雨堡组织培养的研究

植物研究 ›› 1996, Vol. 16 ›› Issue (1): 140-145.

雨堡组织培养的研究

朱虹, 云希和, 朱泳, 崔占武

东北林业大学, 黑龙江, 哈尔滨 150040

收稿日期:1995-11-01 出版日期:1996-03-15 发布日期:2016-06-14

THE TISSUS CULTURE OF REGENSBERG

Zhu Hong, Yun Xi-he, Zhu Yong, Cui Zhan-wu

Northeast Forestry University, Harbin 150040 Heilongjiang

Received:1995-11-01 Online:1996-03-15 Published:2016-06-14

摘要/Abstract

摘要： 取雨堡当年生的枝条上饱满的未萌发的侧芽为外植体,培养于M S培养基上,其中附加各种不同种类及浓度的激素。在附加6-BA1.0mg/l的M S培养基上,诱导芽的效果最好;在附加6-BA1.0+NAA0.05+ZT0.1mg/l的M S培养基上,芽的增殖效果最佳;在附加KT0.3+NAA0.05+ZT0.1mg/l或6-BA0.3+NAA0.05+ZT0.1mg/l的MS培养基上,最有利于壮苗;在1/2MS培养基中,附加IBA0.5+NAA0.3ml/l或IAA0.5+NAA0.3mg/l的培养基中,生根效果最佳。试管苗高2-3cm,且具有4-8条短根时,开瓶煅炼3-5天,移入培养土中生长的效果好,种植在保温保湿环境中,试管苗成活率高。

关键词: 雨堡, 细胞分裂素, 生长素

Abstract: The tissue culture of Regensberg with the tip of shoots as the explants was experimnented in MS medium with addition of different contentration and kinds of multiplication.The results show that sprouting of bud was best on MS medium with addition of 6-BA 1.0mg/l;and the multiplication of adventitious buds was best on MS medium with the addition of 6-BAl.o+NAA0.05mg/l;and vigorous and healthy bud was best on MS mdeuim with addition of KT0.3+NAA 0.05+ZT0.1mg/lor6-BA0.3+NAA0.05+ZT0.1mg/l;and the growth of roots was best on 1/2 MS medium with the addition IBA0.5+NAA0.3mg/lor IAA0.5+NAA0.3mg/l.It was better to transplant from test-tube grew to 2-3 cm high and 4-8roots and after the plantlets stayed 3 or 5 days without cap of test-tube in the experimental. The plantlets had better percentage of survival when it had the situation with deeping degree of temperature and humidity.

Key words: Regensberg, Cytokinin, Auxin

朱虹, 云希和, 朱泳, 崔占武. 雨堡组织培养的研究[J]. 植物研究, 1996, 16(1): 140-145.

Zhu Hong, Yun Xi-he, Zhu Yong, Cui Zhan-wu. THE TISSUS CULTURE OF REGENSBERG[J]. Bulletin of Botanical Research, 1996, 16(1): 140-145.

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