不同倍性桤木属植物遗传差异的AFLP分析

doi:10.7525/j.issn.1673-5102.2014.06.015

摘要/Abstract

摘要： 采用AFLP技术对桤木属4种不同倍性的116份材料指纹图谱进行了分析,5对引物组合共得到301个位点，282个为多态位点，多态位点百分率为93.7%。4个种群多态位点百分率为35.2%～72.4%，平均多态位点百分率为54.6%。总的Nei’s基因多样性为0.3043，总Shannon多态信息指数为0.4578。种群间及种群内部均具有较丰富的遗传多样性，种群间的遗传差异大于种群内部的遗传差异。NJ聚类分析与主坐标分析结果一致，所有的供试材料明显的分为3个组，地理分布距离较近的种群优先聚在一起。

关键词: 桤木属, AFLP分析, 遗传差异, NJ聚类分析, 主坐标分析

Abstract: We studied the genetic difference between species in Alnus with different ploidies by using AFLP. Five AFLP primers produced 301 bands, of which 282 were polymorphic, accounting for 93.7%. The percentage of polymorphic bands of four population was 35.2% 72.4%, respectively, with an average percentage of 54.6%. The total population of genetic diversity Nei’s was 0.3043, and total Shannon polymorphism information was 0.4578. Among and within populations all had abundant genetic diversity, and the genetic difference interspecific was higher than that of intraspecific. By NJ cluster and PCOA, the closely related populations are geographically restricted and occur in proximity to each other.

Key words: Alnus, karyotype, AFLP, genetic diversity, NJ cluster, PCOA

中图分类号:

饶龙兵1；杨汉波1,2；郭洪英3；段红平2；陈益泰1. 不同倍性桤木属植物遗传差异的AFLP分析[J]. 植物研究, 2014, 34(6): 803-809.

RAO Long-Bing1；YANG Han-Bo1,2；GUO Hong-Ying3；DUAN Hong-Ping2；CHEN Yi-Tai1. AFLP Analysis of Genetic Differences among Alnus with Different Ploidies[J]. Bulletin of Botanical Research, 2014, 34(6): 803-809.

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