关键词: 大豆, 水稻, 外源DNA导入, 浸种及幼苗期浇灌法, 花粉管通道法, 糙米粗蛋白含量, 氨基酸含量

Abstract: DNA was extracted and purified from soybean seeds. The exogenous DNA from soybean was introduced into rice seeds by soaking seeds, moreover irrigating seedlings, and through the pollen-tube pathway after self-pollination. The contents of crude protein and amino acid in the offspring's seeds were analyzed. The results indicated: (1).The average contents of crude protein in three groups of the offspring's seeds induced by exogenous DNA from soybean were 16.42%、16.80% and 19.87%(Table 1), increasing distinctly over their contrasts.(2). All of the contents of amino acid (except try) in some offspring's seeds induced by exogenous DNA from soybean were 17.20%、16.86% and 16.09%(Table 2), greatly increasing over the contrasts. The lysine contents in the three offspring's seeds were respectively 0.60%、0.60% and 0.57% (Table 2).

Key words: Rice, Soybean, Exogenous DNA, crude prote in content, amino acid content