Abstract: Changes in chlorophyll content, composition of chloroplast protein and root meristems protein in rye and maize treated by AmiprophoseMethyl (APM) and Atrazine were studied. Results revealed that for the concentrations of 0.1 mg·L^-1 and 1 mg·L^-1 Atrazine, chlorophyll contents of rye decreased from 1.72±0.034 mg·g-1 FW (control) to 1.62±0.05 mg·g^-1 FW and 1.25±0.015 mg·g^-1 FW, respectively. Both kinds of herbicides could induce the protein composition changes of root meristems in rye and maize, when rye and maize were treated with 0.1 mg·L^-1 Atrazine, 4 protein spots, spot 7, spot 18~20 were induced and 12 protein spots, spot 6, spot 8~17 and spot 21 were lost in root meristems of rye, 4 protein spots, spot 5, spot 14~16 were induced and 4 protein spots, spot 17~20 were lost in root meristems of maize. 2 protein spots, spot 19~20 were induced and 13 protein spots, spot 1~13 were lost in chloroplast of rye, however, Atrazine could not cause the contents of chlorophyll and chloroplast protein composition changes in maize. For the concentration of 4 mg·L^-1 APM, 1 protein spot, spot 1 was induced and 4 protein spots, spot 2~5 were lost in root meristems of rye, and 15 protein spots, spot 4~5, spot 7~16, spot 21~23 were induced and 4 protein spots, spot 1~3, spot 6 were lost in root meristems of maize. No effect of APM on the contents of chlorophyll and protein compositions of chloroplast was observed in two crops.

Key words: herbicide, chloroplast protein, 2D-PAGE, rye, maize