Germination Obstacle Factors of Berberis Seeds of Different Natural Populations

doi:10.7525/j.issn.1673-5102.2018.06.013

Abstract

Abstract: The Black-fruit Berberis seeds of Huocheng population, Xinyuan population, Tekesi population and the Red-fruit Berberis of Tekesi population were used as experimental materials to study their biological characteristics, water absorption characteristics and the effects of seed coat removal, low temperature stratification and different concentrations of GA₃ treatment on the dormancy and germination characteristics.The results showed that:(1) The water absorption rate of four groups of wild Berberis seeds showed a trend of increasing gradually.There was no significant difference in water absorption between Black-fruit Berberis seeds and Red-fruit Berberis seeds, and the water permeability of their seed coat was similar; (2) The seed coats of Black-fruit Berberis seeds of Huocheng population, Xinyuan population and Tekesi population had a strong inhibitory effect on germination.However the seed coat of Red-fruit Berberis seeds in Tekesi population had no obvious inhibition on germination; (3) Low temperature stratification had a great effect on the germination of wild Berberis seeds in 4 groups, and the germination rate of Berberis seeds increased gradually with the increase of stratification time.The dormancy of Black-fruit Berberis seeds was basically relieved after 90 days of stratification;the dormancy of Red-fruit Berberis seeds was basically relieved after 30 days of stratification; (4) GA₃ solution with 200 mg·L^-1 concentration could significantly increase the germination rate of the four groups Berberis seeds, and too high or too low concentration could inhibit the germination of Berberis seeds. After 60 days of layering,the germination rates of Black-fruit Berberis seeds without seed coat of Huocheng population, Xinyuan population and Tekesi population were 85.00%, 83.33%, and 86.67% respectively after treated with GA₃ solution of 200 mg·L^-1 for 2 hours;When the Red-fruit Berberis seeds were layered for 15 days,the germination rates of Red-fruit Berberis seeds without seed coat of Tekesi population were 86.67% after treated with GA₃ solution of 200 mg·L^-1 for 2 hours.The results will provide technical support and scientific basis for the introduction and breeding of wild Berberis.

Key words: Berberis, natural populations, seeds germination, low temperature stratification, GA₃

CLC Number:

Q949.746.8

LI Lin-Yu, FANG Zi-Yan, Maola Aikebaier, ZHOU Long, LU Biao. Germination Obstacle Factors of Berberis Seeds of Different Natural Populations[J]. Bulletin of Botanical Research, 2018, 38(6): 894-901.

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