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Bulletin of Botanical Research ›› 2010, Vol. 30 ›› Issue (3): 373-378.doi: 10.7525/j.issn.1673-5102.2010.03.022

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Separation and Purification Process of Total Flavonoids from Elaeagnus mollis Leaves

SUI Yu-Xiang;ZHANG Jia-Ming;MU Nan;DU Zhen;YAN Gui-Qin*   

  1. School of Life Science,Institue of Biodiversity,Shanxi Normal University,Linfen 041004
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Contact: YAN Gui-Qin
  • Supported by:

Abstract: The content of total flavonoids in Elaeagnus mollis Diels leaves was determined by ultraviolet spectrophotometry,the adsorption capacity and desorption rate of four kinds of macroporous resins(D4020、AB-8、S-8、X-5) were calculated to determine the suitable resin and its optimum process conditions. The results indicated: S-8 resin had the highest adsorption capacity(9.83 mg·g-1), followed by AB-8(9.38 mg·g-1), but the desorption rate of AB-8 resin(88.79%) was better than that of S-8 resin(71.89%).AB-8 resin was the best absorbent for the total flavonoids in E.mollis leaves. The optimum process conditions were: the pH value of extracts was 3.0~4.0, the ratio of absorbing solution to resin was 6∶1, the adsorption flow rate was 2 mL·min-1, the eluting solution was 60% ethanol, the eluting flow rate was 2 mL·min-1, the volume of the eluting solution was 3BV.

Key words: Elaeagnus mollis, leaves, total flavonoids, macroreticular absorption resin, separation and purification

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