Resistance Capability of Aquilaria sinensis under Combinations of Inorganic Salts,Hormones and Fungus Induction

doi:10.7525/j.issn.1673-5102.2019.04.004

Abstract

Abstract: We explored the effect of different combinations of inorganic salts hormones and fungus induction on resistance capability of Aquilaria sinensis and the relationship between resistance and discoloration length of wood core in early stage of agarwood. Two induction experiments bydifferent combination of 3 kinds of inorganic salts, 3 hormones and 3 fungi were carried out for ten-years-old trees of A. sinensis by uniform experimental design. The results showed that:(1)POD and SOD activity and MDA content firstly increased and then decreased; CAT activity reached the highest value at 1 day after induction, and then decreased; (2)The resistance capability under combinations of inorganic salts and fungus induction was greater than that of hormones and fungus. The capability in treatment 4(1.0% CaCl₂+0.5% FeSO₄+2.0% NaCl+Trichoderma atroviride:Fusarium solani:Lasiodiplodia theobromae(1:1:1)) from combinations of inorganic salts and fungus and treatment 2(0.01% Methyl Jasmonate+0.1% Ethephon+0.2% Sodium salicylate+Trichoderma atroviride:Fusarium solani:Lasiodiplodia theobromae(1:1:1)) from combinations of hormones and fungus was higher in the experiments respectively; (3)There was a significant positive correlation between the resistance capability of A. sinensis and the length of wood core discoloration; (4)Theoretically, 0.93% CaCl₂+0.53% FeSO₄+2.5% NaCl+Trichoderma atroviride:Fusarium solani:Lasiodiplodia theobromae(1:1:1) and 0.005% Methyl Jasmonate+0.006% Ethephon+0.2% Sodium salicylate+Trichoderma atroviride:Fusarium solani:Lasiodiplodia theobromae(1:1:1) was combination with the highest resistance ability respectively.

Key words: Aquilaria sinensis, inorganic salts, hormones, fungus, artificial induction, resistance capability

CLC Number:

Q949.761.1

SONG Xiao-Chen, HUANG Gui-Hua, WANG Xi-Yang, SONG Jie, ZHANG Qing-Qing, LIANG Kun-Nan, ZHOU Zai-Zhi. Resistance Capability of Aquilaria sinensis under Combinations of Inorganic Salts,Hormones and Fungus Induction[J]. Bulletin of Botanical Research, 2019, 39(4): 505-513.

References

1. 中国科学院中国植物志编辑委员会. 中国植物志[M]. 北京:科学出版社,1999:290-291.
Editorial Board of Chinese Botany Chinese Academy of Sciences. Flora of China[M]. Beijing:Science Press,1999:290-291.
2. 张争,杨云,魏建和,等. 白木香结香机制研究进展及其防御反应诱导结香假说[J]. 中草药,2010,41(1):156-159.
Zhang Z,Yang Y,Wei J H,et al. Advances in studies on mechanism of agarwood formation in Aquilaria sinensis and its hypothesis of agarwood formation induced by defense response[J]. Chinese Traditional and Herbal Drugs,2010,41(1):156-159.
3. Wojtaszek P. Oxidative burst:an early plant response to pathogen infection[J]. Biochemical Journal,1997,322(3):681-692.
4. 辛松林,秦文,孙传红,等. 腐皮镰孢霉菌侵染及保鲜剂处理对秋葵相关抗性酶的影响[J]. 江苏农业学报,2018,34(5):1161-1168.
Xin S L,Qin W,Sun C H,et al. Effects of Fusarium solani infection and preservative treatment on resistance associated enzyme from okra[J]. Jiangsu Journal of Agricultural Science,2018,34(5):1161-1168.
5. Deinlein U,Stephan A B,Horie T,et al. Plant salt-tolerance mechanisms[J]. Trends in Plant Science,2014,19(6):371-379.
6. 刘威,袁晓婷,张艳艳,等. 胭脂红景天引种至西藏日喀则其渗透调节物质及保护酶活性的变化[J]. 植物研究,2013,33(6):697-700.
Liu W,Yuan X T,Zhang Y Y,et al. Changes of osmotic adjustment substances and activities of protective enzymes in Sedum spurium ‘Coccineum’ introduced to Xigaze,Tibet[J]. Bulletin of Botanical Research,2013,33(6):697-700.
7. 张兴丽. 伤害诱导的白木香防御反应与沉香形成的关系研究[D]. 北京:北京林业大学,2013.
Zhang X L. Studies on relationships between wound-induced defense response and agarwood formation in Aquilaria sinensis[D]. Beijing:Beijing Forestry University,2013.
8. 王东光. 白木香结香促进技术研究[D]. 北京:中国林业科学研究院,2016.
Wang D G. Study on agarwood-induced technique of Aquilaria sinensis[D]. Beijing:Chinese Academy of Forestry,2016.
9. 王东光,张宁南,杨曾奖,等. 人工诱导白木香树体抗逆能力的研究[J]. 华南农业大学学报,2016,37(6):70-76.
Wang D G,Zhang N N,Yang Z J,et al. Study on resistance ability of Aquilaria sinensis trees under artificial induction[J]. Journal of South China Agricultural University,2016,37(6):70-76.
10. Hillis W E. Chemical aspects of heartwood formation[J]. Wood Science and Technology,1968,2(4):241-259.
11. Onuorah E O. Relative efficacy of heartwood extracts and proprietory wood preservatives as wood protectants[J]. Journal of Forestry Research,2002,13(3):183-190.
12. Mohamed R,Jong P L,Irdayu I N. Succession patterns of fungi associated to wound-induced agarwood in wild Aquilaria malaccensis revealed from quantitative PCR assay[J]. World Journal of Microbiology and Biotechnology,2014,30(9):2427-2436.
13. 王小菲,高文强,刘建锋,等. 植物防御策略及其环境驱动机制[J]. 生态学杂志,2015,34(12):3542-3552.
Wang X F,Gao W Q,Liu J F,et al. Plant defensive strategies and environment-driven mechanisms[J]. Chinese Journal of Ecology,2015,34(12):3542-3552.
14. Giannopolitis C N,Ries S K. Superoxide dismutase:Ⅰ. occurrence in higher plants[J]. Plant Physiology,1977,59(2):309-314.
15. 张志良,瞿伟菁,李小方. 植物生理学实验指导:4版[M]. 北京:高等教育出版社,2009.
Zhang Z L,Qu W J,Li X F. Plant physiology experimental guidance:4th ed[M]. Beijing:Higher Education Press,2009.
16. Dhindsa R S,Pamela Plumb-Dhindsa P,Thorpe T A. Leaf senescence:correlated with increased levels of membrane permeability and lipid peroxidation,and decreased levels of superoxide dismutase and catalase[J]. Journal of Experimental Botany,1981,32(126):93-101.
17. 王谧,王芳,王舰. 应用隶属函数法对马铃薯进行抗旱性综合评价[J]. 云南农业大学学报,2014,29(4):476-481.
Wang M,Wang F,Wang J. Evaluation of potato drought resistance by subordinate function[J]. Journal of Yunnan Agricultural University,2014,29(4):476-481.
18. Mittler R,Vanderauwera S,Suzuki N,et al. ROS signaling:the new wave?[J]. Trends in Plant Science,2011,16(6):300-309.
19. Suzuki N,Miller G,Morales J,et al. Respiratory burst oxidases:the engines of ROS signaling[J]. Current Opinion in Plant Biology,2011,14(6):691-699.
20. 郭盈天,张泽,付强,等. 高温胁迫对金露梅叶片结构和生理代谢的影响[J]. 北方园艺,2018,23:83-98.
Guo Y T,Zhang Z,Fu Q,et al. Effects of high temperature stress on leaf structures and physiogical metabolism of Potentilla fruticosa L.[J]. Northern Horticulture,2018,23:93-98.
21. Gill S S,Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiology and Biochemistry,2010,48(12):909-930.
22. 李晶,阎秀峰,祖元刚. 低温胁迫下红松幼苗活性氧的产生及保护酶的变化[J]. 植物学报,2000,42(2):148-152.
Li J,Yan X F,Zu Y G. Generation of activated oxygen and change of cell defense enzyme activity in leaves of Korean Pine seedling under low temperature[J]. Acta Botanica Sinica,2000,42(2):148-152.
23. 时丽冉,白丽荣,吕亚慈,等. 小麦杂交品种衡9966苗期耐盐性分析[J]. 作物杂志,2018,(6):149-153.
Shi L R,Bai L R,Lü Y C,et al. Analysis of salt tolerance at the seedling stage of wheat hybrid variety Heng 9966[J]. Crops,2018,(6):149-153.
24. Zhao J,Davis L C,Verpoorte R. Elicitor signal transduction leading to production of plant secondary metabolites[J]. Biotechnology Advances,2005,23(4):283-333.
25. O'donnell P J,Calvert C,Atzorn R,et al. Ethylene as a signal mediating the wound response of tomato plants[J]. Science,1996,274(5294):1914-1917.
26. Avanci N C,Luche D D,Goldman G H,et al. Jasmonates are phytohormones with multiple functions,including plant defense and reproduction[J]. Genetics and Molecular Research,2010,9(1):484-505.
27. 刘艳艳,萧凤回. JAs、SAs介导的植物防御反应及在药用植物中的应用[J]. 中国农学通报,2010,26(14):98-100.
Liu Y Y,Xiao F H. Jasmonate and Salicylate-mediated plant defense responses and their application in medicinal plants[J]. Chinese Agricultural Science Bulletin,2010,26(14):98-100.
28. 陈少裕. 膜脂过氧化对植物细胞的伤害[J]. 植物生理学通讯,1991,27(2):84-90.
Chen S Y. Injury of membrane lipid peroxidation to plant cell[J]. Plant Physiology Communication,1991,27(2):84-90.
29. 刘晓英,焦学磊,徐志刚,等. 不同红蓝LED光照强度对樱桃番茄幼苗生长和抗氧化酶活性的影响[J]. 南京农业大学学报,2015,38(5):772-779.
Liu X Y,Jiao X L,Xu Z G,et al. Effects of different red and blue LED light intensity on growth and antioxidant enzyme activity of cherry tomato seedlings[J]. Journal of Nanjing Agricultural University,2015,38(5):772-779.
30. Lindquist S,Craig E A. The heat-shock proteins[J]. Annual Review of Genetics,1988,22(1):631-677.
31. Rajput V D,Chen Y,Ayup M. Effects of high salinity on physiological and anatomical indices in the early stages of Populus euphratica growth[J]. Russian Journal of Plant Physiology,2015,62(2):229-236.
32. 徐维娜. 真菌侵染诱导沉香形成关键技术效果评价及结香机制初步研究[D]. 广州:广东药学院,2011,9.
Xu W N. Evaluation on key technology of fungi infection-induced aloes-forming effect and preliminary research on the mechanism of the eaglewood formation[D]. Guangzhou:Guangdong Pharmaceutical University,2011,9.
33. 崔之益,徐大平,杨曾奖,等. 心材形成机理与人工促进研究进展[J]. 世界林业研究,2016,29(6):33-37.
Cui Z Y,Xu D P,Yang Z J,et al. A review of mechanism and artificial promotion of heartwood formation[J]. World Forestry Research,2016,29(6):33-37.
34. Ziegler H. Biologische aspekte der kernholzbildung[J]. Holz als Roh-und Werkstoff,1968,26(2):61-68.
35. 诸葛强,黄敏仁,潘惠新,等. 杨树湿心材的化学特性及形成机理研究[J]. 林业科学,1997,33(3):259-266.
Zhu G Q,Huang M R,Pan H X,et al. Study on chemical characteristics and formation mechanism of poplar wetheartwood[J]. Scientia Silvae Sinicae,1997,33(3):259-266.

[1]	Yuping QIU, Yichuan WANG, Hongwei GUO. Research Progress on the Regulatory Mechanism of Plant Root Hair Development [J]. Bulletin of Botanical Research, 2023, 43(3): 321-332.
[2]	Kun CHEN, Gonggui FANG, Huaizhi MU, Jing JIANG. Analysis of the Promoter Sequence and Response Characteristics of the BpPIN3 gene in Betula platyphylla [J]. Bulletin of Botanical Research, 2022, 42(4): 592-601.
[3]	Bing GAO, Pengfei GAO, Weifang FAN, Zhenghong FENG, Jianhui WU. Effects of Cadmium Stress on the Root Structure and Physiology of Symbiont of Potentilla sericea and Arbuscular Mycorrhizal fungi [J]. Bulletin of Botanical Research, 2022, 42(4): 647-656.
[4]	Liben PAN, Xue YAN, Jia LIU, Kexin WU, Yang LIU, Shaochong LIU. Physiological Characteristics of Early Spring Flowering Plants under Northeast Forest [J]. Bulletin of Botanical Research, 2022, 42(4): 657-666.
[5]	Guobin Liu, Ting Liao, Ye Wang, Liqin Guo, Jinzhe Zhao, Yanwu Yao, Jun Cao. Regulation Mechanism of Endogenous Hormones in Adventitious Roots Formation of Platycladus orientalis ‘Beverleyensis’ [J]. Bulletin of Botanical Research, 2022, 42(2): 278-288.
[6]	Yang-Jing ZHAO, Nan TANG, Dao-Cheng TANG, Jing ZHANG. An Optimized HPLC Procedure for Analyzing Three Endogenous Hormones in Tulip Bulbs [J]. Bulletin of Botanical Research, 2021, 41(1): 130-137.
[7]	WANG Ning, DONG Ying-Ying, YUAN Mei-Li, WANG Ya-Nan, ZENG Sheng-Yun. Effect of Warm Water Soaking on Protective Enzyme Activity and Endogenous Hormones Content of Eucommia ulmoides Seed During Different Stages of Germination [J]. Bulletin of Botanical Research, 2020, 40(4): 523-529.
[8]	YANG Liu, WANG Le, XIE Zhong-Kui, GUO Zhi-Hong, ZHANG Yu-Bao. Influence of Endogenous IAA and GA₃ on in vitro Lily Seedling Growth [J]. Bulletin of Botanical Research, 2016, 36(5): 760-767.
[9]	DENG Shao-Li;JIANG Guo-Bin;REN Xian;JIN Hua;MA Jin-Long;ZOU Ji-Xiang. Responses of Endogenous Hormones to Drought Stress in the Apoplast of Populus* [J]. Bulletin of Botanical Research, 2013, 33(6): 690-696.
[10]	GUO Qi;ZHANG Jun;ZHAO Xiao-Feng;YANG Tao;WANG Pei-Ya;YANG Hui;. In vitro* Culture of Endangered Medicinal Plant Sinopodophyllum hexandrum Royle [J]. Bulletin of Botanical Research, 2012, 32(4): 484-487.
[11]	HE Yue-Jun;ZHONG Zhang-Cheng;;LIU Jin-Chun;LIU Ji-Ming. Photosynthetic Characteristics of Broussonetia papyrifera* Seedlings Inoculated AM Fungus in Limestone Soil Substratum [J]. Bulletin of Botanical Research, 2008, 28(4): 452-457.
[12]	SUN Hong-Mei;LI Tian-Lai;LI Yun-Fei. Changes of endogenous hormones in Lilium davidii* var. unicolor bulbs during bulb development and storage at low temperature for dormancy release [J]. Bulletin of Botanical Research, 2006, 26(5): 570-576.
[13]	LI Yan;JIANG Zai-Min;TANG Rui. Phytohormones combination on leaf callus induction of Eucommia ulmoides [J]. Bulletin of Botanical Research, 2006, 26(2): 182-186.
[14]	AO Hong, WANG Kun, FENG Yu-long. ENDOGENOUS HORMONES LEVELS IN CUTTINGS OF LARIX OLGENSIS AND THEIR RELATIONS TO ROOTING [J]. Bulletin of Botanical Research, 2002, 22(2): 190-195.
[15]	Xue Yu, Li Zhun-yan, Zhang Ming, Xiao Xiang-Yang. A NEW RECORD SPECIES OF HYALOPSORA IN CHINA [J]. Bulletin of Botanical Research, 1997, 17(1): 54-55.