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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (3): 458-465.doi: 10.7525/j.issn.1673-5102.2019.03.016

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External Antioxidant Activity of Ethanol Extract from Pinus sylvestris var. mongolica

ZHANG Li-Fan1, XUE Yu1, WANG Yan2, YANG Guang-Xin3, YANG Guang1, DI Xue-Ying1   

  1. 1. School of forestry, Northeast Forestry University, Harbin 150040;
    2. Forest Farm, Xunke County Road, Heihe 164499;
    3. National Forestry Bureau Daxing'an Mountains Forestry Survey Planning and Design Institute, Gagdage 165000
  • Received:2019-03-13 Online:2019-05-05 Published:2019-05-11
  • Supported by:
    The open fund project of Beijing key laboratory(BJFUKF201818)

Abstract: The ethanol extractantioxidant activity of cone, bark, leaf, xylem four parts of Pinus sylvestris var. mongolica was compared to provide theoretical basis for further exploitation and utilization of pine resources. P.sylvestris var. mongolica bark, leaves, cones, xylem four parts as raw material, the content of polyphenols and flavonoids in the ethanol extract of camphor was determined. Determination of the P.sylvestris var. mongolica ethanol extracts of polyphenols, flavonoids, and ascorbic acid(VC) is applied to make positive control evaluation of the extracts from different parts of total antioxidant capacity, total reducing power, diphenyl bitter acyl(DPPH·) cleaning ability, hydroxyl free radical(·OH) clear, super oxygen anion(O2·) remove the five indicators, evaluation of pine ethanol extractpolyphenols ofmongolica four parts in vitro antioxidant activity, draw standard curve. The results showed that:(1)mongolica polyphenols extracted from P.sylvestris var. mongolica bark showed strong antioxidant capacity, in relatively high concentrations and antioxidants(Vc) is smaller; The camphor pine polyphenols, extracted from camphor cones, also exhibit stronger restorative powers, even more than antioxidants(Vc); (2)P.sylvestris var. mongolica polyphenols extracted from which bark in diphenyl bitter acyl(DPPH·) cleaning ability, hydroxyl free radical(·OH) removal ability are the highest of the three, but no more than antioxidants(Vc) antioxidant capacity; (3)super oxygen anion(O2·) cleaning ability index, the clearance of all negative, shows that the method for determining the ultra oxygen anion(O2·) the clearance interference factors are unable to compare three size on this metric capability. Experimental results show that the polyphenols have strong antioxidant ability, which can be used as a natural antioxidant.

Key words: Pinus sylvestris var. mongolica, polyphenol, ethanol extract, antioxidation activity

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