal University （Natural Science Edition），1994，30（3）：52-54.
8	Montana Plant Life. Astragalus adsurgens Pallas［DB/OL］.［2022-04-19］..
9	程贵文，龚洪恩，颜送宝，等.油茶叶绿素提取方法的比较研究［J］.湖北林业科技，2017，46（6）：11-13，58.
	CHENG G W， GONG H E， YAN S B，et al.Comparison of chlorophyll extraction methods in Camellia oleifera ［J］.Hubei Forestry Science and Technology，2017，46（6）：11-13，58.
10	CEN H Y， WENG H Y， YAO J N，et al.Chlorophyll fluorescence imaging uncovers photosynthetic fingerprint of Citrus huanglongbing［J］.Frontiers in Plant Science，2017，8：1509.
11	马月花，郭晓瑞，杨楠，等.黄芪幼苗对镉胁迫的生理响应机制［J］.植物研究，2019，39（4）：497-504.
	MA Y H， GUO X R， YANG N，et al.Physiological mechanisms in Astragalus membranaceus seedlings responding to cadmium stress［J］.Bulletin of Botanical Research，2019，39（4）：497-504.
12	刘洋.应用代谢组学对比研究两种黄芪响应UV-B和干旱胁迫的代谢基础［D］.哈尔滨：东北林业大学，2018.
	LIU Y.Comparative metabolomics study on the metabolic basis of two varieties of Astragalus in response to UV-B and drought stress［D］.Harbin：Northeast Forestry University，2018.
13	郭坤元，何银生，林先明，等.亚硒酸盐对野葛幼苗形态及生理指标的影响［J］.中药材，2020，43（3）：532-535.
	GUO K Y， HE Y S， LIN X M，et al.Effects of selenite on morphology and physiological indexes of kudzu seedlings［J］.Journal of Chinese Medicinal Materials，2020，43（3）：532-535.
14	SHENG M， TANG M， CHEN H，et al.Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress［J］.Mycorrhiza，2008，18（6/7）：287-296.
15	LIU M H， YI L T， YU S Q，et al.Chlorophyll fluorescence characteristics and the growth response of Elaeocarpus glabripetalus to simulated acid rain［J］.Photosynthetica，2015，53（1）：23-28.
16	WU Y W， LI Q， JIN R，et al.Effect of low-nitrogen stress on photosynthesis and chlorophyll fluorescence characteristics of maize cultivars with different low-nitrogen tolerances［J］.Journal of Integrative Agriculture，2019，18（6）：1246-1256.
17	ÇAKIR Ö， TURGUT-KARA N，ARI Ş，et al. De novo transcriptome assembly and comparative analysis elucidate complicated mechanism regulating Astragalus chrysochlorus response to selenium stimuli［J］.PLoS One，2015，10（10）：e0135677.
18	王永勤，曹家树，李建华，等.施硒对大蒜产量和含硒量的影响［J］.园艺学报，2001，28（5）：425-429.
	WANG Y Q， CAO J S， LI J H，et al.Effect of selenium application on the yield of garlic and its selenium content［J］.Acta Horticulturae Sinica，2001，28（5）：425-429.

部位 Part	处理 Treatment （μmol·L^-1）	差异代谢物总数 Total differential metabolites	上调化合物 Up-regulated compounds	下调化合物 Down-regulated compounds
地上部 Shoot	5	35	22	9
地上部 Shoot	100	31	24	7
根系 Root	5	15	11	4
根系 Root	100	29	21	8

[1]	吴艳, 李赛, 吴可心, 穆立蔷. 基于GC-MS的野生和‘鲁赫’刺蔷薇叶片代谢差异研究[J]. 植物研究, 2022, 42(6): 1070-1078.
[2]	叶兴状, 刘丹, 罗佳佳, 范辉华, 张国防, 刘宝, 陈世品. 濒危珍稀植物半枫荷的转录组分析[J]. 植物研究, 2019, 39(2): 276-286.

硒处理对斜茎黄芪生长生理和初生代谢的影响

Effects of Selenium Treatment on Physiology and Primary Metabolism of Astragalus adsurgens

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 18

相关文章 2

编辑推荐

Metrics

本文评价