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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (2): 260-267.doi: 10.7525/j.issn.1673-5102.2018.02.014

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Construction and Functional Verification of Plant Expression Vector of Tamarix ThDUF106 Gene

GE Xiao-Lan, LIU Cai-Xia, ZHANG Xin-Xin, LI Ying, QU Guan-Zheng   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2017-09-27 Online:2018-03-15 Published:2018-03-23
  • Supported by:
    State key laboratory of forest genetics and breeding(Northeast Forestry University) Innovation project(2015A01);The National Natural Science Fund(31370661)

Abstract: ThDUF106 gene were involved in the stress tolerance of Tamarix hispida, and the expression of ThDUF106 was up-regulated in T.hispida under salt-alkali stress. In order to study the function of ThDUF106 gene in T.hispida, the temporal and spatial expression of these gene was detected by qRT-PCR in the leaves and roots of three-month T.hispida under abiotic stress such as salinity, drought and heavy metals. The gene encoding this gene was also analyzed, and the gene was constructed and transformed into tobacco by the plant expression vector. Transgenic tobacco lines and wild-type one-month seedlings were treated, and physiological and biochemical indexes were compared and analyzed. The antioxidant status of transgenic and wild type tobacco was basically the same under various duress, indicating that the gene was not expressed in tobacco and could not improve the antioxidation of tobacco.

Key words: Tamarix hispida, ThDUF106, genetic transformation, tobacco, abiotic stress

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