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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (6): 825-831.doi: 10.7525/j.issn.1673-5102.2015.06.006

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Establishment and Optimization of Wild Beltilla striata Regeneration System

LI Fang1;XIAO Xiao-Jun1*;LIN Zhong-Quan2;DU Ming-Lin1;HU Zhang-Wei1;HUANG Zuo-Xi1   

  1. 1.College of Life Sciences,Neijiang Normal University,Neijiang 641100;
    2.Sichuan Shenda Bio-Technique Co.Ltd.,Nanchong 637000
  • Online:2015-11-20 Published:2016-01-18
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Abstract: We used the ripe capsules from wild Bletilla striata as explants to establish regeneration system. The optimal medium for seed germination was 1/2MS+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1+AC 0.5 g·L-1. The most suitable medium for PLB multiplication was MS+KT 1.0 mg·L-1+NAA 0.1 mg·L-1+AC 0.5 g·L-1, while MS+6-BA 0.5 mg·L-1+KT 1.0 mg·L-1+NAA 0.1 mg·L-1+AC 0.5 g·L-1 was appropriate for PLB differentiation. The optimal medium for adventitious buds multiplication was MS+6-BA 0.5 mg·L-1+KT 1.0 mg·L-1+NAA 0.1 mg·L-1+AC 0.5 g·L-1+potato juice 150 g·L-1, and the proliferation rate was up to 343% after 30 days. The most suitable rooting medium was 1/2MS+IBA 0.5 mg·L-1+AC 0.5 g·L-1+potato juice 100 g·L-1 with the rooting rate of 100%. The best way of tube seedlings transplanting was under the volume ratio of sphagna: batk: Coconut chaff: wood flour of 2∶2∶1∶1 with 99% of survival rate.

Key words: Bletilla striata, regeneration system, optimization

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