北疆地区同域分布的沙拐枣属植物是否存在杂交初探

doi:10.7525/j.issn.1673-5102.2016.05.022

植物研究 ›› 2016, Vol. 36 ›› Issue (5): 790-794.doi: 10.7525/j.issn.1673-5102.2016.05.022

北疆地区同域分布的沙拐枣属植物是否存在杂交初探

康晓珊^1,2, 索菲娅³, 段士民^1,2, 潘伯荣^1,2, 张永智², 田聪⁴

1. 中国科学院新疆生态与地理研究所, 中国科学院干旱区生物地理与生物资源重点实验室, 乌鲁木齐 830011;
2. 中国科学院新疆生态与地理研究所吐鲁番沙漠植物园, 新疆吐鲁番 830083;
3. 新疆大学生命科学与技术学院, 乌鲁木齐 830046;
4. 新疆畜牧科学院草业研究所, 乌鲁木齐 830000

收稿日期:2016-04-26 出版日期:2016-09-15 发布日期:2016-09-27
作者简介:康晓珊(1982-),女,博士,助理研究员,主要从事繁殖生物学研究。
基金资助:
国家自然科学青年基金资助项目（31200237）；国家自然科学基金面上项目（41271069）

Preliminary Studies on Hybridization among Sympatric Distributed Calligonum Species in Northern Xinjiang

KANG Xiao-Shan^1,2, Sophia³, DUAN Shi-Min^1,2, PAN Bo-Rong^1,2, ZHANG Yong-Zhi², TIAN Cong⁴

1. Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Key Laboratory of Biogeography and Bioresource in Arid Land, Urumqi 830011;
2. Turpan Eremophytes Botanical Garden, Chinese Academy of Sciences, Turpan 838008;
3. College of Life Science and Technology, Xinjiang University, Urumqi 830046;
4. Grassland Institute, Xinjiang Academy of Animal Science, Urumqi 830000

Received:2016-04-26 Online:2016-09-15 Published:2016-09-27
Supported by:
This work was supported by National Natural Science Foundation of China NSFC(No.31200237 and 41271069)

摘要/Abstract

摘要： 杂交问题一直是沙拐枣属植物分类的核心问题。本文通过对自然生境和植物园的开花物候观测，人工授粉杂交实验以及花粉管荧光显色实验来探讨同域分布的沙拐枣属植物是否存在杂交，结果显示：同域分布种间均存在花期重叠；人工杂交授粉坐果率极低，且无法正常萌发，花粉管荧光显微观察实验表明杂交花粉能在柱头上萌发，但是花粉管不能伸长到子房。综上实验结果表明，种间配子亲和性很低，既存在合子前隔离，又存在合子后生殖隔离，认为该属不存在杂交。开展此项研究对于了解同域分布沙拐枣属植物间的隔离分化具有重要意义；为该属不存在杂交提供有力证据，也为该属的分类研究开拓了新的思路。

关键词: 沙拐枣属(Calligonum L.), 开花物候, 杂交, 花粉管荧光显微

Abstract: Hybridization is one key issue in taxonomy of the genus Calligonum. To certificate whether or not hybridization are existing among sympatric distributed Calligonum species, observations of flowering phenology both in natural habitats and botanical garden, artificial pollination hybridization experiments and fluorescence microscope observation on cross-pollination of pollen tubes were carried on. Florescence among the sympatric species was overlapped. Fruit rate by artificial pollination was very low, and could not germinate. Pollen tube fluorescent experiments showed that hybrid pollen could germinate on the stigma, but the pollen tube could not stretch to the ovary. Above all, affinity of interspecific gametes was low, reproductive isolation was existing, and hybridization was absent. This study has important implications for understanding the isolation and differentiation of sympatric Calligonum species.

Key words: Calligonum L., flowering phenology, hybridization, fluorescence microscopy

中图分类号:

S793.1

康晓珊, 索菲娅, 段士民, 潘伯荣, 张永智, 田聪. 北疆地区同域分布的沙拐枣属植物是否存在杂交初探[J]. 植物研究, 2016, 36(5): 790-794.

KANG Xiao-Shan, Sophia, DUAN Shi-Min, PAN Bo-Rong, ZHANG Yong-Zhi, TIAN Cong. Preliminary Studies on Hybridization among Sympatric Distributed Calligonum Species in Northern Xinjiang[J]. Bulletin of Botanical Research, 2016, 36(5): 790-794.

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北疆地区同域分布的沙拐枣属植物是否存在杂交初探

Preliminary Studies on Hybridization among Sympatric Distributed Calligonum Species in Northern Xinjiang

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