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植物研究 ›› 2004, Vol. 24 ›› Issue (4): 477-481.

• 论文 • 上一篇    下一篇

中国东北15种藓类植物RAPD分析及其分类学意义

侯义龙1,2, 曹同3   

  1. 1. 大连大学生命科学工作室, 大连 116622;
    2. 大连大学生物工程学院, 大连 116622;
    3. 上海师范大学生命与环境科学学院, 上海 200234
  • 收稿日期:2004-01-07 出版日期:2004-12-15 发布日期:2016-06-14
  • 作者简介:侯义龙(1963-), 男, 博士, 教授, 从事植物生物技术研究工作, 中国科学院沈阳应用生态研究所博士后。
  • 基金资助:
    国家自然科学基金(项目编号 30170076)

RAPD analysis of 15 mosses species from Northeast China and its taxonomic implication

HOU Yi-Long1,2, CAO Tong3   

  1. 1. Laboratory of Life Science, Dalian University, Dalian 116622;
    2. Bioengineering College, Dalian University, Dalian 116622;
    3. College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234
  • Received:2004-01-07 Online:2004-12-15 Published:2016-06-14

摘要: 本研究选用15 种东北藓类植物, 其中塔藓、赤茎藓、垂枝藓、拟垂枝藓、羽平藓、木藓、万年藓等为北半球广泛分布的大型藓类, 而褶叶藓、粗枝藓、球蒴金发藓等则是东亚特有种。在野外调查和采集的基础上, 进行RAPD 分析, 旨在从DNA 水平上对它们的亲缘关系和分类问题加以研究, 为它们的分类问题和亲缘关系提供分子证据。结果:从分子水平上进一步验证了15 种东北藓类植物形态分类的正确性。不同种类分别聚在金发藓目(Polytrichinales), 变齿藓目(Isobryales)和灰藓目(Hypnobryales)中, 与现采用分类系统一致。东北特产属褶叶藓属(Okamuraea)和粗枝藓属(Gollania)与垂枝藓科(Hyitidiaceae)植物关系较近, 而赤茎藓(Pleurozium)与绢藓(Entodon)有一定亲缘关系。因此, 将赤茎藓置于塔藓科(Hylocomiaceae)是合适的。

关键词: 中国东北, 藓类植物, RAPD, 分类学, 亲缘关系

Abstract: The random amplified polymorphic DNA(RAPD)was used to analyse the phylogenetic relationship among fifteen mosses species from Northeast China in which Hylocomium Splendens, Pleurozium schreberi, Rhytidium rugosum, Rhytidiadelphus squarosus, Neckera pennata, Thamnobryum alopecurum and Climacium dendroides distribute extensively in northern semiglobe, and Okamuraea brachydictyon, Gollania varians and Polytrichum sphaerothcium are representative species endemic to East Asia.The results showed that the RAPD results support the classification of these mosses based on morphology.All the 15mosses species were clustered in Polytrichinales, Isobryales and Hypnobryales.Okamuraea and Gollania have a closer phylogenetic relationship with Hyitidiaceae.Pleurozium has a closer phylogenetic relationship with Entodon, so it is suitable for Pleurozium to be placed in Hylocomiaceae.

Key words: Northeast China, mosses, RAPD analysis, taxonomy, phylogenetic relationship