Sugar, phytohormone, tannins and flavonoids changes during the growth and development of Eupatorium adenophorum
ZU Yuan-Gang;CHEN Hua-Feng;WANG Wen-Jie;JIA Jing;ZHU Lei;ZHANG Nai-Jing
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The differences of sugar,phytohormone,tannins and total flavonoids in Eupartorium adenophorum at different developmental stage were studied,employing the HPLC and the spectrophotometric methods. The results showed that: the content of glucose is the highest in the sprout (6.93%),however,in the seed (0.16%),seedling (1.27%),adult (1.07%) are obviously lower than that at the stage of sprouts. Alike,the content of sucrose is rather high in sprout which is as high as 2.68%,and followed by in seed (0.32%),seedling (0.52%),adult (0.09%). The maximum fructose is also observed in the sprout stage (3.28%) and followed by seedling stage (2.56%),seed (0.14%) and adult stage (0.55%). The content of fructose,raffinose,mannose differs significantly,i.e. these three kinds of sugar are detectable only at seedling stage(respectively 0.04%,0.04% and 0.03% ) but no obvious difference. At the developmental stage of E. adenophorum,the content of IAA at the stages of sprout (14.20μg·g-1 Dw) is 1.25μg·g-1 Dw higher than that in seed,while it is respectively 1.63 times,5.55 times higher than that of seedling and adult. GA is also varied with developmental stage. The content of GA at seedling stage (901.11μg·g-1 Dw) is obviously higher than those of adult (280.50μg·g-1 Dw),sprout (97.35μg·g-1 Dw) and seedling (84.29μg·g-1 Dw). Difference of the change of tannins is obvious in development of E. adenophorum. The tannins content of seed is obviously higher than that of the sprout,seedling and developmental stages (<0.03%) by 1.98%; The content of total flavonoids increases linearity during the developmental progress. The content of flavonoids in seed is as low as 0.04%,while much higher values are observed at the sprout (0.60%),seedling (0.64%) and adult (0.62%) stages and there are no differences among these three stages. Our results indicate that the content of sugar,phytohormone,tannins and total flavonoids is in correlation to the basic rule of plant growth and development,also to regulation in metabolism process.