大蒜根尖细胞有丝分裂同步化诱导与中期染色体分离

植物研究 ›› 1999, Vol. 19 ›› Issue (3): 302-307.

大蒜根尖细胞有丝分裂同步化诱导与中期染色体分离

彭永康¹, 赵建², 陈瑞阳²

1. 天津师范大学生物系, 天津 300074;
2. 南开大学生物系, 天津 300071

收稿日期:1998-07-06 出版日期:1999-09-15 发布日期:2016-06-14
基金资助:
国家教委跨世纪优秀人才基金资助项目。

INDUCTION OF SYNCHRONY AND ISOLATION OF METAPHASE CHROMSOMES FROM MITOTIC ROOT TIP CELLS IN ALLIUM SATIVUM

Peng Yong-kang¹, Zhao Jian², Chen Rui-yang²

1. Department of Biology, Tianjin Normal University, Tianjin 300074;
2. Department of Biology, Nankai University, Tianjin 300071

Received:1998-07-06 Online:1999-09-15 Published:2016-06-14

摘要/Abstract

摘要： 本研究以大蒜(Allium sativum)根尖为材料,用HU-APM双阻断法对细胞有丝分裂中期同步化和前、后、末期部分同步化作了探讨,表明在1.25 mmol/LHU, 4 μmol/APM处理可使细胞有丝分裂中期指数(Met. I)达35%, 18hr的HU单独处理可检测到27%的前期分裂细胞。HU对后、末期的部分同步化也有明显作用,约有17%的后、末期细胞被检测到。用Schubert介绍的方法,分离出中期染色体,每微升悬浮液中含170条染色体。

关键词: 同步化, 中期指数, 大蒜, 羟基脲, 甲酰氨草磷

Abstract: In this study, metaphase cell synchrony and prophase, anaphase-telophase partial synchrony during mitosis of root tip cells in Allium sativum have been studied by using hydroxyurea (HU) and amiprophosmethyl (APM) treatment. It was shown that metaphase index (Met. I) can be increased around 35% at 1.25 mmol/L HU plus 4 μmol/L APM treatments in root tip cells. About 27% prophase cell division can be detected when root tips treated for 18hr with 1.25 mmol/L HU. Partial synchronization of cell division at anaphase-telophase has also been obtained only using HU treatment, mitotic frequency has reached at 17%.
Metaphase chromosomes isolating from root tip cells was performed using Schubert method, about 170 chromosomes were obtained in per-microlitre of the isolation buffer.

Key words: Synchronization, Metaphase index, Allium sativum, Hydroxyurea, Amiprophosmethyl

彭永康, 赵建, 陈瑞阳. 大蒜根尖细胞有丝分裂同步化诱导与中期染色体分离[J]. 植物研究, 1999, 19(3): 302-307.

Peng Yong-kang, Zhao Jian, Chen Rui-yang. INDUCTION OF SYNCHRONY AND ISOLATION OF METAPHASE CHROMSOMES FROM MITOTIC ROOT TIP CELLS IN ALLIUM SATIVUM[J]. Bulletin of Botanical Research, 1999, 19(3): 302-307.

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