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    15 March 1998, Volume 18 Issue 1
    SOME REVISIONS AND SUPPLEMENTS ON CARYOPHYLLACEAE FROM CHINA
    Lu De-quan
    1998, 18(1):  1-3. 
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    In order to compile the Flora of China, have revised Caryophyllaceae of China(exclude Arenaria, Silene, and Stellaria).Two specific names are merged as synonyms, 9 species, 2 subspecies, and 1 variety are supplemented.At the same time, some names and literatures of genus and species are corrected.
    NOTES ON THE SCIENTIFIC NAMES OF SEVERAL SPECIES OF LEGUMINOSAE AND LAURACEAE IN CHINA
    Huang Pu-hua
    1998, 18(1):  4-8. 
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    In process of writing the 《Flora of China》, based on specimens and Iiteratures examined, the classification of several species of Leguminosae and Lauraceae in China is revised.Five new combinations, four new synonyms and reinstatment of one specific name are made here in present paper.Five new combinations are:Desmodium strigillosum var.pendenticarpum(C.Z.Gao et Q.R.Lai) P.H.Huang, Desmodium flexuosum var.cordifoliolatum(P.C.Li) P.H.Huang, Podocarpium densum(C.Chen et X.J.Cui) P.H.Huang, Podocarpium podocarpum var.mandshuricum(Maxim.) P.H.Huang and Podocarpium podocarpum var.japonicum(Matsum.) P.H.Huang.Four new synonyms are as follows:Litsea lii C.E.Chang and var.nunkao-tahangensis(Liao) Liao are recognized as new synonym of Litsea pseudoelongata Liou, L.akoensis var.chitouchiaoensis Liao is recognized as a new synonym of Litsea hayatae Kanehira and Desmodium densum(C.Chen et X.J.Cui) H.Ohashi is recognized as a new synonym of Podocarpium densum(C.Chen et X.J.Cui) P.H.Huang.Litsea taiwanian a Kamikoti is a legitimate name of reinstatment because Litsea pedicellata is preocupied.
    TWO NEW VARIETIES FROM SHANDONG
    Zhu Ying-qun, Zang De-kui, Du Ming-yun, Sun Yu-gang, Yan Da-cheng
    1998, 18(1):  9-9. 
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    A NEW VARIETY OF DIANTHUS FROM NORTHEAST OF CHINA
    Zhang Gui-yi, Yuan Xiao-ying
    1998, 18(1):  10-11. 
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    NEW FLAGELLATAE FROM HARBIN, CHINA
    Zhang Yue
    1998, 18(1):  12-14. 
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    NEW RECORDS OF ACROCHAETIACEAE FROM CHINA,IV
    Luan Ri-xiao, Zhang Shu-mei
    1998, 18(1):  15-19. 
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    This paper is continued 6 species Acrochaetiaceae new to China i.e.(1) Audouinella bonnemaisoniae, (2) A.dictyotae, (3) A.drewii, (4) A.netrocarpa, (5) A.pacilica, (6) A.vaga
    ON THE FLORA OF SEED PLANTS IN GUANDI MOUNTAINS,SHANXI
    Zhang Feng, Shangguan Tie-liang, Zheng Feng-ying
    1998, 18(1):  20-27. 
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    Guandi Mountains, located in the middle of Luliang Ranges, is situated between 37°20'~38°20'N latitude and 110°18'~111°18'E longitude in western Shanxi.There is a typical warm-temperate continental monsoon climate in the mountains, which is cool and arid in winter, however, warm and rainy in summer.It is now known that there are 737 species of seed plants, belonging to 335 genera and 85 families in Guandi Mountains, among which temperate distribution compositons make significant dominant.It has 249 genera and make 85.67% in total genera(excluding cosmopolitan).Based on the analysis of the flora of species and genera and constructive species and dominant of the vegetation in Guandi Mountains, it is indicated that the flora has some typical characteristics of warm-temperate deciduous broadleave forest.Compared with the flora and the vegetation of Taiyue Mountains, the southern segment of Taihang Mountains in Shanxi and Taibai Mountains, Qinling Ranges in Shanxi, there is higher homogeneitive and closely relationship between Guandi Mountains and the southern segment of Taihang Mountains; however, there is some significant differences between Guandi Mountains and Taibai Mountains, Qinling Ranges.
    STUDIES ON THE SPORE MORPHOLOGY OF ALEURITOPTERIS FEE IN BEIJING
    Liu Jia-xi
    1998, 18(1):  28-30. 
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    species belonging to Aleuritopteris Fee spores are examined under SEM and LM.The morphological characteristic of each species are described.And the differences among the spores of these species are discussed.It seems to contibute to the materials for systematic and palynology of Aleuritopteris Fee.
    OBSERVATION OF THE FRUITS OF LACTUCA SATIVA AND ITS VARIETIES WITH AN ELECTRON MICROSCOPE
    Shen Xian-sheng, Lu Run-long
    1998, 18(1):  31-33. 
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    The scanning electron microscope observation was given for the achenes of Lactuca sativa, L.sativa var.angustata, L.sativa var.romana and L.sativa L.var.yemensis X.S.Shen et R.L.Lu which was reported as a new variety in this paper.The stem and leaves of the new variety are covered with white wax powder.Its vascular tissue is not developed very well.The stem is even from base to top.The structure of the leaf is isobilateral.The obovate-elliptic achene possesses sparsely 8-12 rhaphes, and part of them are disappear in the top of the achene.
    DEVELOPMENTAL AND ANATOMICAL STUDIES ON THE FLORAL NECTARIES IN ELAEAGNUS ANGUSTIFOLIA
    Deng Yan-bin, Liu Zhong-yuan, Jiang Yan-cheng, Wang Hong
    1998, 18(1):  34-37. 
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    Anatomic studies and cytological observation of the floral nectary in Elaeagnus angustifolia L.were investigated with light and scanning electron microscopy, Elaeagnus angustifolia of floral nectary located on the tubular disc in the base of the style, it belongs to disc nectary.The nectary located on the outside of the disc, which is composed of epidermis and nectariferous tissue.The cuticula and modified stomata are present in the epidermal layer.During the development of nectariferous tissue, both the vacuole and the starch grains occurred regularly decrease and increase change.Finally, the nectar secreted from the stoma on the surface of the disc nectary.
    EMBRYOLOGICAL STUDIES IN RHODIOLA SACHALINENSIS A. BOR
    Zhang Ping, Shen Jia-heng
    1998, 18(1):  38-45. 
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    Abstract This paper reported the studies of overall embryology of Rhodiola sachalinensis A.Bor..Development of the anther wall followed the Basic type.Part of the tapetal cells divided into regular two layers.Tapetal cells was uninucleate or binucleate, and blonged to the secretory type.Meiosis of microspore mother cell was normal and cytokinesis was of the simultaneaus type.The tetrad was tetrahedral in shape.The mature pollen grains were 2-celled.The ovule was anatropous, bitegminous, crassinucellate and nucellar beak was formed during the development of the ovule.The megaspore mother cell went meiosis to form a linear tetrad.The chalazal megaspore was functional.The development of the embryo sac was conformed as the Polygonum type.In mature embryo sac, the nuclei of the egg cell and synergids located in the chalazal part of the cells and the microplar part of the cells possessed vacuoles; two polar nucleus fused into the secondary nucleus, and located near the chalazal end of egg cell; three antipodal cells degenerated.The fertilization belonged to the premitotic type of syngamy; the development of the endosporm belonged to the cellular type.The first division of endosperm cell given rise to two cells, the chalazal cell developed into haustorioum without division.The development of the embryo belonged to the Caryophyllad type.In 2-celled proembryo basic cell became much enlarged and formed sacciform haustorium which invade the micropylar.
    COMPARATIVE STUDIES OF ANATOMICAL ECOLOGY BETWEEN ADENOPHORA LOBOPHYLLA AND A. POTANINII
    Zu Yuan-gang, Zhang Wen-hui, Yan Xiu-feng, Wu Shuang-xiu, Zhou Fu-jun
    1998, 18(1):  46-54. 
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    By the light and scanning electron microscope, the anatomical characteristics of the root, the rhizome, the stem, the seed and the leaf between Adenophora lobophylla Hong.endangered species, and A.potaninii, Korsh.extentive species, have been observed and compared.The results show that two species have obvious difference in anatomical characteristics of the leaf and the stem.The anatomical characteristics demonstrate that A.potaninii has stronger adaption to dry river valley, while A.lobophylla has the poorer ability to adapt the locative environment.
    THE STUDY ON RELATIONSHIP OF LARCHES IN NORTHEAST CHINA BY RAPD
    Shi Fu-chen, Kazuo Suzuki, Hiromitsu Kisanuki
    1998, 18(1):  55-62. 
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    RAPD is taken to study the relationship of larches in Northeast of China.41 primers are selected from 100 primers.120 Loci show polymorphism.UPGMA is taken to calculate the genetic distance within and between populations.Systematic tree is constructed.The results prove that there is difference between larches in Daxingan Mountain, Xiaoxingan Mountain, and larches in Changbai Mountain.But the genetic distance between them doesn't reach the standard of species.It is more reasonable to regard larches in Changbai Mountain as a variaty of Larix gmalinii namely, Larix gmelinii var.olgensis Ostenf.et syrach larsen.
    STUDY ON POLLINATION BIOLOGY IN 10 SPECIES OF LILIUM
    Yang Li-ping, Zhang Xiao-fang, Gao Yi-ke
    1998, 18(1):  63-67. 
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    Self-pollination and interspecific-pollination were studied in 10 species of Lilium.The result shows that in L.d istichum, L.concolor var.pulchellum, L.dauricum and L.regale self-pollination is sterility.Affinity of cross-pollination in one plant is better than self-pollination in L.davidii and L.callosum.Affinity in interspecific-pollination is different sith norphology with convention.Thirteen crossing groups were researched.In same group, after reciprocal crosses of L.pumilum and L.cernum, we can get 16% and 25% albuminous seed, L.callosum×L.regale, we can get 12% albuminous seed, that it germination rate is 64%.In another group, reciprocal crosses of L.dauricum and L.concolor var.pulchellum, had not seed.In L.distichum×L.regale, we can get 82% albuminous seed, that it germination rate is 85%.
    COMPARATIVE ANALYSIS OF SOLUBLE PROTAINS IN D2 TYPE PCMS LINES OF WHEAT ON DIFFERENT PHOTOPERIODIC CONDITIONS
    Jiang Jing, Liu Wei-hua, Shi Rui, Zhang Yue Xue, Li Ji-lin
    1998, 18(1):  68-72. 
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    The combination of D2 type cytoplasm and certain nucleus can cause wheat to be sensitive to long-day condition(≥ 15 hours)with the expression of pistillody of stamens and pollen abortion.Observation of tranverse sections using the scanning eletron microscope revealed that pistillate stamens contained ovule-like structures but had no ovule tissue、tapetal cells and pollen grains.Comparison was made using unidirectional SDS-PAGE technique among soluble proteins of chloroplast、mitochondria and cytoplasm derived from wheat(c)-N26(D2 type cytoplasm) and wheat N26(B type cytoplasm, the same nucleric genome).The SDS-PAGE maps of soluble proteins of chlorplast、mitochondria and cytoplasm derived from(c)-N26 under a short-day condition(≤ 14.5hours)showed no difference to that of N26 under a long-day condition.But there were distinct differences between SDS-PAGE maps of(c)-N26 cultured on the long-day condition and short-day condition respectively.The trends is the reduction of proteins.So it can be infered from the facts above that on the long-day condition pistillody of stamens in D2 PCMS line is probably relation to repression of genes expression in nuclei、chloroplast and mitochondria.
    FLOUR QUALITY OF NEAR-ISOGENIC LINES OF HMW GLUTENIN SUBUNITS 2+12 AND 5+10 IN CULTIVAR LONGMAI 15
    Zhang Yan-bin, Xiao Zhi-min, Qi Shi-yu, Lan Jing, Zhao Nai-xin
    1998, 18(1):  73-77. 
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    Near-isogenic lines of HMW glutenin subunits 2+12 and 5+10 of cultivar Longmai 15 were obtained by analysis of morphology, SDS-PAGE and A-PAGE.The Zeleny sedimentation of Longmai 15 with 5+10 subunits is 4.5ml(12%) higher than Longmai 15 with 2+12 subunits.Ratio of Zeleny sedimentation and wet gluten is increased from 1.08 to 1.32.The results indicated that 5+10 subunits is better than 2+12 subunits in flour quality.
    EFFECTS OF PENICILLU M CITRINU M ELICITOR ON THE BIOSYNTHESIS OF TAXOL IN SUSPENSION CELL CULTURES OF TAXUS CHINENSIS
    Li Jia-ru, Liu Man-xi, Cao Meng-de, Wang Jun-jian
    1998, 18(1):  78-82. 
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    The Penicillum citrinum elicitor was extraxted after autoclaveing a 6~7-day old culture of P.citrinum which could promote taxol biosynthsis in suspension cell cultures of Taxus chinensis.The optimum elicitor cocentration was found to be 50μg carbohydrate/ml medium of T.chinensis cell cultures. T.chinensis cells showed strongest response to the elicitor during the growth phase(20d).The time of autoclaving(20~90min) did not affect the elicitor's activity.
    THE APPLICATION OF PEROXIDASE ISOENZYME ON SPEICES SOURCE DIVISIONS OF PINUS SYLVESTRIS VAR. MONGOLICA
    Man Xiu-ling, Cao Ji-fu, Yao Yu-jun
    1998, 18(1):  83-89. 
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    Based on electric swimming.The peroxidase isoenzyme spectrums of pinus sylvetris were gained and done by variance analysis, main-composition analysis and gathering analysis.The results showed the species sources were divided into twe big species source areas which were sand soil species source respresented by Aer Mountain and Handagai and mountain region species source respresented by Gold Mountain and Ta river, and four small independent species source area respresented by Me river, Tuqiang, Honghuaerji and Kalun Mountain.This result was consistent with that divided by meteorological factors.
    APPLICATION OF PLANT MICRONUCLEUS TEST TECHNIQUE IN DETECTING WATER POLLUTION
    Liu Er-man, Zou Li, Liu Qing-zhen, Liu Hong-wei
    1998, 18(1):  90-96. 
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    With plant micronucleus test technique, the genetic toxicity of chromium-borne waste water, which is taken from Harbin Measuring Instrument and Cutting Tolls Mill, has been studied.Its result shows that the micronucleus can be, have been, induced by chromium-borne waste water from root-tip cell of Allium tuberosum Roxb.and Vicia faba L.The regression equation between the plants and Cr+6-inducer is as follows:y=24.94+127.77x(r=0.996, P<0.01) for Vicia faba L.and y=21.74+100.23x(r=0.900, P<0.01) for Allium tuberosum Roxb.It also is Y=11.17+3.83x(r=0.781, P<0.05) between Allium tuberosum Roxb.and chromium-borne waste water.A simple method, being easy of collecting materials, and a good micronucleus effect are shown by micronucleus test technigue on the root-tip cell of Allium tuberosum Roxb.
    STUDY ON REGIONALIZATION OF ECOLOGICAL ENVIRONMENTAL FUNCTION IN HEILONGJIANG NATURAL RESERVE
    Zhao Shu-fen, Guo Ya-sheng, Jia Li, Han Yu-wu
    1998, 18(1):  97-106. 
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    Natural protection is the heat topic concerned by all over the world.In rccent ycars, the types, distributions and quanlities are increasing, with the building of Natural Reserve in Heilongjiang province.But there are still many problems of administration and development in Natural Reserve, such as contradictoriness between economic development and natural protection, etc.According to the features and problems of different type Natural Reserve, 24 provinicial and national Natural Reserves have been studies, and the ecological environmental function were regionalized.In order to help Natural Reserve development and administration, protective suggestions are put forward in the paper.
    GENUS ANTROPHYUM KAULF. FROM CHINA AND NEIGHBORING REGIONS
    X. C. ZHANG
    1998, 18(1):  107-117. 
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    Nine species of Antrophyumare recog nized from China, Vietnam, and Japan, with critical notes on the taxonomy of some species from mainland of Asia.Asubdivision of three groups based on the morphology of paraphy ses interspersed between the sporangia is proposed without formal nomenclature pending future monog raphic studies
    STUDY ON THE INDIVIDUAL GROWTH DYNAMIC OF ADENOPHORA LOBOPHYLLA——GROWTH DYNAMIC IN ONE GROWING SEASON
    Zhang Wen-hui, Zu Yuan-gang
    1998, 18(1):  118-127. 
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    In the fixed sample plot at different habitats, the growing process of an endangered species, Adenophora lobophylla Hong population was observed.The regulation of the adult individual growth and phenological characteristics was studied.The relation between time(x, day) and the total growth of biomass above ground(y, g) could be expressed:y=0.2872-0.0187x+0.0009x2, and the relations between time(x, day) and the growth of biomass(y, g) of every organs such as branch, leaf, flower branch, flower bud, flower and fruit could be expressed by the general equation:y=b0+b1x+b2x2.During the time of rapid growth from April 10 to August 15, the relation between time(A, day) and the growth of total biomass of all organs above ground(B.g) could be expressed by Logistic equation:B=0.99/(1+e^(3.613-0.149A)); the relations between time(A, day) and the growth of biomass(B, g) of stem, flower branch could be expressed:B=K/(1+e^(c-rm.A)).The regulation of growth of individual in the deciduous thorny shrub community were compared with that in grass(drought-resistant) community.The individual in the open grass community begins its growth(phenology) later and ends its growth earlier than that under shrub community.
    STUDY ON THE INDIVIDUAL GROWTH DYNAMIC OF ADENOPHORA LOBOPHYLLA——THE ROOT SYSTEM GROWTH DYNAMIC IN THE LIFE SPAN
    Zhang Wen-hui, Zu Yuan-gang
    1998, 18(1):  128-136. 
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    In the distribution area, Jinchuan county, of Adenophora lobophylla Hong.population, 29 sample plots were investigated.The root system spatial structure and biomass of 1502 individuals with different ages were studied, and 5 populations at different altitudes were compared.The environmental factors which influence the root system development were analyzed too.The root system of the population at altitude of 2700-2900m could develop fully, the biomass and size of taproot, lateral root, secondary lateral root, thin root all reach the maximum.If the habitat is higher or lower than this altitude, the root systems of the population could not develop well.The most principal reason for the underdevelopment at the lower altitude was assumed to be drought and external extreme disturbance, and at the higher altitude was assumed to be low temperature.The relation between root system biomass growth(y, g) and ages(x, year) could be expressed by the equation:y=0.003783x1.7953 for the whole population, and for the other 5 populations from altitude of 2300m to 3400m could be expressed by the general equation:y=a·xb.