牡丹种壳中丹皮酚和芍药苷的微波提取及含量测定

doi:10.7525/j.issn.1673-5102.2014.04.022

植物研究 ›› 2014, Vol. 34 ›› Issue (4): 561-566.doi: 10.7525/j.issn.1673-5102.2014.04.022

牡丹种壳中丹皮酚和芍药苷的微波提取及含量测定

赵连飞¹；陈凤丽¹；孙曦晓¹；杜新琦¹；赵春建^1*；姚欢欢¹；杨磊¹；汪贵斌²；祖元刚¹

1.东北林业大学森林植物生态学教育部重点实验室，哈尔滨　150040；2.南京林业大学森林资源与环境学院，南京　210037

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-07-20 发布日期:2014-07-20
通讯作者: 赵春建
基金资助:

Microwave-assisted Extraction of Paeoniflorin and Paeonol from Tree Peony Seed Coat and Content Determination

ZHAO Lian-Fei;CHEN Feng-Li;SUN Xi-Xiao;DU Xin-Qi;ZHAO Chun-Jian*;YAO Huan-Huan;YANG Lei;WANG Gui-Bin;ZU Yuan-Gang

1.Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin　150040；2.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing　210037

Received:1900-01-01 Revised:1900-01-01 Online:2014-07-20 Published:2014-07-20
Contact: ZHAO Chun-Jian
Supported by:

摘要/Abstract

摘要： 以牡丹种壳为原料，采用微波辅助提取方法，以芍药苷和丹皮酚得率为指标，利用单因素实验对于影响牡丹种壳中芍药苷和丹皮酚得率的因素进行优化。得到的最佳提取工艺参数为：乙醇提取分数70%，液料比10∶1、微波功率230 W、微波时间10 min，浸泡时间1 h,提取次数1次，牡丹种壳芍药苷和丹皮酚的得率分别为2.88和0.52 mg·g^-1。同时，对于选取的微波辅助提取方法的稳定性，回收率和重现性进行验证，结果显示微波辅助提取方法是一个稳定的、可靠的提取方法。

关键词: 牡丹种壳, 芍药苷, 丹皮酚, 微波提取

Abstract: In this study, single factor experiment was used to optimize the main factors of microwave-assisted extraction for paeoniflorin and paeonol from tree peony seed coats. The optimum extraction conditions were as follows: ethanol extract fraction was 70%, liquid-solid ratio was 10∶1, microwave power was 230 W, microwave time was 10 min, soaking time was 1 h and extraction times was one. Under these conditions, the yields of paeoniflorin and paeonol were 2.88 and 0.52 mg·g^-1, respectively. Meanwhile, the proposed method was validated in stability, repeatability and recovery experiments and the proposed method was a stable and efficiency method.

Key words: peony seed coats, Paeoniflorin, Paeonol, microwave-assisted extraction

中图分类号:

S567.1+5

赵连飞;陈凤丽;孙曦晓;杜新琦;赵春建*;姚欢欢;杨磊;汪贵斌;祖元刚. 牡丹种壳中丹皮酚和芍药苷的微波提取及含量测定[J]. 植物研究, 2014, 34(4): 561-566.

ZHAO Lian-Fei;CHEN Feng-Li;SUN Xi-Xiao;DU Xin-Qi;ZHAO Chun-Jian*;YAO Huan-Huan;YANG Lei;WANG Gui-Bin;ZU Yuan-Gang. Microwave-assisted Extraction of Paeoniflorin and Paeonol from Tree Peony Seed Coat and Content Determination[J]. Bulletin of Botanical Research, 2014, 34(4): 561-566.

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牡丹种壳中丹皮酚和芍药苷的微波提取及含量测定

Microwave-assisted Extraction of Paeoniflorin and Paeonol from Tree Peony Seed Coat and Content Determination

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