关键词: 香雪兰, 花色, 花色苷, 液质联用

Abstract: We analyzed the anthocyanin profiles in 6 cultivars of freesia(Freesia hybrida). The petal color was observed according to the Royal Horticultural Society Color Card(RHSCC). Petal pigment type was identified by characteristic color reaction. UPLC-PAD and UPLC-Q-TOF-MS were used for qualitative and quantitative analysis of anthocyanins. The flavonoids in petals of all cultivars were detected, while carotenoids were not detected; meanwhile, every cultivar, except for ‘Shangnong Ruxiang’, contained anthocyanins in petals. Among five anthocyanincontained cultivars, the highest anthocyanin content was observed in ‘Shangnong Purple Rose’, followed by ‘Shangnong Chenghong’. Totally 6 anthocyanin components were detected in petals of these cultivars, including Delphinidin 3,5-O-glucoside, Petunidin 3,5-O-glucoside, Delphinidin 3-O-glucoside, Malvidin 3,5-O-glucoside, Petunidin 3-O-glucoside and Malvidin 3-O-glucoside. Three anthocyanin components were detected in petals of ‘Shangnong Zixueqing’ and ‘Shangnong Gongfen’, of which were majorly malvidin and petunidin derivated anthocyanins. Malvidin-anthocyaninswith single or double glucosides was present in petals of ‘Shangnong Zixueqing’, while all anthocyanins in petals of ‘Shangnong Gongfen’ bonded with double glucosides, such as Delphinidin 3,5-O-glucoside, Petunidin 3,5-O-glucoside and Malvidin 3,5-O-glucoside, major Petunidin 3-O-glucoside and minor Delphinidin 3-O-glucoside in petals of ‘Shangnong Purple Rose’. While only one anthocyanin component was detected in petals of ‘Shangnong Golden Queen’ and ‘Shangnong Chenghong’, which was Delphinidin 3,5-O-glucoside and Malvidin 3,5-O-glucoside, respectively.

Key words: freesia, flower color, anthocyanin, UPLC-MS