Key Technology of Micropropagation of Osmanthus fragrans var. thunbergii

doi:10.7525/j.issn.1673-5102.2019.03.005

Abstract

Abstract: We studied the effects of different rooting media, pre-treatment of seedling cultivation, transplanting matrix and leaf surface fertilization on the seedling cultivation of micro-propagation of Osmanthus fragrans to provide technical support for the rapid propagation of strong seedlings of target varieties, and the key technology of rapid propagation of O.fragrans. The optimal combination of rooting rate and rooting number of micro-propagated O.fragrans seedlings was A₃B₂C₃, the rooting rate and average rooting number of B₅ basic medium+2 mg·L^-1 NAA+0.10 mg·L^-1 6-BA were 91.11% and 3.83 pieces/plant, respectively. A₃B₂C₃ is the optimal combination for seedling refining, that is, when the nursery soil:perlite:humus=2:3:7 was used as the seedling refining matrix, the survival rate and growth rate of micropropagation seedlings of O.fragrans reached 82.2% and 42.2%, respectively.

Key words: Osmanthus fragrans, micropropagatio, hardening, transplantings

CLC Number:

S685.13

YE Tian-Yi, ZHOU Xin, HUANG Yan, WANG Xiao-Hong. Key Technology of Micropropagation of Osmanthus fragrans var. thunbergii[J]. Bulletin of Botanical Research, 2019, 39(3): 358-364.

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