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    20 November 2010, Volume 30 Issue 6
    Two New Species of Yulania Spach from Hubei Province of China
    FU Da-Li;ZHAO Tian-Bang;CHEN Zhi-Xiu;CHEN Shu-Sen
    2010, 30(6):  641-644.  doi:10.7525/j.issn.1673-5102.2010.06.001
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    Two new species of the genus Yulania Spach of Magnoliaceae were described from Hubei Province of China. They are Yulania verrucata D. L. Fu,T. B. Zhao et S. S. Chen, sp. nov. and Y.cuneatofolia T. B. Chao, Z. X. Chen et D. L. Fu,sp. nov.. The main particular distinguishing features of Y.verrucata are that the branches, leaf backs, petioles, stipules and the pedicels of flower and fruit are glabrous and the lenticels are narrow, brown and verruciform ellipsoids on twigs. Those of Y.cuneatofolia are that the leaves are cuneiform and the petals are rugate.
    A New Species of Ilex from Hunan Province
    SHANG Chih-Bei;TANG Kai-Shan;DU Duo-Qing
    2010, 30(6):  645-647.  doi:10.7525/j.issn.1673-5102.2010.06.002
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    A new species of the genus Ilex L. of Aquifoliaceae, Ilex urceolatus C.B. Shang, K. S. Tang et D. Q. Du, was described in this paper. The species appears to be similar to I.hylonoma Hu et Tang and I.centrochinensis S. Y. Hu, but it has some particular characteristics which can be distinguished from I.hylonoma Hu et Tang and I.centrochinensis S. Y. Hu. The main destinguishing features are that the margin of leaves waved, with 10-19 pairs of sharp spines, the fruit bigger, 9-12 mm wide, 11-17 mm long, urceolate, never globose or subglobose. The species was found and collected on November 8, 2009 in Liping village, Lianyuan city, Hunan Province of China by Du Duo-qing and Tang Kai-shan. The Holotype is reserved in Herbarium of Nanjing Forestry University (NF).
    A new variety of Pulsatilla(Ranunculaceae) from Greater Khingan Mountains of Inner Mongolia
    YU Jing-Hua;YUAN Shu-Sheng;TONG Lu
    2010, 30(6):  648-648.  doi:10.7525/j.issn.1673-5102.2010.06.003
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    This paper discribed a new variety of Ranunculus asiatious L. of PulsatillaPulsatilla turczaninovii Kryl. et Serg. var. fissasepalum and drew the picture of morphology. The main difference between this variety and P.turczaninovii, the old variety, is that the sepals of outer ring was bipartite in different depths and sizes. The middle lobe is the biggest and the size of the bilateral lobes is not exactly the same.
    A New Variety of the Genus Chrysanthemum L. from China
    ZHOU Jie;CHEN Jun-Yu
    2010, 30(6):  649-650.  doi:10.7525/j.issn.1673-5102.2010.06.004
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    Chrysanthemum vestitum(Hemsl.)Stapf. var. latifolium J. Zhou et J. Y. Chen, a new variety of the genus Chrysanthemum L. from China is reported. The difference between the new variety and the original species is that the variety is with fewer branches,having orbicular and ovoid leaves,4 to 7 centimeters long,3 to 5 centimeters wide.Diameters of its flower heads are larger,being about 4.5 to 5.0 centimeters.The new variety mainly distributes in the western slopes of Dabie Mountains in Anhui province.The species grows more vertically and with more branches,having lanceolate or spatulate leaves narrower than those of the original species.The leaves are about 4 to 6 centimeters long and 2 to 3 centimeters wide.Diameter of capitulum is about 3.5 to 4.5 centimeters,being smaller than that of the original species.The species mainly distributes in Hubei province and Funiu Mountains in Henan province.
    New records of Chrysophytes in Shanxi Province,China
    FENG Jia;XIE Shu-Lian*
    2010, 30(6):  651-659.  doi:10.7525/j.issn.1673-5102.2010.06.005
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    Based on the study of specimens and literature, 34 species and 1 variety in 15 genera and 6 families of Chrysophytes are reported in Shanxi Province for the first time. The identification features, characteristics of the habitat and distribution of each species are given. The specimens are deposited in the herbarium of Shanxi University(SXU).
    Revision of Swietenia mahagoni(Meliaceae) in Chinese Taxonomic Literature
    LIU Hai-Sang;CHI Min-Jie
    2010, 30(6):  660-663.  doi:10.7525/j.issn.1673-5102.2010.06.006
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    Based on the lectotype of Swietenia mahagoni and the holotype of S.macrophylla, the status of those species as cited in Chinese taxonomic literature, such as Acta Phytotaxonomica Sinica, Flora of Guangzhou, Iconographia Cormophytorum Sinicorum, Flora Yunnanica, Flora of Guangdong, Flora Reipublicae Popularis Sinicae and Higher Plants of China, the name S.mahagoni has been misapplied in above-mentioned Chinese taxonomic literature and should be Swietenia macrophylla King. Swietenia mahagoni (L.) Jacq. has leaflets less than 10 cm long and oval fruits less than 15 cm long; S.macrophylla King has always a quantity of leaflets more than 10 cm long and club-shaped fruits 12-22 cm long.
    A Revise of Stamen Number to an Endangered Plant:Rhododendron shanii Fang
    ZHAO Kai;YANG Wen-Hu;CHEN Wen-Long;SHAO Jian-Wen*
    2010, 30(6):  664-667.  doi:10.7525/j.issn.1673-5102.2010.06.007
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    We corrected the stamen number(a key identification feature) of Rhododendron shanii from 10 to 12-15 through surveying the stamen number of R.shanii from five populations(containing typical locality), investigating plenty of specimens and interviewing the man who collected the type specimens. We also compared the morphology of R.shanii with R.fortunei which were planted in the same location, the result showed that there were many differences between the morphology of these two species, proving that R.shanii is an independent ‘good species’.
    Direct Organogenesis and Plantlet Regeneration from Mature Zygotic Embryos of Chinese Pine(Pinus tabulaeformis Carr.)
    KONG Dong-Mei;WAN Ting
    2010, 30(6):  668-673.  doi:10.7525/j.issn.1673-5102.2010.06.008
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    Mature zygotic embryos of Chinese pine were cultured as initial explants to investigate the process of direct organogenesis. Adventitious buds were initiated on DCR and MS medium supplemented with 1~5 mg·L-1 benzyladenine (BA) with or without 1~10 mg·L-1 2,4-dichlorophenoxyacetic acid (2,4-D). The highest induction frequency of adventitious buds (90%) was obtained on DCR medium containing 1 mg·L-1 BA. Subsequent transfer of buds to plant growth regulator (PGR)-free medium in time was advantageous for elongation of adventitious buds. 1/2DCR medium with 1 mg·L-1 indole-3-butyric acid (IBA) was suitable for adventitious root initiation, on which average rooting frequency was 47.1%. Plantlets were successfully acclimatized in vermiculite and transferred to greenhouse, where they had a survival rate of 86.4% and no morphological abnormalities.
    Effects of La(NO3)3 on Plant Growth and Leaf Photosynthetic Physiology of Ryegrass(Lolium pernne L.) Seedlings under Alkaline Stress
    LIU Jian-Xin;WANG Xin;HU Hao-Bin;WANG Rui-Juan;LI Dong-Bo
    2010, 30(6):  674-679.  doi:10.7525/j.issn.1673-5102.2010.06.009
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    Effects of different concentrations of La(NO3)3 on growth of ryegrass seedlings and regulatory effect on photosynthetic physiological response in ryegrass leaves under 150 mmol·L-1 NaHCO3 stress were investigated. The ryegrass leaves were sprayed by low concentration(0.05 mmol·L-1) of La(NO3)3 solution for 3 days before NaHCO3 stress. The results showed that La(NO3)3 pretreatment significantly alleviated the decreases of the fresh and dry weights of ryegrass seedlings, the leaf chlorophyll and carotene contents, the activities of Hill reaction, electron transport, photophosphorylation, Mg2+ATPase and RuBPCase in chloroplast, and photosynthetic rate by alkaline stress, but no effect on glycolic acid oxidase activity was observed. However, pretreatment of La(NO3)3 with high concentration(0.5 mmol·L-1) had no alleviation effect on alkaline stress, instead, aggravated the injury by alkaline stress on ryegrass seedlings. These results suggested that the pretreatment of feasible concentration of La(NO3)3 could alleviate the inhibitory effect on the growth of ryegrass seedlings by alkaline stress via improving leaf photosynthetic function.
    Variations in Photosynthetic Capacity and Growth Characters for 2-Needled Pine from Diverse Geographic Populations
    MA Li-Xiang;WANG Xiu-Wei;MAO Zi-Jun*;LIU Lin-Xin;SUN Tao
    2010, 30(6):  680-684.  doi:10.7525/j.issn.1673-5102.2010.06.010
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    To understand responses and adaptation of 2-needled pine photosynthesis to environmental changes, we conducted a provenance experiment on Pinus densiflora Sieb. et Zucc(5 provenances),Pinus sylvestris var. sylvestriformis(1 provenance),and Pinus sylvestris var. mongolica(3 provenances) respectively. Seeds, collected from 9 provenances with significantly different geographical and climatic conditions, were planted and grew up in greenhouse of Northeast Forestry University. Measurement of the seedlings was conducted after two years. Significant diffrence of lightsaturated net photosynthetic rate(Pmax)(p=0.18), dark respiration rate(Rd)(p=0.15) and apparent quantum yield(AQY)(p=0.18) was not be found among the three species, but was found among the 9 provenances. AQY from Xingkaihu showed the highest (0.084 5±0.002 4 mol CO2·mol-1 photons), which was higher 13.10%~159.23% than that of other provenances. Rd was high from Heihe and Xingkaihu provnences(1.62±0.18 μmol CO2·m-2·s-1, 1.52±0.30 μmol CO2·m-2·s-1 respectively) and lower from Antu and Dongning provenances(0.40±0.01 μmol CO2·m-2·s-1, 0.34±0.03 μmol CO2·m-2·s-1 respectively). There are significant diffrence of Pmax among the 9 provenances(p=0.02) averaging 18.36±1.81 μmol CO2·m-2·s-1 from Xingkaihu, Dongning, Korea, Jidong, Erdaobaihe and Honghuaerji, which higher than that of from Antu, Mohe and Heihe averaging 12.57±0.86 μmol CO2·m-2·s-1. Significant difference was found among the populations in height of seedlings and base stem diameter. Among thises populations, the height of seedlings is highest for Korean population, and lowest for Heihe population. The base stem diameter of seedlings is highest for Xingkaihu population and lowest for Antu population. The maximum of these two characters is 3 times higher than the minimum. The significant variations in the photosynthetic capacity and some related factors from the nine 2-needled pine provenances suggest an evident of ecophysiological adaptation of photosynthesis to their original environmental conditions.
    Effects of Cd Stress on the Seed Germination,Seedling Growth and Activities of Antioxidant Enzymes in Petunia hybrida
    FAN Qing;LÜXiu-Jun;YANG Liu;YANG Ying-Li*
    2010, 30(6):  685-691.  doi:10.7525/j.issn.1673-5102.2010.06.011
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    Effects of different cadmium (Cd) concentrations (0, 1, 2 and 4 mmol·L-1) on the seed germination, seedling growth and physiological property of Petunia hybrida were studied. In comparison with the control, seed germination and seeding growth exhibited a significant decrease at low level of Cd2+ concentration(1 mmol·L-1), and these factors were all completely restrained at high concentration(4 mmol·L-1). Compared to the control, the activities of peroxidase (POD) and catalase (CAT) were enhanced in P.hybrida seedings exposed to low Cd concentrations but inhibited at higher concentrations; the activities of ascorbate peroxidase (APX) and superoxide dismutase (SOD) could be increased in response to different Cd concentrations, no significant change in the malonaldehyde (MDA) content was observed. Ethylene-aminetetraacetic acid (EDTA) could stimulate the activities of POD and CAT in P.hybrida seedlings exposed to 4 mmol·L-1 Cd treatment, and MDA content was also enhanced when seedlings were treated with 4 mmol·L-1 Cd in the presence of 6 mmol·L-1 EDTA. In conclusion, these results indicate that Cd stress significantly inhibit P.hybrida seed germination and seedling growth, but stimulate the activities of POD, CAT and APX in the leaves of seedlings; the application of EDTA further enhance the activities of POD and APX in Cd-treated P.hybrida seedlings. Therefore, higher activities of antioxidative enzymes may protect P.hybrida seedlings against Cd-induced oxidative change.
    Responses of Three Native Shrubs to Drought Stress in Urumqi
    ZHAO Fa;ZHAO Xiao-Ying*;JIN Wan-Gui;DONG Zheng-Wu
    2010, 30(6):  692-696.  doi:10.7525/j.issn.1673-5102.2010.06.012
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    Salsola arbuculaConvolvulos tragacanthoides and Atraphaxis spinosa are important components of the vegetation in Urumqi. They play an important role in maintaining ecosystem stability in the area. The responses to drought stress on growth and physiological characteristics of these shrubs were studied in this paper. The results showed that the height, basal diameter and the biomass of these shrubs were differently decreased at the three levels of water stress. The distribution of biomass was also affected by drought stress. The growth of C.tragacanthoides hardly decreased with the water stress. With the drought stress, the contents of malondialdehyde (MDA), proline and soluble sugar increased at different levels. The content of chlorophyll decreased. In conclusion, S.arbucula and A.spinosa grew well under the mild water stress. C.tragacanthoides grew better under moderate and severe water stress.
    Variation of Endogenous Hormone Contents in Bulbs of Two Lycoris During Growth and Development
    JIN Ya-Qin;HUANG Xue-Fang;LI Dong-Lin;XIANG Qi-Bai
    2010, 30(6):  697-702.  doi:10.7525/j.issn.1673-5102.2010.06.013
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    Variations of endogenous hormone contents in bulbs of both Lycoris radiata and Lycoris chinensis in different growth and development stages were studied with enzyme linked immunosorbent assay(ELSA),the relationship between endogenous hormone and growth and developmen of two Lycoris plants were analyzed. The results showed the IAA content of two plants was low, but the contents of iPAs,ABA and GAs were high during the fruit bud differentiation stage. With the coming of florescence, the IAA content increased, while the contents of iPAs,ZRs,ABA and GAs fell to extremum. During florescence the contents of ABA,GAs and ZRs increased, this suggested the increasing of ABA and GAs contents played a stimulative role in the flowering of two plants, but the role in the growth of their leaves was not obvious. Relatively high ZRs/IAA, GAs/IAA and ABA/GAs ratios were beneficial for their floweral differentiation, while moderately decreasing of three ratios did the same for leaves growth.
    Effects of Increased Endogenous Ethylene on Plant Salt Tolerance in Arabidopsis Seedlings under Saline Condition
    LIN Ying-Chao;YANG Lei;ZHAO Xiao-Ju;TANG Zhong-Hua*
    2010, 30(6):  703-707.  doi:10.7525/j.issn.1673-5102.2010.06.014
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    The effects of endogenous ethylene on plant salt tolerance in Arabidopsis seedlings in exposure to salinity were investigated in the present work. The results showed that 100 mmol·L-1 NaCl significantly decreased seedling root elongation and root fresh weight. Application of exogenous ethephon obviously alleviated salt-induced inhibition of root length and biomass accumulation. Comparatively, exogenous ACC had a positive effect merely on alleviating root weight inhibition caused by salt stress. The root ROS level in salt-stressed seedlings was also increased drastically, and both ethephon and ACC could keep the root ROS level at a low level as control under NaCl stress conditions. It was proposed that improvement of Arabidopsis salt tolerance induced by ethylene is tightly related to root redox state.
    Physical and Chemical Properties of 2 Species in Bamboos from the Different Regions in Sichuan Province
    HU Shang-Lian;JIANG Yao;CHEN Qi-Bing;CAO Ying;SUN Xia;LU Xue-Qin
    2010, 30(6):  708-712.  doi:10.7525/j.issn.1673-5102.2010.06.015
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    The changes of cellulose content and lignin content and fiber morphology were studied for Neosinocalamus affinis and Dendrocalamus farinosus with the typical thick-growing characteristics from the different regions in Sichuan province. The results showed that the cellulose content and lignin content were significantly different between the different species in the same region. The cellulose and lignin content, the length and width of fiber and their aspect ratio within the same specie of bamboo in the different regions were related to the different regions and the age of bamboos. Taking account of good fiber material of bamboo for paper making, N.affinis(cellulose content is about 45%, lignin content is below 28%)and D.farinosus(cellulose content is over 50%, lignin content is below 20%)from Zhuhai region are suitable for pulp making.
    Construction of cDNA Library in Grimmia pilifera Under Drought Stress
    SONG Xiao-Hong;SHA Wei*;LIN Lin;WANG Gui-Yun;ZHANG Yan-Fu;JIN Zhong-Min
    2010, 30(6):  713-717.  doi:10.7525/j.issn.1673-5102.2010.06.016
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    Drought is the major environmental factor that influences the growth of plant and accounts for significant reduction in the yields of crops. An effective approach to solve this problem is to produce drought-tolerant crops. Therefore, comparative functional genomics studies are required to identify key genes responsible for dehydration and drought stress tolerance as well as candidate genes for genetic engineering of drought stress tolerance in crop plants. Grimmia pilifera, as a common drought tolerant moss, grows in habitats of rocky outcrops, having a high ability in resisting drought stress. G.pilifera may serve as a rich genetic resource for the identification of novel genes associated with environmental stress and dehydration tolerance. To identify genes involved in drought tolerance for genetic engineering of biotic and abiotic stress tolerance in crop plants, a cDNA library was constructed with G.pilifera under drought stress by using CreaterTM SMARTTM cDNA Library Construction Kit. The total RNA was extracted by improved SDS method. The library had a titer of 2.8×105 pfu·mL-1, and the recombination rate was 91.7%. PCR amplification revealed that the insert cDNA fragments ranged mostly from 500 to 2 000 bp, with an average length of 800 bp. A total of 1 045 cDNA clones were randomly sequenced and analyzed. High-quality ESTs were obtained for 996 clones representing 875 unique sequences. The constructed cDNA library provided an essential for screening and cloning of new drought resistance associated genes of G.pilifera.
    Analysis and Comparison of Flavonoids in Three Bougainvilleas by Micellar Electrokinetic Chromatography
    XU Su-Xia;LIN Chun-Song;HUANG Qing-Yun;LI Li;ZENG Bi-Yu
    2010, 30(6):  718-724.  doi:10.7525/j.issn.1673-5102.2010.06.017
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    The exclusion distribution of betalain and flavonoid-derived anthocyanins and existing of flavonoids in betalain-plants led to our research. Quercetin, kaempferol, myricetin, apigenin, and isorhamnetin were simultaneously detected in three white varieties of Bougainvillea(Bougainvillea sp., Bougainvillea glabra ‘Alba’, and Bougainvillea ‘White Stripe’) for the first time, using micellar electrokinetic chromatography (MEKC). Among the five flavonoids, the three flavonols were detected, and one flavone and one flavonol were not. The total content of the three flavonoids were 1 749 μg·g-1 in B.glabra ‘Alba’, 1 241 μg·g-1 in B. ‘White Stripe’ and 1 312 μg·g-1 in B. sp. In the detected flavonoids, the content of quercetin was the highest and ranged from 852-1 114 μg·g-1, isorhamnetin varied from 161-628 μg·g-1 in three species. Kaempferol was detected at trace amounts. This work is valuable to identify and quantify the naturally occurring flavonoids in the edible bracts of three white varieties of Bougainvillea. The relationship between betalain and flavonoids in bougainvilleas and possible functions were discussed.
    Renovation of ROP GTPase in Young Seedlings of Wheats by He-Ne Laser under Enhanced UV-B Radiation
    AN Xu-Liang;HAN Rong*
    2010, 30(6):  725-730.  doi:10.7525/j.issn.1673-5102.2010.06.018
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    Using He-Ne laser irradiation to repair the ROP GTPase damage by the enhanced UV-B radiation in wheat seedlings has been studied. We extracted small G protein, and then determined its content by SDS-PAGE electrophoresis. At the same time, we tested the FITC fluorescence intensity of wheat microfilament marker by using a laser scanning confocal microscope. The results showed that, the enhanced UV-B radiation reduced the Rop protein content in wheat seedlings, and we also found that it changed the shape of protoplast by LCSM scanning.The intensity of the fluorescence which marks damaged cytoskeletal by the enhanced UV-B radiation was weaken. Moreover, in the whole period of UV-B treatment, the fluorescence degree was lower than the control group (CK). Then, after treating with the He-Ne laser, the content, the shape and the intensity were all increased, but still lower than the control guoup. From the above descriptions, we can say that the UV-B radiation could decrease the Rop GTPase content of wheat seedlings and damage microfilament cytoskeleton. However, a fixed dose of laser played a role in repairing the low protein content and the cell cytoskeleton damaged by the UV-B radiation. By the way, the Rop proteins participate in the reorganization of the actin cytoskeleton.
    Analysis of Interspecific Relationships among the Dominant Populations of Platycladus orientalis Communities in Yanshan Nature Reserve in Shanxi
    GAO Ping;ZHANG Gui-Ping;WU Qiong;LIAN Jun-Qiang;ZHANG Feng;*
    2010, 30(6):  731-736.  doi:10.7525/j.issn.1673-5102.2010.06.019
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    Based on the datasets from the field, 2×2 contingency table, the interspecific relationships among 24 dominant populations of Platycladus orientalis communities in Yanshan nature Reserve in Shanxi were studied by using variance ratio(VR) test, Fisher’s exact test, Jaccard’s association index and Spearman rank correlation coefficient. The results indicated that the overall association of the community was not significant (p>0.05) and tended to no correlation among all species, and it meant that the species of the community were mostly independently distributed. The combination of Fisher exact test and Jaccard’s association index in the study obtained a better result. The results of Jaccard’s association index showed that the association intensity of species pairs between the main dominant populations of each layer was more significant. The results also showed that the dominant populations of P.orientalis communities could be divided into three biological groups: Pinus bungeana group, P.orientalis group and Artemisia sacrorum group.
    Effect of Grazing Interference on the Architecture of Sibiraea angustata in the Eastern Qinghai Tibetan Plateau
    YIN Liang;LI Shao-Rong;ZHOU Min;WANG Qian*;WU Yan;LUO Peng
    2010, 30(6):  737-741.  doi:10.7525/j.issn.1673-5102.2010.06.020
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    Study on the structure of Sibiraea angustata shrubs at Kaka valley, Songpan county in Northwestren Sichuan (eastern edge of Qinghai-Tibetan Plateau) showed that its architecture was significantly influenced by grazing interference. Total biomass, total leaf area and sapwood area of individual ramets decreased significantly under heavy grazing intensity. Single leaf length, width and area enlarged significantly under moderate and heavy grazing intensities. Though maximum individual height was reached under moderate grazing intensity, the extent of aggregation of leaf and shoot biomass at top part decreased. The variation of architecture did not change the leaf area supported by unit sapwood area. Phenotypical plasticity under grazing is still controlled by Huber value to maintain the balance between leaf transpiration and hydraulic conductivity of stem.
    Relationships Between the Growth and Taxol Content of Taxus chinensis var. mairei and Environment Factors
    YANG Feng-Jian;PANG Hai-He;ZU Yuan-Gang*;ZHANG Xue-Ke;GAO Yin-Xiang
    2010, 30(6):  742-746.  doi:10.7525/j.issn.1673-5102.2010.06.021
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    The growth characteristics and the taxol content in the leaves and branches of Taxus chinensis var. mairei and the environment factors were studied. The correlativity between the growth characteristics and the taxol content in the leaves and branches of T.chinensis var. mairei and the environment factors showed that there was a extremely significant positive correlation between the growth and the air and soil humidity, and there was a significant positive correlation with the air and soil temperature, soil N content, soil K content, soil organic matter and soil tensity and a negative correlation with the net radiation and soil P content, but the correlation was not significant, which indicated that the high temperature and humidity and shade was favored to the growth of T.chinensis var.mairei, which needed more soil P content; There was a negative correlation between the taxol content and the air and soil humidity, the soil N, P, K content and a positive correlation with air and soil temperature, net radiation soil organic matter and soil tensity, which indicated that high temperature, high light tensity and drought were favored to the increase of taxol content, which needed more soil N, P, K content, especially the soil N and K content. There was a negative correlation between the growth and the taxol content, but the correlation was not significant, which indicated that taxol may be defense substance, which made the T.chinensis var. mairei adapt to the adversity, such as high temperature, drought, high light tensity and so on.
    Stand Biomass Prediction Model for Larix olgensis Plantation
    JIN Zhong-Yue;JIA Wei-Wei*;LIU Wei
    2010, 30(6):  747-752.  doi:10.7525/j.issn.1673-5102.2010.06.022
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    Based on the biomass data from stem and branch analysis of 95 trees in 19 sample plots, with different ages and densities of larch(Larix olgensis) plantation as study object, the relationship between different factors (diameter at breast height, tree height, crown, etc.) and individual tree components (stem, branch, leaf) biomass was studied, and the regression models for various parts of biomass of individual larch were established using statistical analysis software. The regression models were used to estimate the total biomass of larch plantation stand. Variation of stand biomass in different ages and stand densities was analyzed. It showed that stand biomass increases with age, the proportion of stem biomass is the largest, which also increases with age continuously, but the proportion of biomass of branches and leaves is relatively small. Stand biomass increases with stand density. Finally, stand biomass model for larch is established. The results provided basic information on larch plantation for research and scientific basis for its reasonable management, facilitated the understanding of the productivity of larch plantation.
    Seed Dormant Characteristics of Polygonatum sibiricum Red.
    ZHANG Yue-Jin;ZHANG Yu-Cui;LI Yong-Gang;LIU Feng;GUO Hong-Bo*
    2010, 30(6):  753-757.  doi:10.7525/j.issn.1673-5102.2010.06.023
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    The seed dormant characteristics of Polygonatum sibiricum Red. were studied in the aspects of seed morphology, anatomical structure, bioassay of some inhibitors in fruits and seeds, and the changes of endogenous hormones ABA content during seed ripening as well. The results showed that the seed dormancy was comprehensive. First, physiological past-ripening was the main reason for seed dormancy after autumn harvesting; Second, the symplast transportation was limited because of endosperm cell with small size, thick cytoplasm, close arrangement with tiny intercellular space; Third, germination inhibitors existed in fruits and seeds; Finally, the increase of ABA content at maturity also caused the seed dormancy.
    Inhibitory Effect of Algae Growth and HPLC Analysis of Allelochemicals from Stephania tetrandra
    CHAI Min-Wei;SHI Fu-Chen*;MA Yan
    2010, 30(6):  758-762.  doi:10.7525/j.issn.1673-5102.2010.06.024
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    Aqueous extracts of 12 kinds of medicinal plants were obtained by soak extraction, and their inhibitory effects on algae growth were studied. The results showed that: when the relative concentration was 1 g·L-1, aqueous extracts of Stephania tetrandra had the strongest inhibitory effect on the growth of Chlorella pyrenoidosa, and the maximum specific growth rate was -0.28 d-1. The methanol extract of S.tetrandra had significant and long-lasting inhibitory effect on the growth of C.pyrenoidosa and the lowest effective concentration in inhibiting algae was 30 mg·L-1.The identification results of HPLC showed that: the main constituents in the extracts were alkaloids such as fangchinoline and tetrandrine, etc.S.tetrandra had great prospect of application in controlling water bloom.
    Research Progress in the Penetrability and Drought resistance of Plant Cuticular
    CHAI Ling-Yan;DONG Yi-Fan;LI Shi-Wei;SHI Tuan-Sheng*
    2010, 30(6):  763-768.  doi:10.7525/j.issn.1673-5102.2010.06.025
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    The plant cuticular covers aerial plant organs, forms a layer between the plant and the environment, and plays an important role in protecting aerial organs from damage caused by biotic and abiotic stresses. The structure, composition, biosynthesis of plant cuticular and the penetrability of cuticular as well as the investigation concerning the relationship between the cuticular and the plant drought-resistance are reviewed .We also discussed the problems existing in the cuticular research.