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植物研究 ›› 1998, Vol. 18 ›› Issue (1): 90-96.

• 论文 • 上一篇    下一篇

植物微核技术在监测水质污染中的应用

刘二曼, 邹莉, 刘清臻, 刘宏伟   

  1. 东北林业大学, 哈尔滨 150040
  • 收稿日期:1997-03-10 出版日期:1998-03-15 发布日期:2016-06-14

APPLICATION OF PLANT MICRONUCLEUS TEST TECHNIQUE IN DETECTING WATER POLLUTION

Liu Er-man, Zou Li, Liu Qing-zhen, Liu Hong-wei   

  1. Northeast Forestry University, Harbin 150040
  • Received:1997-03-10 Online:1998-03-15 Published:2016-06-14

摘要: 应用韭菜微核技术,检测哈尔滨市量具刃具厂含铬废水的遗传毒性,试验表明:含铬废水可诱发韭菜和蚕豆根尖细胞产生微核,进行回归分析,其蚕豆对Cr+6诱变剂回归分析为:y=24.94+127.77x(r=0.996, P<0.01),韭菜对Cr+6诱变剂回归方程为:y=21.74+100.23x(r=0.900,P<0.01),韭菜对含铬废水的回归方程为:y=11.17+3.83x(r=0.781, P<0.05),韭菜根尖微核技术具有微核效应良好,方法简单,取材容易等优点。

关键词: 微核技术, 含铬废水, 蚕豆, 韭菜

Abstract: With plant micronucleus test technique, the genetic toxicity of chromium-borne waste water, which is taken from Harbin Measuring Instrument and Cutting Tolls Mill, has been studied.Its result shows that the micronucleus can be, have been, induced by chromium-borne waste water from root-tip cell of Allium tuberosum Roxb.and Vicia faba L.The regression equation between the plants and Cr+6-inducer is as follows:y=24.94+127.77x(r=0.996, P<0.01) for Vicia faba L.and y=21.74+100.23x(r=0.900, P<0.01) for Allium tuberosum Roxb.It also is Y=11.17+3.83x(r=0.781, P<0.05) between Allium tuberosum Roxb.and chromium-borne waste water.A simple method, being easy of collecting materials, and a good micronucleus effect are shown by micronucleus test technigue on the root-tip cell of Allium tuberosum Roxb.

Key words: Micronucleus Test Technique, Chromium-borne waste water, Vicia faba L.Allium tuberosum Roxb.