软枣猕猴桃悬浮细胞系的建立及其影响因素

植物研究 ›› 1991, Vol. 11 ›› Issue (3): 77-83.

软枣猕猴桃悬浮细胞系的建立及其影响因素

张喜春¹, 吴绛云²

1. 150040 哈尔滨, 东北农学院生物工程系;
2. 150030 哈尔滨, 东北农学院生物工程系

出版日期:1991-09-15 发布日期:2016-06-14

ESTABLISHMENT OF SUSPENSION CELL LINE OF GOOSEBERRY AND INFLUENCEABLE FACTORS

Zhang Xi-chun¹, Wu Jiang-yun²

1. Department of forestry, Northeast Fotestry University, Harbin 150040;
2. Department of bioengineering, Northeast Agriculture College, Harbin 150030

Online:1991-09-15 Published:2016-06-14

摘要/Abstract

摘要： 以软枣猕猴桃(Actinidia arguta Planch)叶片为试材,以MS为基本培养基,附加生长素(2.4-D、NAA)和玉米素(ZT)成功地诱导出愈伤组织,结果表明:软枣猕猴桃叶片在MS+ZT 1mg/1+2.4-D3mg/1的情况下能诱导出适合于建立悬浮细胞系的愈伤组织。并研究了愈伤组织生长年龄、激素浓度、肌醇和蔗糖浓度四个方面对建立悬浮细胞系的影响。表明愈伤组织以生长10天时接种活细胞率最高;以MS培养基附加ZT1mg/1+2.4-D3mg/1较好;肌醇以200mg/1圆形细胞频率最高;蔗糖以5%最适。

Abstract: The leaves of Actinidia arguta Planch were cultured on MS basic med-ium supplemented with 2.4-D or NAA and zeatin in vitro. The callus wereinduced successfullg. The results have been obtained as follows:the callus fitting for the establishment of cell line coulb be obtained from leaves of Act-inidia arguta Planch on MS+ZT1mg/l+2.4-D3mg/l. Tbe highest cell surv-ival rate has been obtained from callus that had been cultured for ten days. The satisfied result have beenobtained on MS+ZT1mg+2.4-D3mg/l.The high-est frequency of round cell is medium with 200mg/l myo-inositol. Suitable concentration of sucrose is 5%.

张喜春, 吴绛云. 软枣猕猴桃悬浮细胞系的建立及其影响因素[J]. 植物研究, 1991, 11(3): 77-83.

Zhang Xi-chun, Wu Jiang-yun. ESTABLISHMENT OF SUSPENSION CELL LINE OF GOOSEBERRY AND INFLUENCEABLE FACTORS[J]. Bulletin of Botanical Research, 1991, 11(3): 77-83.