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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (4): 509-512.doi: 10.7525/j.issn.1673-5102.2009.04.022

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Preliminary Study on Anther Culture of Erigeron breviscapus

ZHANG Zhi-Hui;ZHAO Zhen-Ling;YANG Wei-Ze;ZHANG Jin-Yu;YANG Mei-Quan;JIN Hang*   

  1. (Medicinal Plant Institute,Yunnan Academy of Agricultural Sciences,Kunming650231)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-20 Published:2009-07-20
  • Contact: JIN Hang
  • Supported by:

Abstract: The preliminary study was carried out on the anther culture of Erigeron breviscapus. The results showed that during callus induction, 60 g·L-1 sucrose was suitable;MS medium was better than B5; the highest induction efficiency 36.03% was achieved on the medium of MS+NAA 1.0 mg·L-1+BA 0.5 mg·L-1+sucrose 60 g·L-1. calli were transferred on medium MS+6-BA 1.0 mg·L-1 to proliferation. After differentiating and taking roots, plantlets were obtained. The chromosome numbers from root-tip cells of regeneration plant was nine. It showed that the regeneration plant was haploid.

Key words: Erigeron breviscapus(Vant)Hand.-Mazz, anther culture, haploid

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