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Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (2): 191-196.doi: 10.7525/j.issn.1673-5102.2013.02.012

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Transformation and Expression of 1Dx5 without Selectable Markers in Common Wheat Xindong No.26

WANG Yong;QIN Jian-Bing;FAN Ling;ZHU Chang-Qing*   

  1. 1.Research Room of Molecular Biology and Informatics,Xinjiang Normal University,Urumqi　830053；2.Xinjiang Academy of Agricultural Sciences,Urumqi　830091
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-03-20 Published:2013-03-20
  • Contact: ZHU Chang-Qing
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Abstract: To improve the bread-making quality of wheat, the Xinjiang winter common wheat Xindong No.26 were transformed with high-molecular-weight glutenin subunits (HWM-GS) gene 1Dx5 that without selectable markers by particle bombardment. Transformed plants were screened by PCR, three transgenic plants were detected among 1 000 transformed plants, yielding a transformation efficiency of 0.3%. The compositions of HWM-GS were analyzed by SDS-. The HMW-GS gene 1Dx5 was expressed in some seeds of transgenic plants. The high molecular weight glutenin subunit gene 1Dx5 was transformed into wheat XinDong No.26 and was expressed in some transgenic progenies. Our studies lay the foundations for the improvement of bread-making quality of wheat.

Key words: wheat, selectable markers, 1Dx5, microprojectile

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