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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (1): 69-77.doi: 10.7525/j.issn.1673-5102.2017.01.010

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Sequence and Expression Analysis of MADS-box Genes in Celastrus orbiculatus Thunb. Based on RNA-seq Data

ZU Kui-Ling, DONG Shu-Bin, LI Jian-Xia, ZHAO Yun-Yu, ZHAO Liang-Cheng   

  1. School of Nature Reservation, Beijing Forestry University, Beijing 100083
  • Received:2016-07-06 Online:2017-01-15 Published:2017-03-06
  • Supported by:
    This work was supported by funding from the National Natural Science Foundation of China(31370213);Beijing Natural Science Foundation(5133036)

Abstract: For the regulatory information of MADS-box genes on the flower and fruit development in Celastrus orbiculatus Thunb, 15 MADS-box genes(ColMADS) with full ORF(Open Reading Frame)were identified from transcriptome generated from female flower and fruit. The features of the proteins were analyzed by the bioinformatics methods. The comp37814_g1and comp41380_g2 belonged to M-type family, while other 13 genes were the members of MIKC. All were unstable hydrophilic proteins except for comp37814_g1. Alpha helix, beta turn, extended strand and random coil were found from the secondary structure of ColMADS protein, and alpha helix was major part. Five motifs were identified. The Motif 1 and Motif 2, both consisting of 50 amino acids, were the conserved structure of MADS-box family and only Motif 1 contained MADS domain. By phylogenetic analysis, 15 ColMADS genes were divided into 10 clades, belonging to different subfamilies and groups. In addition, the expression patterns of the genes among different development stages were analyzed using real-time RT-PCR. Two genes were highly expressed at flower blooming stage and nine genes were obviously expressed at flower senescence stage. There are four genes upregulated during the young fruit development.

Key words: Celastrus orbiculatus Thunb., MADS-box genes, female flower, sequence and expression analysis

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