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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (1): 134-140.doi: 10.7525/j.issn.1673-5102.2016.01.019

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Clone and Characterization of A Glutathione-s-transferase Gene in Muscari armeniacum

YANG Hui-Ping;LIU Ya-Li*;LOU Qian;LIU Ni-Ni   

  1. State Key Laboratory of Crop Stress Biology in Arid Areas,Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,Ministry of Agriculture,College of Forestry,Northwest A&F University,Yangling 712100
  • Online:2016-01-15 Published:2016-05-18

Abstract: Based on the latex the transcriptome database, the full length cDNA of glutathione-S-transferase(GST) from Muscari armeniacum was cloned by reverse-PCR and PCR, designated as MaGST. The full length cDNA of MaGST was 711 bp, and the ORF(Open Reading Frame) length was 666 bp, encoding a protein polypeptide of 221 amino acids with a predicted molecular weight of 54.1 kD and pI of 5.13. By phylogenetic tree analysis, the putative MaGST protein displayed identities to the GSTs of Allium cepa and Triticum aestivum of 76.72% and 62.50%, respectively, and contained the Tau GST-specific N-terminal domain(G site) and the C-terminal domain(H site), belonging to the family of GST Tau. By realtime PCR, the expression pattern of MaGST in different organs were similar, belonging to constitutive expression. The expression of MaGST was regulated by salicylic acid, but not by the NaCl.

Key words: Muscari armeniacum, glutathione-s-transferase, clone, florescent real-time quantitative PCR, environment stress