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植物研究 ›› 2003, Vol. 23 ›› Issue (3): 317-322.

• 论文 • 上一篇    下一篇

用ISSR标记研究高卢蜜环菌系统发生学的尝试

孙立夫1, 杨国亭1,2, 秦国夫3, 宋玉双3, 宋瑞清1   

  1. 1. 东北林业大学, 哈尔滨 150040;
    2. 黑龙江省林业厅, 哈尔滨 150000;
    3. 国家林业局森林病虫害防治总站, 沈阳 110034
  • 收稿日期:2003-01-03 出版日期:2003-09-15 发布日期:2016-06-14
  • 作者简介:孙立夫(1969-),男,在读博士,主要从事蜜环菌生物种方面的研究工作。
  • 基金资助:
    黑龙江省自然科学基金项目的资助(220-413504)和国家自然科学基金项目的资助(30070015)

AN ATTEMPET ABOUT STUDIES ON THE PHYLOGENETICS OF ARMILLARIA GALLICA WITH ISSR MARKER

SUN Li-Fu1, YANG Guo-Ting1,2, QIN Guo-Fu3, SONG Yu-Shuang3, SONG Rui-Qing1   

  1. 1. Northeast Forestry University, Harbin 150040;
    2. Forestry Bureau of Heilongjiang Province, Harbin 150000;
    3. Forest Plant Diseases and Insect Pests Control General Station, National Forestry General Bureau of China, Shenyang 110034
  • Received:2003-01-03 Online:2003-09-15 Published:2016-06-14

摘要: 用ISSR(Inter-Simple Sequence Repeat)标记对黑龙江省牡丹江地区高卢蜜环菌的系统发生学进行了研究,用6个引物对35个菌株的DNA模板进行扩增。结果表明该地区的35个菌株分属3个不同的发育系,并且3个发育系在地理分布上是交错在一起的。同时表明ISSR标记是研究蜜环菌系统发育关系的理想的分子标记手段。

关键词: 高卢蜜环菌, 系统发生学, ISSR

Abstract: The phylogenetics of Armillaria gallica in Mudanjiang area of Heilongjiang Province was studied with ISSR marker. 35 isolates were amplified with 6 primers. The results showed that these 35 isolates belonged to 3 different clades in the area, and these clades interlaced each other in some aeras. It also showed that ISSR marker was an ideal molecular marker in studying the phylogenetics of Armillaria.

Key words: Armillaria gallica, phylogenetics, ISSR