拟南芥MT-II过量表达提高抗旱性

doi:10.7525/j.issn.1673-5102.2005.04.008

植物研究 ›› 2005, Vol. 25 ›› Issue (4): 415-418.doi: 10.7525/j.issn.1673-5102.2005.04.008

拟南芥MT-II过量表达提高抗旱性

唐中华, 郭晓瑞, 张洋洋, 安志刚, 于景华, 祖元刚

东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040

收稿日期:2005-07-28 出版日期:2005-12-15 发布日期:2016-06-14
通讯作者: 祖元刚 E-mail:zygorl@public.hr.h.lcn
作者简介:Tang Zhonghua(1977-), Male, Assistant, Major in plant secondary metabolism and molecular biology.

Expression of Metallothionein-II in Arabidopsis thaliana improve desiccation tolerance

TANG Zhong-Hua, GUO Xiao-Rui, ZHANG Yang-Yang, AN Zhi-Gang, YU Jing-Hua, ZU Yuan-Gang

Key Laboratory of Forest Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040

Received:2005-07-28 Online:2005-12-15 Published:2016-06-14
Supported by:
Supported by the State Key Basic Research Project (G19990160) and the Key Project of Chinese Ministry of Education (104191)

摘要/Abstract

摘要： 富含巯基的植物II型金属硫蛋白(MT)对植物抵抗重金属胁迫具有重要作用,其中一个可能机制是金属硫蛋白可能猝灭重金属引起的氧化胁迫。利用转MT-II基因和野生型拟南芥(Arabidopsis thaliana)植株来对比研究MT在胁迫过程中通过清除氧自由基,特别是H₂O₂而对植物抗旱性的影响。研究表明,转基因型拟南芥能有效维持体内氧化-还原势,减少MDA的产生,从而缓解干旱胁迫引起的伤害,提高抗旱性。

关键词: 植物II型金属硫蛋白, 干旱抗性, 氧化胁迫

Abstract: Metallothionein-II(MT-II) has been identified to play an important role in the exposure of plant to heavy metal like Cd and Cu.One of the mechanisms of MT's proposed to protect plants during this process is that it can quench the reactive oxygen species(ROS) activated by heavy-metal exposure.Here we demonstrated the effect of expression of MT-II in Arabidopsis thaliana on oxidative stress and damage extent during drought stress. The RT-PCR results suggested the implication of MT-II against drought stress in the transgenic Arabidopsis.Measurements of H₂O₂ and malondialdehyde (MDA) indicated that over-expression of MT-II in A.thaliana improved desiccation tolerance.

Key words: MT-II, desiccation tolerance, scavenging ROS

唐中华, 郭晓瑞, 张洋洋, 安志刚, 于景华, 祖元刚. 拟南芥MT-II过量表达提高抗旱性[J]. 植物研究, 2005, 25(4): 415-418.

TANG Zhong-Hua, GUO Xiao-Rui, ZHANG Yang-Yang, AN Zhi-Gang, YU Jing-Hua, ZU Yuan-Gang. Expression of Metallothionein-II in Arabidopsis thaliana improve desiccation tolerance[J]. Bulletin of Botanical Research, 2005, 25(4): 415-418.

拟南芥MT-II过量表达提高抗旱性

Expression of Metallothionein-II in Arabidopsis thaliana improve desiccation tolerance

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