Abstract: With Catharanthus roseus, we used perlite as the medium quality nutritional way to study the effects of copper stresson theresponses of C.roseus seedling growth, accumulation, gene expression and characteristics of alkaloid synthesis. The dry weight of root and leaf of C.roseus under exogenous Cu stress levels was increased under low concentration,decreased under high concentration,and reached peak at 300 μmol·L^-1, with significant difference compared with the control(P<0.05). The root tolerance index of seedlings was increased firstly, then decreased, and reached the peak at 200 μmol·L^-1 of Cu. Cu into C.roseus seedlings was mostly accumulated in roots. But under the stress of exogenous Cu, C.roseus blade parameters, such as the accumulation of dry matter, was improved significantly, by presenting a regular change the concentration was increased, and reached the peak at 300 μmol·L^-1 Cu, even reaching as much as 2.7 times thanthe CK. The C.roseus hyperaccumulation plants can be usedas further reference basisfor Cu accumulation.

Key words: Catharanthus roseus, copper stress, vinblastine, hyperaccumulation plants of Cu