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植物研究 ›› 2003, Vol. 23 ›› Issue (1): 66-71.

• 论文 • 上一篇    下一篇

云南松外生菌根真菌分离培养研究

于富强, 纪大干, 刘培贵   

  1. 中国科学院昆明植物研究所, 昆明 650204
  • 收稿日期:2002-08-16 出版日期:2003-03-15 发布日期:2016-06-14
  • 作者简介:于富强(1976-),男,山东人,硕士,主要从事真菌分类、菌根学及食用菌引种驯化研究。
  • 基金资助:
    国家自然科学基金(No30070004);云南省自然科学基金(No2000C0068M)

STUDIES ON PURE CULTURE OF THE ECTOMYCORRHIZAL FUNGI ASSOCIATED WITH PINUS YUNNANENSIS

YU Fu-Qiang, JI Da-Gan, LIU Pei-Gui   

  1. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204
  • Received:2002-08-16 Online:2003-03-15 Published:2016-06-14

摘要: 就滇中一带云南松林进行了多次调查,共采集、鉴定标本130余号;从中选取13科, 19属, 33种真菌进行了菌种分离、培养的研究。选用并修改4种培养基进行了外生菌根真菌分离、培养的比较,结果共有11科, 17属, 25种真菌分离获得成功,成功率高达75.8%, 4种培养基的分离成功率依次为36.4%、63.6%、33.3%和57.6%。其中,修改PDA培养基和松针、玉米培养基较适宜于云南松外生菌根真菌的分离、纯化和培养。

关键词: 云南松, 外生菌根真菌, 纯培养

Abstract: Several surveys were carried out under Pinus yunnanensis Franch forest in Central Yunnan; the total collections are over 130. 33 species, belonging to 19 genera, 13 families were selected to supply a diversity of pure cultures. 4 types of media were selected for the pure culture study of ectomycorrhizal fungi. As a result, pure cultures of 25 species, 17 genera, and 11 families were obtained. The total isolation success rate reached 75.8%; the success rate of 4 media (No.1, No2, No.3, No.4) was 36.4%, 63.6%, 33.3% and 57.6% respectively. Modified PDA medium (No.2) and pine needles medium (No.4) are more suitable for the isolation, purification and maintenance of ectomycorrhizal fungi associated with P. yunnanensis.

Key words: Pinus yunnanensis Franch, ectomycorrhizal fungi, pure culture