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植物研究 ›› 2007, Vol. 27 ›› Issue (4): 416-420.doi: 10.7525/j.issn.1673-5102.2007.04.009

• 论文 • 上一篇    下一篇

高温胁迫对花生幼苗光合速率、叶绿素含量、叶绿体Ca2+-ATPase、Mg2+-ATPase及Ca2+分布的影响

宰学明1,3;钦 佩1*;吴国荣2;王 光1;闫道良1   

  1. 1.南京大学生命科学学院,南京 210093 2.南京师范大学生命科学学院,南京 210097 3.金陵科技学院园艺系,南京 210038
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-07-20 发布日期:2007-07-20
  • 通讯作者: 钦 佩
  • 基金资助:
     

Effects of High Temperature Stress on Photosynthesis Rate, Contents of Chlorophyll, Ca2+-ATPase,Mg2+-ATPase in Chloroplast and Ca2+ Distribution in Peanut Seedlings

ZAI Xue-Ming;QIN Pei;WU Guo-Rong;WANG Guang;YAN Dao-Liang   

  1. 1.School of Life Sciences,Nanjing University,Nanjing 210093 2.School of Life Sciences,Nanjing Normal University,Nanjing 210097 3.Horticulture Dept.,Jinling Institute of Technology,Nanjing 210038
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-07-20 Published:2007-07-20
  • Contact: QIN Pei
  • Supported by:
     

摘要: 将水培后盆栽的花生幼苗,置于培养箱42℃高温培养,定时测定幼苗叶光合速率、叶绿素含量和叶绿体Ca2+-ATPase、Mg2+-ATPase的相对活性,并观察幼叶细胞内Ca2+分布的变化。试验结果表明:高温胁迫过程中,光合速率及叶绿素含量都随处理时间的延伸而下降,并呈显著正相关;叶绿体Ca2+-ATPase和Mg2+-ATPase高温胁迫过程中相对活性呈先升后降趋势,Ca2+-ATPase热敏性高于Mg2+-ATPase;高温胁迫过程中,Ca2+具有从胞外转运到胞质内和叶绿体中的趋势,Ca2+能够稳定高温胁迫下叶肉细胞膜和叶绿体的超微结构。

关键词: 高温胁迫, 花生幼苗, 光合速率, 叶绿素, 叶绿体, Ca2+-ATPase, Mg2+-ATPase, Ca2+分布

Abstract: Peanut seedlings potted after tank farming were cultured at 42℃ in incubator, and the photosynthesis rate, contents of chlorophyll, activities of Ca2+-ATPase, Mg2+-ATPase in chloroplast were measured regularly and the Ca2+ distribution in young leafage was observed accordingly. The results show that under high temperature stress, the photosynthesis rate and contents of chlorophyll decreased, and were positively correlated. Moreover, activities of Ca2+-ATPase, Mg2+-ATPase in chloroplast increased early and then decreased later, and the sensitivity to heat of Ca2+-ATPase was higher than Mg2+-ATPase. In addition, most of Ca2+ was transferred to cytosol and chloroplast across the plasmalemma, and Ca2+ could stabilize the structures of membrane and chloroplast under high temperature stress.

Key words: high temperature stress, peanut seedlings, photosynthesis speed, chlorophyll, chloroplast, Ca2+-ATPase, Mg2+-ATPase, Ca2+ distribution

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